Method for detecting trace estrogen substances in water environment

A detection method and estrogen technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as deviation of sample concentration test results and poor applicability of detection methods, so as to achieve true and reliable detection results, reduce detection costs, and reduce reagents. Simple and cost-effective

Inactive Publication Date: 2021-11-26
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

However, the complex environmental matrix often produces large deviations in the test results of the sample concentration, which in turn leads to poor applicability of the detection method

Method used

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  • Method for detecting trace estrogen substances in water environment
  • Method for detecting trace estrogen substances in water environment
  • Method for detecting trace estrogen substances in water environment

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Embodiment

[0040] This embodiment adopts the internal standard method to test the estrogen in the influent and effluent water of deionized water, tap water, surface water, and livestock and poultry breeding wastewater treatment process simultaneously by the solid phase extraction pretreatment combined with liquid mass spectrometry technology of the present invention Calibration curve linearity, LOD and LOQ for species species. Add a mixed standard solution of estrogens (the mixed standard solution is a mixed standard stock solution of 6 estrogens, the concentration of which is 1000 mg / L) to the environmental samples to make the enriched calibration curve based on 6 concentrations The levels were 4 μg / L, 10 μg / L, 20 μg / L, 50 μg / L, 100 μg / L, and 200 μg / L (the enrichment factor was 250 times that of deionized water and tap water, and that of surface water was 500 times, the influent and effluent of the livestock and poultry breeding wastewater treatment process are 50 times).

[0041]The...

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Abstract

The invention provides a method for detecting trace estrogen substances in a water environment. The method comprises the following steps: filtering a water sample through a glass fiber membrane to remove insoluble suspended particles, adding EDTA-2Na for dissolving, adding an estrogen internal standard mixed stock solution, and adjusting the pH value; enriching target estrogen by using a treated water sample through solid-phase extraction, leaching a solid-phase extraction column by using a leacheate under negative pressure, eluting a target object by using a certain volume of an elution solvent under normal pressure, collecting the eluate, carrying out nitrogen blowing, and fixing the volume to obtain a to-be-detected estrogen solution; and quantitatively detecting the content of the enriched estrogen substances in the water environment on a high performance liquid chromatography tandem mass spectrometer by adopting an internal standard method. According to the detection method disclosed by the invention, solid-phase extraction is combined with liquid chromatography-tandem mass spectrometry, and solid-phase extraction conditions are optimized, so that the detection method can be used for rapidly, accurately and efficiently detecting the estrogen substances in various water environments such as tap water, surface water and livestock and poultry breeding wastewater, and meanwhile, the estrogen substances can be detected in various water bodies.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of trace organic matter in water environment, and in particular relates to a detection method of trace estrogen substances in water environment. Background technique [0002] Endocrine disruptors (EDCs) are a class of exogenous substances that interfere with the synthesis, excretion and activity of hormones in organisms. EDCs as natural and synthetic persistent chemicals include pesticides, heavy metals and hormones. Among the hormones, estrogen has attracted extensive attention due to its wide variety, high reactivity, high frequency of existence, and certain concentration in the aquatic environment. According to its source, estrogen can be divided into natural estrogens such as estrone, 17β-estradiol, and estriol, and synthetic estrogens such as ethinyl estradiol and dienestrol. In addition, some chemical additives used in plastic factories, such as bisphenol A, can act as estro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/08G01N30/72G01N30/86
CPCG01N30/88G01N30/06G01N30/08G01N30/72G01N30/8665G01N2030/8809G01N2030/027G01N2030/062G01N2030/085G01N2030/045
Inventor 杨林燕曹国民蔡正清赵慧慧李晔瑾谢颖绮朱琳
Owner EAST CHINA UNIV OF SCI & TECH
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