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Reconstruction of soft tissue-bone immune repair environment periosteum-bone complex and preparation method

An immune repair and complex technology, applied in tissue regeneration, prosthetics, medical science, etc., can solve the problem of no periosteum-bone complex, achieve coordination of early immune regulation of osteogenesis-angiogenesis events, and promote bone repair , The effect of low cytotoxicity of the scaffold

Active Publication Date: 2022-07-22
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no reports on the preparation of periosteum-bone complex

Method used

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  • Reconstruction of soft tissue-bone immune repair environment periosteum-bone complex and preparation method
  • Reconstruction of soft tissue-bone immune repair environment periosteum-bone complex and preparation method
  • Reconstruction of soft tissue-bone immune repair environment periosteum-bone complex and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Periosteum-bone complex aimed at reconstructing soft tissue-bone immune repair environment and its preparation method

[0045] (1) Take material

[0046] The femurs of healthy adult large white pigs were taken within 12 hours of slaughter, the metaphysis was separated, the femoral shaft was collected, the internal marrow cavity was removed, and the femoral shaft was divided into 10mm*8mm pieces;

[0047] (2) Preprocessing

[0048] Take a certain amount of flaky bone, place it on a low-temperature (4°C) shaker and shake at 100 rpm, and rinse it with PBS buffer containing protease inhibitors for 3 times for 10 min each time to remove blood, attached fat fragments and other impurities;

[0049] (3) Decalcification

[0050] The pretreated flaky bones were placed in a 20% (w / v) EDTA-2Na solution for ultrasonic decalcification for 12 days, the ultrasonic parameters were 40kHZ, and the temperature was 22°C;

[0051] (4) Obtaining the periosteum-bone complex

[0...

example 1

[0063] 1. In Example 1, the macroscopic view and decellularization assessment of the periosteum-bone complex scaffold

[0064] The general appearance and CT scan of the periosteum-bone complex scaffold, such as figure 1 .

[0065] 2. In Example 1, Decellularization Assessment of Periosteum-Bone Complex Scaffolds

[0066] H&E staining showed that no cells and no nuclear components remained, and DNA quantitative detection almost did not contain DNA components, such as figure 2 .

[0067] 3. In Example 1, Microstructural Evaluation of Periosteum-Bone Complex Scaffolds

[0068] Scanning electron microscope was used to observe collagen arrangement, bone crystal structure and periosteum-bone interface connection structure of the present invention, such as image 3 .

[0069] 4. Surface topography and mechanical evaluation of the periosteum-bone complex scaffold in Example 1

[0070] Atomic force microscope observes the microscopic surface morphology of the present invention, ...

Embodiment 2

[0081] Example 2: Periosteum-bone complex aimed at reconstructing soft tissue-bone immune repair environment and its preparation method

[0082] (1) Take material

[0083] The femurs of healthy adult large white pigs were taken within 12 hours of slaughter, the metaphysis was separated, the femoral shaft was collected, the internal marrow cavity was removed, and the femoral shaft was divided into 10mm*8mm pieces;

[0084] (2) Preprocessing

[0085] Take a certain amount of flaky bone, place it on a low-temperature (4°C) shaker and shake at 100 rpm, and rinse it with PBS buffer containing protease inhibitors for 3 times for 10 min each time to remove blood, attached fat fragments and other impurities;

[0086] (3) Decalcification

[0087] The pretreated flaky bones were placed in a 20% (w / v) EDTA-2Na solution for ultrasonic decalcification for 12 days, the ultrasonic parameters were 40kHZ, and the temperature was 22°C;

[0088] (4) Obtaining the periosteum-bone complex

[0...

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Abstract

The invention discloses a periosteum-bone complex for reconstructing soft tissue-bone immune repair environment and a preparation method. After cutting and proofing, repeatedly rinsed with deionized water, liquid nitrogen freeze-thaw, ultrasonic decalcification, PBS buffer containing protease inhibitors, PBS buffer containing Triton X-100, PBS buffer containing SLES, PBS buffer containing DNase I solution and Tris-HCl buffer to obtain acellular periosteum-bone composite material. The complex retains the good spatial structure of periosteum-bone and extracellular active components, and provides a biphasic interface of "perichondrium-durabone", which effectively blocks excessive inflammatory infiltration from soft tissue sources and participates in the early immune remodeling of soft tissue-bone injury. , combined with its own advantages of osteogenesis and angiogenesis, to form a benign immune-repair microenvironment, providing new ideas for biomaterials to promote bone tissue regeneration and repair.

Description

technical field [0001] The invention mainly relates to the biological field for regeneration and repair of bone tissue, in particular to a periosteum-bone complex aiming at rebuilding a soft tissue-bone immune repair environment and a preparation method thereof. Influence of bone repair, rational use of the advantages of space composite mode scaffold to promote bone regeneration and repair. Background technique [0002] Bone defects caused by trauma, tumors and infections are common clinical diseases, affecting human health, especially multiple complex bone defects, which often require repeated treatment, causing heavy psychological pressure and economic burden on individuals, and social stability. The potential impact is an urgent problem to be solved in clinical orthopaedics. [0003] In recent years, the replacement therapy of biomaterials has gradually attracted people's attention. Among them, single-phase bone substitutes with high stiffness (such as bioceramics, biogl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/50A61L2430/02A61L2430/40A61L2430/34
Inventor 林贤丰赵晨晨范顺武
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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