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Reagent for detecting antibacterial drugs and preparation method thereof

A technology for antibacterial drugs and reagents, which is applied in the field of liquid chromatography tandem mass spectrometry detection to achieve the effects of high responsivity, improved detection efficiency, and improved stability

Active Publication Date: 2021-12-21
江苏豪思睦可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, only voriconazole can be detected by immunoassay

Method used

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  • Reagent for detecting antibacterial drugs and preparation method thereof
  • Reagent for detecting antibacterial drugs and preparation method thereof
  • Reagent for detecting antibacterial drugs and preparation method thereof

Examples

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Effect test

preparation example Construction

[0025] The embodiment of the present invention also provides the preparation method of the reagent for detecting antibacterial drugs described in the previous embodiment, which includes: in the calibrator solution and / or quality control solution for liquid chromatography tandem mass spectrometry detection of antibacterial drugs 3-(N-morpholine)propanesulfonic acid was added respectively, and after the solution was freeze-dried, freeze-dried products corresponding to the calibrator and / or quality control substance were obtained.

[0026] It should be noted that the freeze-drying technique of the solution and the preparation of the calibrator solution and the quality control solution without adding 3-(N-morpholine) propanesulfonic acid can be obtained based on the conventional technical knowledge in the field, and will not be repeated here. , as long as the quality control substance and / or calibrator added with 3-(N-morpholine)propanesulfonic acid is used for antibacterial drug d...

Embodiment 1

[0050] A liquid chromatography tandem mass spectrometry kit for detecting multiple antibacterial drugs, the preparation method of which is as follows.

[0051] Standard products: The standard products of 16 kinds of antibacterial drugs and stable isotope internal standards were all purchased from Beijing Zhenzhen Biological Company, with a purity of ≥99%.

[0052] At the same time, the target linezolid (m / z 338.0→296.0), linezolid internal standard (m / z341.0→297.0), meropenem (m / z 384.0→141.1), meropenem internal standard (m / z z 390.0→147.0), fluconazole (m / z307.0→220.0), fluconazole internal standard (m / z 311.0→223.2), voriconazole (m / z 350.0→280.0), voriconazole internal standard (m / z353 .2→127.1), posaconazole (m / z 701.0→127.0 / 701.0→614.0), posaconazole internal standard (m / z 705.0→127.0), itraconazole (m / z 705.0→392.0 / 705.0→432.0), itraconazole internal standard (m / z714.0→401.0), caspofungin (m / z 547.6→131.0), caspofungin internal standard (m / z 549.5→131.0), moxisar sta...

Embodiment 2

[0074] A method for detecting multiple antibacterial drugs by liquid chromatography tandem mass spectrometry, comprising the following steps.

[0075] 1. Pretreatment of plasma / serum samples:

[0076] Use the protein precipitation method to pre-treat the sample to be tested. Take 100 μL of plasma sample, add 100 μL of internal standard standard to it, add 300 μL of methanol precipitant, after protein precipitation, vortex shaking, take the supernatant for LC-MS / MS analyze.

[0077] 2. Qualitative and quantitative detection:

[0078] The supernatant obtained after processing the sample by protein precipitation is analyzed by liquid chromatography-tandem mass spectrometry (LC-MS / MS). After the analyte is separated by chromatography, it is introduced into the mass spectrometer and ionized in the ion source to form charged ions . Under the action of the electric field, the focus enters the triple quadrupole mass analyzer. In the first quadrupole (Q1), the charged ions are sepa...

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Abstract

The invention discloses a reagent for detecting antibacterial drugs and a preparation method thereof and relates to the technical field of liquid chromatography-tandem mass spectrometry detection. A quality control product and a calibration product are prepared with 3-(N-morpholine) propanesulfonic acid (MOPS) as a stabilizer, stability of the quality control product and the calibration product can be effectively improved, plasma matrix components are kept to the maximum extent, and the transportation and storage processes of calibration products and quality control products are simplified. When the calibrator and the quality control product prepared by the invention are used for liquid chromatography-tandem mass spectrometry detection of antibacterial drugs, detection of at least 16 drugs can be ensured at the same time, detection efficiency is remarkably improved, and the calibrator and the quality control product are high in responsivity and good in repeatability.

Description

technical field [0001] The invention relates to the technical field of liquid chromatography tandem mass spectrometry detection, in particular to a reagent for detecting antibacterial drugs and a preparation method thereof. Background technique [0002] At present, various antibacterial drugs are widely used in clinical practice. Rational use of antibacterial drugs is crucial to improving the clinical efficacy of anti-infection therapy, shortening the treatment time of patients, and reducing medical expenses. Abuse of antimicrobial drugs or illegal addition of antimicrobial drugs in some Chinese patent medicines and cosmetics will lead to the increase of drug-resistant bacteria and the enhancement of bacterial drug resistance, further exacerbating the threat of infectious diseases to human health. [0003] Currently, there are no systematic clinical testing methods for most antibacterial drugs. Therefore, laboratories still need to develop their own methods to provide hosp...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/04G01N30/34
CPCG01N30/02G01N30/04G01N30/34G01N2030/042G01N2030/045
Inventor 肖冰心栗琳王春静张红王翠竹雒琴丁亮周立
Owner 江苏豪思睦可生物科技有限公司
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