Quantitative detection method for heavy metal cadmium ions

A quantitative detection method and quantitative detection technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve problems such as inability to perform quantitative detection

Pending Publication Date: 2022-02-11
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The results of immunogold standard color test cards are usually observed by naked eyes, which can only be qualitative or semi-quantitative

Method used

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  • Quantitative detection method for heavy metal cadmium ions
  • Quantitative detection method for heavy metal cadmium ions

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Experimental program
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Embodiment 1

[0051] The present invention provides such Figure 1-2 A quantitative detection method for heavy metal cadmium ions shown, comprising the steps:

[0052] S1. Prepare a cadmium ion immune gold label quick test card. The preparation of the cadmium ion immune gold label quick test card includes the following steps:

[0053](1) Preparation of nitrocellulose membrane containing detection line T and quality control line C, the preparation of nitrocellulose membrane containing detection line T and quality control line C includes preparation of coated antigen and coating of nitrocellulose membrane, wherein The preparation of the coated antigen comprises the following steps: the cadmium ion coated antigen is diluted to 0.25mg / mL with 10mM PBS buffer solution with a pH value of 7.4 to obtain the cadmium ion coated antigen solution, which is used as the coated antigen of the detection line T; The anti-mouse IgG secondary antibody solution is a goat anti-mouse IgG secondary antibody dilu...

Embodiment 2

[0075] Please refer to figure 2 , the present invention also discloses an immunogold standard rapid test card for detecting cadmium ions, an immune gold standard rapid test card, comprising a base plate 2 and a sample pad 6 placed thereon, coated with colloidal gold-labeled cadmium ion mouse monoclonal antibody The gold standard pad 5, the nitrocellulose membrane containing the test line T and the quality control line C 3, and the absorbent pad 4, the test line T is coated with cadmium ion-coated antigen, and the quality control line C is coated with goat anti-mouse IgG Secondary antibody; the overall exterior of the detection card is wrapped with a casing 1, the casing 1 is provided with a sample injection hole 7 corresponding to the sample pad 6, and the casing 1 is provided with an observation hole 8 corresponding to the quality control line and the detection line.

[0076] In the above quick test card, the gold standard pad 5 coated with colloidal gold-labeled cadmium ion...

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Abstract

The invention discloses a quantitative detection method for heavy metal cadmium ions, and belongs to the technical field of biological detection. The quantitative detection method comprises the following steps: S1, preparing a cadmium ion immunogold-labeled rapid detection card, specifically, wherein preparation of the cadmium ion immunogold-labeled rapid detection card comprises the following steps: (1), preparing a nitrocellulose membrane containing a detection line T and a quality control line C; (2) preparing a gold-labeled pad; (3) assembling an immunogold-labeled rapid test card; and S2, setting a standard curve for quantitative detection of a cadmium ion immunogold-labeled rapid detection card; S3, quantitatively detecting a sample by using the cadmium ion immunogold-labeled rapid detection card. According to the present invention, the immunogold labeling rapid detection card is prepared by using the gold labeling technology, combining the antigen-antibody immunoreaction and using the lateral chromatography principle, can be used for rapid immunodetection, and can be combined with the color generation analyzer, the color generation analyzer determines the depth of the C line and the T line, the T/C is calculated, and then linear fitting is carried out, and the corresponding parameters is set in the color generation analyzer, the color generation analyzer is applied, and a standard curve is set to realize quantitative detection.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and more specifically relates to a quantitative detection method for heavy metal cadmium ions. Background technique [0002] Cadmium, heavy non-ferrous metal element, chemical symbol Cd, atomic number 48, discovered in 1817, elemental silver-white metal. Cadmium is highly toxic, and the air and food polluted by cadmium are seriously harmful to the human body, and the metabolism in the human body is slow. Cadmium can irritate the respiratory tract. Long-term exposure can cause anosmia, macular spots or yellow circles on the gums. Cadmium compounds are not easily absorbed by the intestines, but can be absorbed by the body through breathing and accumulate in the liver or kidneys, causing harm, especially for Kidney damage is most pronounced and can lead to osteoporosis and softening of the bones. The European Union lists cadmium as a highly hazardous toxic substance and a carcinogen a...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/548G01N33/58G01N33/53G01N33/532B22F9/24
CPCG01N33/577G01N33/558G01N33/548G01N33/587G01N33/5308G01N33/532B22F9/24
Inventor 姚春霞王红梅周佳欣杨海锋
Owner SHANGHAI ACAD OF AGRI SCI
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