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Application of biomarker in preparation or screening of liver cancer diagnostic reagent

A biomarker, a technology for cancer diagnosis, applied in the field of biomarkers in the preparation or screening of diagnostic reagents for liver cancer

Pending Publication Date: 2021-12-28
深圳市龙华区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is a lack of biomarkers and methods for effectively assessing cancer recurrence such as liver cancer

Method used

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  • Application of biomarker in preparation or screening of liver cancer diagnostic reagent
  • Application of biomarker in preparation or screening of liver cancer diagnostic reagent
  • Application of biomarker in preparation or screening of liver cancer diagnostic reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] The kit provided in this embodiment comprises:

[0123] Trizol lysate (Invitrogen, 15596-026);

[0124] RNAse-free homogenizer;

[0125] RNAse-free 1.5ml tube;

[0126] Chloroform;

[0127] isopropanol;

[0128] Washing solution (75% ethanol prepared with RNAse-free water);

[0129] RNAse-free water;

[0130] Reverse transcription reagent mixture (10 μL / tube, TAKARA, RR047A);

[0131] Q-PCR reagent mix (TAKARA, 639676);

[0132] Primer diluent (aqueous solution containing primers).

[0133] The operation method is as follows:

[0134] 1. Tumor sample preparation: For patients with multiple liver cancer foci, a piece of tumor (50 mg / part) was taken from each foci, and a total of 3 foci were taken; for patients with less than 3 liver cancer foci, random Take 3 tumor blocks (50 mg / part); the tumor tissues are labeled as HCC1, HCC2, HCC3. Take 3 pieces of paracancerous tissues randomly (50 mg / part), and mark Nor1, Nor2, Nor3.

[0135] 2. Cut the tissue as small a...

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Abstract

The invention discloses application of a biomarker in preparation or screening of a liver cancer diagnostic reagent. The biomarker comprises at least one of following genes: Anxa1, Anxa4, CD90, Gpc3, Ccnd2 and Afp. The biomarker provided by the invention can effectively predict prognosis of a patient, and provides a basis for further treatment.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of biomarkers in the preparation or screening of diagnostic reagents for liver cancer. Background technique [0002] Cancer is one of the major diseases that threaten human health, and also shortens human life span. For example, liver cancer can cause damage to liver function. Among them, hepatocellular carcinoma (HCC) is one of the top five cancer killers in the world. Hepatectomy is the most commonly used treatment, achieving a 70% 5-year survival rate. Due to the high-malignancy characteristics of liver cancer, its recurrence and metastasis are the main factors affecting the survival of patients after surgery. In the prior art, there is a lack of biomarkers and methods for effectively assessing cancer recurrence such as liver cancer. Contents of the invention [0003] According to the first aspect, in one embodiment, the use of biomarkers in the preparation or screening of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G16H50/30
CPCC12Q1/6886G16H50/30C12Q2600/158C12Q2600/118C12Q2600/136
Inventor 陈协辉赵力敏邓凯陈鹏飞
Owner 深圳市龙华区中心医院
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