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Method for Vitrification of Bovine IVF Embryos

A vitrification and embryo technology, applied in the field of bovine in vitro fertilization embryo vitrification and bovine in vitro fertilization, can solve the problem of low embryo survival rate

Active Publication Date: 2022-02-08
天津力牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason CN109699636A has proposed a kind of vitrification embryo freezing method and the packing scheme that is applicable to this method, although this document presents excellent effect in some respects, yet the inventor is carrying out vitrification freezing of bovine in vitro fertilization embryo by referring to this method New difficulties are still encountered, such as the defect of low survival rate of embryos obtained after in vitro development of bovine fertilized eggs after undergoing freezing and thawing

Method used

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  • Method for Vitrification of Bovine IVF Embryos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, bovine in vitro fertilization embryo vitrification freezing

[0037] The present embodiment method comprises the steps:

[0038] (1) Provide vitrification liquid, freezing pretreatment liquid, thawing liquid, and cryogenic tubes;

[0039] (2) Apply force through the suction end of the freezing tube, and suck in the tube from the inlet end of the freezing tube in turn: 7 cm of thawing liquid, 0.75 cm of air, 0.5 cm of vitrified liquid, 0.75 cm of air, and 0.5 cm of vitrified liquid containing embryos. cm, air 0.75cm, thawing liquid 0.75cm;

[0040] (3) Seal the upper part of the thawing liquid at the upper end and the lower part of the thawing liquid at the lower end respectively, so that the seven sections sucked in step (2) are sealed in the freezing tube, and then the freezing tube is frozen in liquid nitrogen.

[0041] In the above steps,

[0042] After the two ends of the freezing tube are sealed, the ratio of the total length of the thawing liquid ...

Embodiment 2

[0048] Embodiment 2, bovine in vitro fertilization embryo vitrification freezing

[0049] The present embodiment method comprises the steps:

[0050] (1) Provide vitrification liquid, freezing pretreatment liquid, thawing liquid, and cryogenic tubes;

[0051] (2) Apply force through the suction end of the freezing tube, and suck in the tube from the inlet end of the freezing tube in turn: thawing liquid 8cm, air 1cm, vitrification liquid 0.4cm, air 0.5cm, vitrification liquid containing embryos 0.6cm , air 0.5cm, thawing liquid 1cm;

[0052] (3) Seal the upper part of the thawing liquid at the upper end and the lower part of the thawing liquid at the lower end respectively, so that the seven sections sucked in step (2) are sealed in the freezing tube, and then the freezing tube is frozen in liquid nitrogen.

[0053] In the above steps,

[0054] After the two ends of the freezing tube are sealed, the ratio of the total length of the thawing liquid to the total length of the ...

Embodiment 3

[0059] Embodiment 3, bovine in vitro fertilization embryo vitrification freezing

[0060] The present embodiment method comprises the steps:

[0061] (1) Provide vitrification liquid, freezing pretreatment liquid, thawing liquid, and cryogenic tubes;

[0062] (2) Apply force through the suction end of the freezing tube, and suck in the tube from the inlet end of the freezing tube in turn: 6 cm of thawing liquid, 0.5 cm of air, 0.6 cm of vitrified freezing liquid, 1 cm of air, and 0.4 cm of vitrified freezing liquid containing embryos , air 1cm, thawing liquid 0.5cm;

[0063] (3) Seal the upper part of the thawing liquid at the upper end and the lower part of the thawing liquid at the lower end respectively, so that the seven sections sucked in step (2) are sealed in the freezing tube, and then the freezing tube is frozen in liquid nitrogen.

[0064] In the above steps,

[0065] After the two ends of the freezing tube are sealed, the ratio of the total length of the thawing ...

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Abstract

The invention relates to a vitrification freezing method for bovine in vitro fertilized embryos. Specifically, the method of the present invention includes the following steps: (1) providing vitrification liquid, freezing pretreatment liquid, thawing liquid, and freezing tube; Inhale in the tube: thawing liquid, air, vitrification liquid, air, vitrification liquid containing embryos, air, thawing liquid; (3) seal the upper part of the thawing liquid at the upper end and the lower part of the thawing liquid at the lower end respectively, so that the steps ( 2) The inhaled seven sections are sealed in the cryotube, and then the cryotube is frozen in liquid nitrogen. The method of the present invention exhibits excellent effects as described in the specification.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and relates to a technology for agricultural-animal husbandry and veterinary reproduction, in particular to a method for bovine in vitro fertilization. In addition, the present invention also relates to the use of related working test solutions used in bovine in vitro fertilization. The application in fertilization, in particular, relates to a method of vitrification of bovine in vitro fertilized embryos with excellent results. The bovine IVF embryo vitrification freezing method of the present invention has excellent technical effect. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which mammalian sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. Because it is inseparable from embryo transfer technology (ET), it is also referred to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 王娜赵明礼郝少强陈建美郭春明
Owner 天津力牧生物科技有限公司