Composition, kit and method for detecting pathogens causing respiratory tract infection and identifying pathogen species and application

A respiratory and composition technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex pathogens, poor detection rate and accuracy, achieve high throughput and avoid false positives and environmental pollution, reducing the effect of psychological burden

Active Publication Date: 2022-01-18
SANSURE BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies still face the situation that the detection rate and accuracy are not good because the pathogens to be detected are too complex

Method used

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  • Composition, kit and method for detecting pathogens causing respiratory tract infection and identifying pathogen species and application
  • Composition, kit and method for detecting pathogens causing respiratory tract infection and identifying pathogen species and application
  • Composition, kit and method for detecting pathogens causing respiratory tract infection and identifying pathogen species and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, primers and probes used in the present invention

[0065] The primers and probes used in the present invention are shown in Table 1 below (SEQ ID NO:1~24 only corresponds to the base sequence, and the labeling method here is only a specific example):

[0066] Table 1

[0067]

[0068]

Embodiment 2

[0069] Embodiment 2, detect the pathogen that causes respiratory tract infection and identify the method for the type of pathogen

[0070] The detection samples of the present invention are throat swabs, sputum, alveolar lavage fluid, and blood. The magnetic bead method is used to extract viral nucleic acid, and the following operations are performed in the sample processing room:

[0071] 2.1 Take an appropriate amount of 1.5mL sterilized centrifuge tubes, mark the negative control, positive control and samples to be tested respectively, and add 300 μL RNA extraction solution 1 to each tube;

[0072] 2.2 Add 200 μL of the sample to be tested or negative control or positive control to each tube; cover the tube cap, vortex and mix for 10 seconds, and centrifuge briefly;

[0073] 2.3 Add 100 μL RNA Extraction Solution 2-mix to each tube (mix well and draw), shake and mix for 10 seconds, then let stand at room temperature for 10 minutes;

[0074] 2.4 Place the centrifuge tube o...

Embodiment 3

[0094] Embodiment 3, the detection rate of composition of the present invention

[0095] Use the compositions in Table 1 of the present invention to detect according to the method described in Example 2, and design a series of controls in addition (other dual-probe schemes: probe 1: CY5-CTAACCTTAACCCCGCAGC-BHQ2; probe 2: CY5-AATCCTAACCATGGAGCAAG- BHQ2) is also detected. The test results are shown in Table 2 and Table 3.

[0096] Table 2. Statistical table of Ct value results of different rhinovirus detection schemes

[0097]

[0098] Note: The larger the Ct value, the worse the detection effect, and No Ct means negative.

[0099] It can be seen from the test results in the above table that enteroviruses (Coxsackievirus EV71, Coxsackievirus A6) will not be detected by using this patented solution for detection; at the same time, for rhinovirus positive samples, using this patented solution for detection, its Ct value Both are small, indicating that its detection effect is...

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PUM

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Abstract

The invention belongs to the field of molecular biological detection. Specifically, the invention belongs to the field of detection of pathogens causing respiratory tract infection; and more specifically, the present invention can simultaneously detect influenza A virus, influenza B virus, rhinovirus, respiratory adenovirus, respiratory syncytial virus, and mycoplasma pneumoniae. By using a composition provided by the invention, the six pathogens causing respiratory tract infection can be detected at the same time, and specific infection of one or more pathogens can be identified. Meanwhile, the detection rate is higher than that of the existing composition, and the detection is more accurate.

Description

technical field [0001] The invention belongs to the field of molecular biology detection; specifically, it belongs to the field of detection of pathogens causing respiratory infections; more specifically, the invention can simultaneously detect influenza A virus, influenza B virus, rhinovirus, respiratory adenovirus, respiratory tract infection Cytovirus and Mycoplasma pneumoniae. Background technique [0002] Most respiratory infectious diseases are clinically common and frequently-occurring diseases. These diseases have similar clinical symptoms and epidemic characteristics, and it is difficult to identify and determine the type of pathogen infected by clinical symptoms and routine laboratory tests. Respiratory pathogens can be transmitted through the air. The pathogens that cause acute respiratory diseases have the characteristics of strong infectivity, rapid transmission, short incubation period, and acute onset. They can cause a wide range of acute upper and lower resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/689C12Q1/686C12N15/11C12R1/35
CPCC12Q1/701C12Q1/689C12Q1/686C12Q2563/107C12Q1/70C12Q1/702C12Q2600/16
Inventor 陈晓亮任小梅邓中平刘佳戴立忠
Owner SANSURE BIOTECH INC
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