Supercharge Your Innovation With Domain-Expert AI Agents!

Composition comprising lactobacillus reuteri atg-f4 for prevention or treatment of muscular disorder

A technology of ATG-F4 and Lactobacillus iastii, applied in muscular system diseases, neuromuscular system diseases, drug combinations, etc., can solve the problems of high protein diet, side effects, excessive protein intake, etc., and improve muscle recovery and the effect on exercise capacity

Pending Publication Date: 2021-12-03
ATOGEN CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this can also lead to excessive protein intake, it has a high potential for side effects
In addition, high protein diets may not be achievable due to the presence of kidney disease and the decline in renal function with age, so alternatives to high protein diets are needed as a means of preventing sarcopenia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition comprising lactobacillus reuteri atg-f4 for prevention or treatment of muscular disorder
  • Composition comprising lactobacillus reuteri atg-f4 for prevention or treatment of muscular disorder
  • Composition comprising lactobacillus reuteri atg-f4 for prevention or treatment of muscular disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0054] Isolation and identification of embodiment 1.ATG-F1, ATG-F3 and ATG-F4 lactic acid bacteria

[0055] Samples were prepared from three donated neonatal feces on January 8, 2016, May 31, 2018, and June 4, 2018, and were diluted with 0.9% saline by 10-fold serial dilution, and The diluted samples were plated on de Man, Rogosa, Sharpe (MRS) medium and incubated at 37°C for about 48 hours.

[0056] Colonies of lactic acid bacteria formed on the incubated MRS medium were observed under a microscope to select for bacteria showing bacillus morphological and catalase-negative colonies. Whole genome sequencing was performed and the strains were named ATG-F1, ATG-F3 and ATG-F4 (also known as F1, F3 and F4, respectively).

[0057] 16S rRNA sequencing of ATG-F1, ATG-F3 and ATG-F4 strains was entrusted to Solgent Co., Ltd. (Daejeon). will primer

[0058] 27F(5'-AGA GTT TGA TCC TGG CTC AG-3') 518F(5'-CCA GCA GCC GCG GTA ATAC-3'), 907R(5'-CCG TCA ATT CMT TTR AGT TT-3'), 1492R (5′...

Embodiment approach 2

[0073] Embodiment 2. Cultivation of Lactobacillus lactic acid bacteria

[0074] In this embodiment, Lactobacillus casei ATG-F1, Lactobacillus reuteri ATG-F3 and Lactobacillus reuteri ATG-F4 (accession number KCTC13717BP) were used. Each lactic acid bacteria was mixed in MRS broth (Difco TM Lactobacilli MRS Broth) were cultured at 37°C for 24 hours, and the cultured lactic acid bacteria were centrifuged at 12,000 g for 10 minutes to obtain only the cells, which were then suspended in 0.85% (v / v) saline for each experiment.

experiment example 1

[0075] Experimental example 1. Determination of activation of muscle recovery in sarcopenia-induced model

[0076] Before performing all animal experiments according to this experimental example, animal experiments were supervised by the Institutional Animal Care and Use Committee of AtoGEN Co., Ltd. ( IACUC) approved according to the appropriate procedure (approval number: ATG-IACUC-REV-180810) and provides guidance on ethical animal experiments. Animals used in this experiment were 4-week-old C57BL / 6J purchased from Central Lab Animal Inc. (Seoul, Korea) for experiments.

[0077] In this experimental example, an animal experiment was conducted to study sarcopenia induction model (skeletal muscle atrophy model induced by fixation using a skin stapler in mice) depending on ingestion of Lactobacillus casei ATG - Muscle rehabilitation and improvement of exercise capacity in F1, L. reuteri ATG-F3 or L. reuteri ATG-F4 lactic acid bacteria.

[0078] A total of 25 4-week-old C57B...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a composition comprising Lactobacillus reuteri ATG-F4 strain for prevention or treatment of sarcopenia. More specifically, the strain exhibits an effect of improving muscle rehabilitation and motor ability by increasing the muscle mass in skeletal muscles and as such, can be used in a composition for prevention or treatment of sarcopenia or muscular atrophy.

Description

technical field [0001] The present invention relates to a composition for preventing or treating muscle disorders, said composition comprising Lactobacillus reuteri ATG-F4 strain. More specifically, the present invention relates to a composition for preventing or treating sarcopenia, the composition comprising Lactobacillus reuteri ATG-F4. Background technique [0002] Recently, South Korea has rapidly entered an aging society due to the increase in average life expectancy. As of 2018, the number of people aged 65 and over was 7,381,000, accounting for 14.3% of the entire population (2018 Elderly Statistics, Statistics Korea As aging progresses, many physical changes occur, and the most representative of these biological changes is sarcopenia. [0003] In general, the best way to prevent sarcopenia in everyday life is to prevent muscle loss by continuing to use the muscles, but it is also possible when the muscles become unusable regardless of one's will, for example in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P21/00A23L33/135
CPCA61P21/00A23L33/135A61K35/747A61K2035/115A23V2002/00A23V2200/316C12R2001/225
Inventor 郑渡润白宝篮姜智姬李镕贤朴建锡任成薰宋元镐
Owner ATOGEN CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com