Use of erianin in preparing anti-bladder cancer drug for targeted inhibition of AKT activation

An Erianin, bladder cancer technology, applied in the application field of anti-bladder cancer drugs, can solve the problem of less research on anti-bladder cancer cell T24, achieve good in vitro anti-tumor effect, and broaden the effect of medical use

Pending Publication Date: 2022-01-07
广西皓智科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although Erianin, which has less toxic and side effects, has been studied in the inhibition of many cancers, there is less research on the anti-bladder cancer cell T24
At present, whether erianin can effectively target and inhibit the activation of AKT, thereby inhibiting the proliferation of bladder cancer cell T24 is not very clear

Method used

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  • Use of erianin in preparing anti-bladder cancer drug for targeted inhibition of AKT activation
  • Use of erianin in preparing anti-bladder cancer drug for targeted inhibition of AKT activation
  • Use of erianin in preparing anti-bladder cancer drug for targeted inhibition of AKT activation

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Experimental program
Comparison scheme
Effect test

Embodiment

[0030] 1. Materials and methods

[0031] 1. Cell lines.

[0032] Human bladder cancer T24 cell line was purchased from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences.

[0033] 2. Drugs and reagents.

[0034] Erianin, the chemical name is 2-methoxy-5-[2-(3,4,5-trimethoxy-phenyl)-ethyl]-phenol, the chemical structure is as follows figure 1 As shown, purchased from Shanghai Yuanye Biotechnology Co., Ltd., article number: B20844, CAS number: 95041-90-0.

[0035] DMSO reagent is used for dissolving erianin, after dissolving, filtering and sterilizing, store at room temperature in the dark for future use.

[0036] All reagents are commercially available as retail items.

[0037] 3. Main instruments.

[0038] 311 air jacket CO 2 Incubator, Thermo Fisher Scientific, USA; Infinite M200 proNano Quant grating type continuous wavelength microplate reader, TECAN, Switzerland; Elix3+30L+3YNERGY ultrapure water system, Millipore, USA;...

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Abstract

The invention provides use of erianin in preparing an anti-bladder cancer drug for targeted inhibition of AKT activation. The erianin reduces expressions of p-Akt and AKT, up-regulates expressions of cell cycle-related proteins P27 and P21 at the same time, enables a cell cycle to retard at a G1 phase, and thus inhibits proliferation of bladder cancer T24 cells. It is found in tests that erianin has an inhibiting effect on bladder cancer T24 cells and shows good time and concentration dependence, a theoretical basis is provided for further research of action mechanism of the erianin on bladder cancer, a new treatment scheme is provided for treatment of bladder cancer, and a new candidate drug is provided for treatment of bladder cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of erianin in the preparation of an anti-bladder cancer drug targeting to inhibit AKT activation. Background technique [0002] AKT, also known as protein kinase B (protein kinase B, PKB), is a member of the serine / threonine protein kinase family discovered in 1991. It exists in mammals and is translated into three isoforms by three different genes: Akt1, Akt2 and Akt3, play a crucial role in controlling cell survival and apoptosis. In addition to cell cycle regulation, apoptosis initiation and other cell survival regulation, its function also participates in many important physiological and pathological processes such as angiogenesis, telomerase activity and cell invasion. Current studies have found that AKT is an important signaling molecule for tumorigenesis. Its subtype Akt2 is a protein composed of 481 amino acids. Promote cell proliferation and inhib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/09A61K36/8984A61P35/00
CPCA61K31/09A61K36/8984A61P35/00
Inventor 谭宁陈筠李浩周萍曾长春薛宇杨凤娟周程
Owner 广西皓智科技有限公司
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