Kit for detecting eight respiratory pathogens including novel coronavirus and application thereof

Abstract

The invention discloses a kit for detecting eight respiratory pathogens including novel coronavirus and application thereof. The kit comprises specific amplification primers for amplifying nine gene loci of the following eight respiratory pathogens, wherein the nine gene loci are ORF and N genes of a 2019 novel coronavirus, a hemagglutinin gene of an influenza A virus, an M protein gene of an influenza B virus, an upstream segment of a Hexon gene Loop1 of an adenovirus, a hemagglutinin-neuraminidase gene of a parainfluenza virus, an F protein gene of a respiratory syncytial virus, a VP1 capsid protein gene of a rhinovirus and a 16S rRNA gene of mycoplasma pneumoniae; and a specific amplification primer of the reference gene ACTB. According to the kit, a multiple RT-qPCR technology is adopted, highly conserved sequences of a human reference gene, a novel coronavirus SARS-CoV-2 and other seven common respiratory viruses are taken as target regions, nine groups of specific primers and probes are designed, a three-tube quadruple detection system is constructed, and higher specificity is achieved.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and relates to a detection kit covering novel coronavirus and 7 other respiratory pathogens, specifically a detection kit for 8 respiratory pathogens containing new crown and its application. Background technique [0002] The initial symptoms of the new coronavirus (SARS-CoV-2) infection mainly include fever, general pain, fatigue, etc. The symptoms of parainfluenza virus (HPIV) and respiratory syncytial virus (HRSV) are similar. [0003] The current detection methods for diagnosing respiratory virus infection in my country mainly include: 1) culture method; 2) mass spectrometry; 3) immunological detection; 4) PCR method and so on. Among them, obtaining living organisms of pathogens (for bacteria, fungi, and viruses, mainly culture-positive) is the gold standard for the diagnosis of infectious diseases. However, in vitro culture often shows negative results in the early stage of inf...

AI Technical Summary

Problems solved by technology

[0004] At present, there are few kits for multiple detection of respiratory viruses in China. Boao Jingxin eight-item respiratory virus nucleic acid detection kit (constant temperature amplification chip method), Zhongzhi Bio three-item respiratory virus nucleic acid detection kit (double amplification method) and seven nucleic acid detection kits for respiratory pathogens have been approved for marketing, but none of the above products contain detection targets for the new coronavirus (SARS-CoV-2)

In foreign countries, there are no nucleic acid detection kits for the simultaneous detection of the new coronavirus SARS-CoV-2 and other different respiratory viruses.

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