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Paraffin-embedded tissue sample processing method and kit for tumor prognosis evaluation

A paraffin-embedded, prognostic assessment technology, applied in the field of pathological diagnosis, can solve problems such as different risk levels and cannot be dynamically explained, and achieve the effect of improving accuracy, efficiency and accuracy

Pending Publication Date: 2022-02-22
MY BIOMED TECH (GUANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that DNA ploidy is associated with the prognosis of patients. The existing DNA ploidy analysis methods are mainly performed by flow cytometry, but the quantification of DNA ploidy obtained by flow cytometry is only a quantitative result. No explanation can be given for the cause of the dynamics, nor can it be further explained that the same multiple risk may be different

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  • Paraffin-embedded tissue sample processing method and kit for tumor prognosis evaluation
  • Paraffin-embedded tissue sample processing method and kit for tumor prognosis evaluation
  • Paraffin-embedded tissue sample processing method and kit for tumor prognosis evaluation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Making the flow of cell nuclear monolays in a variety of paraffin-embedded tissue samples

[0070] The production process of the nuclear monocyclic dyeing smear in this embodiment is like figure 1 As shown, the specifically can include the following steps:

[0071] The tissue sections of tumor enrichment 50 μm thickness were obtained from paraffin-embedded tumor tissue samples, and after dewaxing, rinsing, digestion, screen filtration, centrifugal, filtration, and preparing nuclear single layers;

[0072] The nucleus is hydrolyzed in the nucleus of the nucleus, and then the nucleus is soaked with the nucleus and eluted with the eluate, and then dehydrated, encapsulated, and the dyed nuclear monochride smear was obtained.

Embodiment 2

[0073] Example 2 Extraction of Sample Calcotids of Paraffled Buried Tissue

[0074] This embodiment uses a kit or a nuclear extracting kit for a paraffin-embedded tissue sample treatment method for tumor prognosis, which contains at least:

[0075]

[0076] The amount of the above reagents can be extracted from 5 sample extraction. The kit can be transported at 2-8 ° C, and the lysase is stored in -20 ± 5 ° C, and other reagents are stored at room temperature, and the effective phase of the kit is 18 months.

[0077] The kit may also include a supplies: 5 nylon cell sieve (aperture 60 μm), 5 sheets of filter paper (aperture diameter, 10 magnetic stirrer, 2 black paper jam, 5 boron silicon small glass bottles (10ml), 5, 10 pieces of boron silicon slides, centrifuge (15 ml).

[0078] The steps of nuclear extraction are as follows:

[0079] 2.1 Slices

[0080] 2 tablets of 50 μm thick paraffin bury tissue sections, the slice is not too thin or too thick, the thin fragment is large,...

Embodiment 3

[0105] Example 3 Cell nuclear monochacolate DNA dyeing

[0106] This embodiment uses a kit or a nuclear monoqueric smear DNA dyeing kit for a paraffin coated tissue sample treatment method for tumor prognosis, which contains at least:

[0107]

[0108] The amount of the above reagents can be stained with 5 pieces of smear. The kit can be transported at 2-8 ° C, and the DNA dyed liquid is preserved at 2-8 ° C, and other reagents are stored at normal temperature, and the effective phase of the kit is 18 months.

[0109] The kit can also include a reagent: 70% ethanol solution, 95% ethanol solution, anhydrous ethanol, xylene, Eukitt sealing agent.

[0110] The kit can also include a consumable: tin paper, 1 size of 30 cm × 30 cm, and the other 5 is 7 cm × 7 cm.

[0111] The steps of nuclear staining are as follows:

[0112] 3.1 Acid hydrolysis

[0113] 3.1.1 Transfer the nuclear single-layer slide of the previously fixed liquid to the glass dyeing tank, slowly rinse with the tap wa...

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Abstract

The invention discloses a paraffin-embedded tissue sample processing method and a kit for tumor prognosis evaluation. The method comprises the following steps: obtaining a cell nucleus monolayer from a paraffin-embedded tumor tissue sample, dyeing the cell nucleus by using a dyeing reagent, and collecting an image by using a digital pathological scanning analysis system. The content of cell DNA in the abnormal tissue is detected through image analysis of the gray scale and the IOD value of the cell nucleus, and the DNA ploidy is reflected; through image analysis of cell nucleus textures, chromatin structures are quantified, and the structures comprehensively reflect gene changes and epigenetics changes. According to the method, the combined quantitative analysis of two prognosis risk factors is realized on the paraffin-embedded tissue sample through one-time sampling and one-time processing flow by utilizing a digital pathological scanning analysis system, and the analysis result can be used for prognosis evaluation of a patient, so that the efficiency and the accuracy of evaluation work are greatly improved, and reasonable medication is guided.

Description

Technical field [0001] The present invention belongs to the field of pathological diagnosis, and more particularly to a paraffin embedded tissue sample treatment method for tumor prognosis and a kit thereof. Background technique [0002] Tumors are heterogeneous, and the degree of malignancy in tumor patients is different. Even after the tumor surgery is removed, the patient's prognosis also has different differences, and the chemotherapy drug and treatment of different hazards are different. The post-review frequency requirements are also different, and some drugs are unnecessary for low-risk patients, even invalid or harmful. In order to improve the treatment effect of the tumor, it improves the quality of life, reducing treatment costs, and requires precise medication, which requires prognostic evaluation of tumor patients, and hierarchically. [0003] Tumor samples detected by prognostic evaluation are preferred, easy to obtain. Samples of paraffin-embedded tissue samples are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N1/30G01N1/34G01N21/84
CPCG01N1/36G01N1/30G01N1/34G01N21/84G01N2001/302
Inventor 童艳铮董跃进孙延红李霖陆佳益
Owner MY BIOMED TECH (GUANGZHOU) CO LTD
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