Breeding method of good young triploid oysters

A technology of triploid and oyster seedlings, applied in fish farming, application, climate change adaptation, etc., can solve problems affecting the quality and quality of triploid oyster seedlings, slow growth of oysters, low glycogen content, etc., to achieve The effect of high oyster yield, strong manipulability, and high glycogen content

Pending Publication Date: 2022-01-21
粤源种质科技(湛江)有限公司
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Problems solved by technology

[0004] Chinese patent application number CN201010595675.7 discloses a method for cultivating triploid single oysters. Oyster production, ploidy detection and other steps, the combination of triploid technology and individual oyster seed breeding technology to cultivate new oyster varieties, and at the same time can be produced on a large scale. Regular, beautiful, deep shell and fat meat, etc.”, although the triploid oyster seedlings bred in the above patent documents have a certain glycogen content, but the glycogen content is not high, and at the same time, the production cost is high when breeding. , oyster growth rate is slow, oyster yield is low, which affects the quality and quality of the triploid oyster seedlings bred

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  • Breeding method of good young triploid oysters
  • Breeding method of good young triploid oysters

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Please refer to figure 1 Shown, a kind of good oyster seedling breeding method of triploid oyster comprises the following steps:

[0039] Step 1. Selecting parents: select the shell bodies with mature glands as parents.

[0040] The method of selecting glandular mature shell bodies as parents is as follows:

[0041] Select the shellfish with a shell length of 8 cm and grow well in the first instar shellfish; clean and disinfect the shellfish, put them into a net dragon and hang them in the broodstock breeding pond for storage; check the maturity of the gonads for each shellfish.

[0042] When stocked in broodstock breeding ponds, the density is 40 / m 3 , keep the water temperature at 20°C.

[0043] Step 2. Squeeze fertilized eggs: Selectively glandular-matured parent shellfish are dissected to obtain fertilized eggs. After the number of eggs reaches the requirement, they are ripened in vitro, so that the blastocysts in the eggs disappear, and fertilization is carried ...

Embodiment 2

[0054] A kind of oyster triploid good oyster seedling breeding method provided by the present embodiment is roughly the same as embodiment 1, and its main difference is:

[0055] In step 1, the shell length of the shellfish is 9cm, and the 1st instar shellfish that has grown is selected. When it is stocked in the shellfish cultivation pond, the density is 45 / m 3 , keep the water temperature at 24°C.

[0056] In step 2, the in vitro maturation time is 45 minutes. During fertilization, the water temperature is controlled at 24°C. After 45% of the first polar body appears, colchicine is added and poured into the pool after 17 minutes.

[0057] In step 3, larvae are cultivated: the cultivation density is 6 larvae / ml.

[0058] In step 4, when the length of the larval shell is 305 μm, start to put in the attachment base, and each family casts 100 pieces. After 5 days after the attachment base is put in, each attachment base is attached to 25 juvenile shells, and the temperature beg...

Embodiment 3

[0061] A kind of oyster triploid good oyster seedling breeding method provided by the present embodiment is roughly the same as embodiment 1, and its main difference is:

[0062] In step 1, the shell length of the shellfish is 9cm, and the 1st instar shellfish that has grown is selected. When it is stocked in the shellfish cultivation pond, the density is 50 / m 3 , keep the water temperature at 28°C.

[0063] In step 2, the in vitro maturation time is 60 minutes. During fertilization, the water temperature is controlled at 25°C. After 50% of the first polar body appears, colchicine is added and poured into the pool after 20 minutes.

[0064] In step three, larvae are cultivated: the cultivation density is 8 larvae / ml.

[0065] In step 4, when the length of the larval shell is 340 μm, start to put in the attachment base, and each family casts 100 pieces. After 5 days after the attachment base is put in, each attachment base is attached to 30 juvenile shells, and the temperature...

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Abstract

The invention relates to the technical field of oyster breeding, in particular to a breeding method of good young triploid oysters. The breeding method comprises the following steps: S1, parent selection, S2, sperm and egg extraction, S3, larva breeding, S4, adherance putting, S5, mariculture and S6, detection. The triploid is high in glycogen content and good in quality and taste, marine buoyant raft culture is adopted, and compared with a traditional mode, the production cost is low, management is easy, the oyster growth speed is high, the individual shell type is regular, the hemps are uniform in size, manual operability is high, the oyster yield is high, products are not prone to being lost, the characteristics determine that the triploid oysters cultured by the method have conditions to enter domestic and international high-grade oyster markets to replace and improve the existing oyster cultivation varieties, so that the huge domestic oyster cultivation industry is really driven to realize industrial upgrading.

Description

technical field [0001] The invention relates to the technical field of oyster reproduction, in particular to a method for breeding oyster triploid good oyster seedlings. Background technique [0002] Oysters, commonly known as sea oysters, oysters (oysters), oysters, oysters, etc., are widely distributed bivalve molluscs. More than 100 species have been discovered so far. They are produced in almost all coastal countries in the world, and their output ranks first among shellfish. . China is rich in oyster resources and has a long history of exploiting this resource. The ancestors in some areas along the southern coast have mastered the technique of planting bamboo to raise oysters as early as the Han Dynasty, which has a history of more than 2,000 years. Since the founding of the People's Republic of China, especially since the implementation of the reform and opening up policy, China's oyster production has continued to grow. After entering the 21st century, it has shown ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54A01K67/033
CPCA01K61/54A01K67/0334A01K2227/70Y02A40/81
Inventor 吴思远曾凤仙
Owner 粤源种质科技(湛江)有限公司
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