Method for detecting M protein in serum based on MADLI-TOF MS technology
A serum and protein technology, applied in the field of biomedicine, can solve the problems of increasing the detection cost, increasing the complexity of the operation, reducing the reduction, etc., and achieves the effects of simple operation, improved prognosis and high detection sensitivity.
Pending Publication Date: 2021-11-30
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology
The detection speed of this method is much higher than that of PEL and IFE, and the mass spectrometry detection time of each sample is less than 1 minute; the detection cost is between PEL and IFE; it is not interfered by fibrinogen and β-region monoclonal immunoglobulin, these Advantages It has the basic requirements of high-throughput screening tests, but the immun
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[0052] Instruments, reagents and materials
[0053] 1 instrument
[0054] 1.1 QuanTOF wide-spectrum quantitative time-of-flight mass spectrometer (China, Rongzhi Bio), which belongs to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (Matrix-assisted laser desorption / ionization time-of-flightmass spectrometry, MALDI-TOF MS)
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The invention provides a method for detecting M protein in serum based on an MADLI-TOF MS technology. The rapid screening method for directly detecting the serum M protein based on the MALDI-TOF MS technology has the advantages of being rapid, high in throughput, easy and convenient to operate and clear and easy in spectrogram interpretation, and the detection sensitivity of the rapid screening method is higher than that of an existing M protein screening technology; the method is expected to play an important role in screening, diagnosis, curative effect evaluation, prognosis judgment and tiny residue detection of multiple myeloma.
Description
technical field [0001] The invention relates to a method for detecting M protein in serum based on MADLI-TOF MS technology, which belongs to the field of biomedicine. Background technique [0002] Multiple myeloma (multiple myeloma, MM) is still incurable. Early identification of its main biological indicators and timely effective intervention are important means for accurate diagnosis, curative effect evaluation and prolongation of survival. The appearance of M protein in serum is a prominent feature of this disease, and the concentration of M protein is related to the severity of clinical symptoms. Early identification of M protein and timely and effective intervention to track the disease process and treatment response have important guiding significance for clinical diagnosis and treatment. Unlike most serological tumor markers, M proteins are very diverse. Each M protein has a unique variable region sequence, ranging in molecular weight from pentameric IgM (~900,000 Da...
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Login to view more IPC IPC(8): G01N27/64G01N1/28G01N1/38G01N33/574G01N33/68
CPCG01N27/64G01N1/28G01N1/38G01N33/57407G01N33/57488G01N33/68
Inventor 张瑞崔瑞芳王清涛翟玉华张顺利岳育红王默
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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