Nucleic acid extractor based on magnetic bead method

Abstract

The invention relates to a nucleic acid extractor based on a magnetic bead method, which comprises a magnetic rod assembly, a magnetic rod sleeve assembly located at the lower end of the magnetic rod assembly and used for sleeving the magnetic rod assembly, a first driving mechanism used for bearing the magnetic rod assembly to move along the up-down direction, a second driving mechanism used for bearing the magnetic rod sleeve assembly to move along the up-down direction, a test tube box arranged on the lower portion of the magnetic rod sleeve assembly, and a third driving mechanism used for bearing the test tube box to move in the horizontal direction. The magnetic rod assembly can be repeatedly inserted into or extend out of the magnetic rod sleeve assembly under the action of the first driving mechanism, after the magnetic rod sleeve assembly enters a test tube, under the vibration action of repeated insertion or extension of the magnetic rod assembly, the magnetic rod sleeve assembly has magnetism or does not have magnetism, intense vibration of magnetic beads is achieved, nucleic acid extraction can be efficiently completed conveniently, and the success rate and efficiency are greatly improved.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction and purification, in particular to a nucleic acid extractor based on a magnetic bead method. Background technique [0002] Nucleic acid extraction is the first step in subsequent experiments or research such as molecular diagnostic experiments. After the nucleic acid is extracted and purified, subsequent research can be carried out, and this is also a very important link, which shows the importance of nucleic acid extraction and purification. At present, instruments for nucleic acid extraction have been developed, such as Kingfisher Flex produced by Thermo Fisher, etc. These instruments are widely used in various fields such as genomics, disease control, medical treatment, food safety, and forensic identification. [0003] The magnetic bead method for nucleic acid extraction, based on the same principle as the silica gel membrane spin column, uses nanotechnology to improve and modify the sur...

AI Technical Summary

Problems solved by technology

Utilizing the superparamagnetism of nano-magnetic microspheres coated with silica, under the action of Chaotropic salt (guanidine hydrochloride, guanidine isothiocyanate, etc.) DNA and RNA can be isolated from DNA, which can be used in various fields such as clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, molecular biology research, etc. However, the success rate and efficiency of magnetic bead extraction of nucleic acid still remain relatively low and cannot be quantified

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