Reagent for detecting antibacterial drugs and preparation method thereof

A technology for antibacterial drugs and reagents, which is applied in the field of liquid chromatography tandem mass spectrometry detection to achieve the effects of high responsivity, improved detection efficiency, and improved stability

Active Publication Date: 2021-12-21
江苏豪思睦可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, only voriconazole

Method used

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  • Reagent for detecting antibacterial drugs and preparation method thereof
  • Reagent for detecting antibacterial drugs and preparation method thereof
  • Reagent for detecting antibacterial drugs and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Embodiments of the invention also provides a method for detecting the antimicrobial agent according to the preceding embodiment, comprising: a calibration solution antibacterials liquid chromatography tandem mass spectrometric detection and / or controls in solution were added 3- (N- morpholino) propanesulfonic acid, the solution was lyophilized to obtain a calibrator and / or control materials corresponding lyophilized product.

[0026] Incidentally, the preparation of solutions and drying technology was not added 3- (N- morpholino) propanesulfonic acid solution and controls the calibration solution based on the conventional techniques can acquire knowledge in the art, are not repeated here , as long as there was added 3- (N- morpholino) propanesulfonic acid controls and / or calibrators for detection of antimicrobial agents, is within the scope of protection of the present application.

[0027] In a preferred embodiment, 3- (N- morpholino) propanesulfonic acid solution in...

Example Embodiment

[0049] Example 1

[0050] A liquid chromatographic tandem spectrum detects a kit of a plurality of antibacterial drugs, and the preparation method thereof is as follows.

[0051] Standards: 16 species of antibacterial drugs and stable isotope are purchased from Beijing to make biocons, and the purity is ≥99%.

[0052] At the same time, the target Lienzol (M / Z 338.0 → 296.0), Lanezoline internal standard (M / Z341.0 → 297.0), Melopean (M / Z 384.0 → 141.1), Meilopean internal standard (m / z 390.0 → 147.0), fluconazole (m / z307.0 → 220.0), fluconazole internal standard (m / z 311.0 → 223.2), voriconazole (m / z 350.0 → 280.0), voriconazole internal standard (m / z353 .2 → 127.1), Poshakrazole (M / Z 701.0 → 127.0 / 701.0 → 614.0), Poshakrazole Bid (M / Z 705.0 → 127.0), Irazole (M / Z 705.0 → 392.0 / 705.0 → 432.0), Irazole Bid (M / Z714.0 → 401.0) 卡 芬 芬 Net (M / Z 547.6 → 131.0), Kumole Net Delivery (M / Z 549.5 → 131.0), Mossisha Star (M / Z 402.2 → 384.1), Moxisha buccby (M / ...

Example Embodiment

[0073] Example 2

[0074] A method of detecting a plurality of antibacterial drugs in a liquid chromatography spectrum comprising the following steps.

[0075] 1, pretreatment of plasma / serum sample:

[0076] The pre-treatment of the sample was taken as a sample of the sample, and 100 μl of plasma sample was added, and 100 μl of the internal standard standard was added thereto, and the methanol precipitation agent was added, and after the protein precipitation, the supernatant was subjected to LC-MS / MS. analyze.

[0077] 2, qualitative and quantitative detection:

[0078] The supernatant obtained by treating the sample after protein precipitation method for liquid chromatography-tandem mass spectrometry (LC-MS / MS) analysis, and after testing chromatography, the mass spectrometer is introduced, and the ionized ionization is formed in an ion source. . Under the action of the electric field, focus is focused into the triple four-stage rod quality analyzer. In the first stage fo...

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Abstract

The invention discloses a reagent for detecting antibacterial drugs and a preparation method thereof and relates to the technical field of liquid chromatography-tandem mass spectrometry detection. A quality control product and a calibration product are prepared with 3-(N-morpholine) propanesulfonic acid (MOPS) as a stabilizer, stability of the quality control product and the calibration product can be effectively improved, plasma matrix components are kept to the maximum extent, and the transportation and storage processes of calibration products and quality control products are simplified. When the calibrator and the quality control product prepared by the invention are used for liquid chromatography-tandem mass spectrometry detection of antibacterial drugs, detection of at least 16 drugs can be ensured at the same time, detection efficiency is remarkably improved, and the calibrator and the quality control product are high in responsivity and good in repeatability.

Description

technical field [0001] The invention relates to the technical field of liquid chromatography tandem mass spectrometry detection, in particular to a reagent for detecting antibacterial drugs and a preparation method thereof. Background technique [0002] At present, various antibacterial drugs are widely used in clinical practice. Rational use of antibacterial drugs is crucial to improving the clinical efficacy of anti-infection therapy, shortening the treatment time of patients, and reducing medical expenses. Abuse of antimicrobial drugs or illegal addition of antimicrobial drugs in some Chinese patent medicines and cosmetics will lead to the increase of drug-resistant bacteria and the enhancement of bacterial drug resistance, further exacerbating the threat of infectious diseases to human health. [0003] Currently, there are no systematic clinical testing methods for most antibacterial drugs. Therefore, laboratories still need to develop their own methods to provide hosp...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/04G01N30/34
CPCG01N30/02G01N30/04G01N30/34G01N2030/042G01N2030/045
Inventor 肖冰心栗琳王春静张红王翠竹雒琴丁亮周立
Owner 江苏豪思睦可生物科技有限公司
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