Breeding method of good young triploid oysters
A technology of triploid and oyster seedlings, applied in fish farming, application, climate change adaptation, etc., can solve problems affecting the quality and quality of triploid oyster seedlings, slow growth of oysters, low glycogen content, etc., to achieve The effect of high oyster yield, strong manipulability, and high glycogen content
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Embodiment 1
[0038] Please refer to figure 1 Shown, a kind of good oyster seedling breeding method of triploid oyster comprises the following steps:
[0039] Step 1. Selecting parents: select the shell bodies with mature glands as parents.
[0040] The method of selecting glandular mature shell bodies as parents is as follows:
[0041] Select the shellfish with a shell length of 8 cm and grow well in the first instar shellfish; clean and disinfect the shellfish, put them into a net dragon and hang them in the broodstock breeding pond for storage; check the maturity of the gonads for each shellfish.
[0042] When stocked in broodstock breeding ponds, the density is 40 / m 3 , keep the water temperature at 20°C.
[0043] Step 2. Squeeze fertilized eggs: Selectively glandular-matured parent shellfish are dissected to obtain fertilized eggs. After the number of eggs reaches the requirement, they are ripened in vitro, so that the blastocysts in the eggs disappear, and fertilization is carried ...
Embodiment 2
[0054] A kind of oyster triploid good oyster seedling breeding method provided by the present embodiment is roughly the same as embodiment 1, and its main difference is:
[0055] In step 1, the shell length of the shellfish is 9cm, and the 1st instar shellfish that has grown is selected. When it is stocked in the shellfish cultivation pond, the density is 45 / m 3 , keep the water temperature at 24°C.
[0056] In step 2, the in vitro maturation time is 45 minutes. During fertilization, the water temperature is controlled at 24°C. After 45% of the first polar body appears, colchicine is added and poured into the pool after 17 minutes.
[0057] In step 3, larvae are cultivated: the cultivation density is 6 larvae / ml.
[0058] In step 4, when the length of the larval shell is 305 μm, start to put in the attachment base, and each family casts 100 pieces. After 5 days after the attachment base is put in, each attachment base is attached to 25 juvenile shells, and the temperature beg...
Embodiment 3
[0061] A kind of oyster triploid good oyster seedling breeding method provided by the present embodiment is roughly the same as embodiment 1, and its main difference is:
[0062] In step 1, the shell length of the shellfish is 9cm, and the 1st instar shellfish that has grown is selected. When it is stocked in the shellfish cultivation pond, the density is 50 / m 3 , keep the water temperature at 28°C.
[0063] In step 2, the in vitro maturation time is 60 minutes. During fertilization, the water temperature is controlled at 25°C. After 50% of the first polar body appears, colchicine is added and poured into the pool after 20 minutes.
[0064] In step three, larvae are cultivated: the cultivation density is 8 larvae / ml.
[0065] In step 4, when the length of the larval shell is 340 μm, start to put in the attachment base, and each family casts 100 pieces. After 5 days after the attachment base is put in, each attachment base is attached to 30 juvenile shells, and the temperature...
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