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Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling

A UV-A, pakchoi technology, applied in the field of light regulation, can solve the problems of lack of metabolism level, gene level, etc.

Pending Publication Date: 2021-12-03
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Secondly, the effects of different light qualities on plant growth and nutritional quality are mostly studied at the physiological level, but there is a lack of research at the metabolic and genetic levels.

Method used

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  • Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling
  • Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Using pak choy as an experimental raw material, the method for carrying out photoregulation and anthocyanin content measurement of pak choy comprises the following steps:

[0035] S1. Select the pakchoi seeds required for the experiment, and select excellent pakchoi seeds with high survival rate, then soak the seeds in sterile water, then embellish them on the germination paper, transfer them to the dark for accelerated germination, Then select the dew-white seeds and sow them on the moist sponge block to carry out the work of raising seedlings;

[0036] S2. For the pakchoi seedlings cultivated in step S1, select robust and consistent seedlings to be fixed in the hydroponic box, and artificially intervene in the temperature and humidity of the seedling room and the climate chamber. The light treatments tested in the experiment are different light treatments. Strong UV-A and blue light;

[0037] S3. For the pakchoi seedlings cultivated in step S1, deploy the nutrient ele...

Embodiment 2

[0049] The method of photoregulation and anthocyanin content measurement of pakchoi provided in this example is roughly the same as that of Example 1, the main difference is that in step S1, the soaking time in sterile water is 30 minutes, and the dark germination time is 24 hours;

[0050] In step S2, the temperature in the nursery room and the artificial climate chamber is 25° C., and the humidity is 85° C.

[0051] The photoperiod in step S2 is ten hours per day.

[0052] The nutrient solution prepared in step S3 is made of potassium nitrate, calcium nitrate, ammonium dihydrogen phosphate and magnesium sulfate, wherein the concentration of soluble ions is 1.3ds / m, and the pH is 6.0.

[0053] In step S5, the centrifugal temperature of the centrifuge is 4° C., the rotational speed of the centrifuge is 12000 rpm, and the separation time of the centrifuge is 30 minutes.

Embodiment 3

[0055] The method of photoregulation and anthocyanin content measurement of pakchoi provided in this example is roughly the same as that of Example 1, the main difference being that in step S1, the soaking time in sterile water is 45 minutes, and the dark germination time is 24 hours;

[0056] In step S2, the temperature in the nursery room and the artificial climate chamber is 24°C, and the humidity is 80°C.

[0057] The photoperiod in step S2 is thirteen hours per day.

[0058] The nutrient solution prepared in step S3 is made of potassium nitrate, calcium nitrate, ammonium dihydrogen phosphate and magnesium sulfate, wherein the concentration of soluble ions is 1.2ds / m, and the pH is 5.9.

[0059] In step S5, the centrifugal temperature of the centrifuge is 4° C., the rotational speed of the centrifuge is 12000 rpm, and the separation time of the centrifuge is 25 minutes.

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Abstract

The invention relates to the technical field of light regulation and control, in particular to a molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling. According to the method, during an experiment, excellent pakchoi seeds are firstly obtained, cultivation experiment work is carried out, finally obtained experiment data are more accurate, the credibility of the experiment is improved, then the seeds are placed in sterile water to be soaked, the seeds can rapidly absorb water, the germination time of the seeds is shortened, meanwhile, sterile water is used for soaking the seeds, the influence of bacteria on the seeds can be reduced when the seeds absorb water, the survival rate of the seeds is increased, meanwhile, the temperature and humidity of a seedling chamber and a phytotron are manually adjusted, the temperature and humidity are manually adjusted to be suitable for the growth of the pakchoi, the growth speed of the pakchoi is increased, during growth, UV-A coupling and blue light with different light intensities are used for light regulation and control work, and the anthocyanin component rich in the pakchoi is increased along with the change of time.

Description

technical field [0001] The invention relates to the technical field of light regulation, in particular to a molecular mechanism research method for anthocyanin accumulation in Chinese cabbage based on UV-A coupling. Background technique [0002] Plants capture light energy through the photosynthetic pigments of the leaves and convert it into chemical energy through the electron transport chain, which is used for the synthesis of organic matter in the body. Plants cannot complete a series of life activities without energy action and signal transduction. Therefore, plant vegetative growth and reproductive growth The whole process depends on the lighting environment. With the deepening of the physiology and mechanism of plant light quality, researchers are gradually exploring the light formula that meets the requirements of plant growth and quality control, and many important conclusions have been drawn from this. [0003] At present, natural light acts on plants in the form o...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G22/15A01G7/06G01N21/31
CPCA01G31/00A01G22/15A01G7/06G01N21/31
Inventor 郑胤建许亚良李清明卞中华王森
Owner INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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