Preparation method and application of reconfigurable uterus derived material

A technology for uterine and uterine exfoliation, which is applied in the field of preparation of uterine-derived materials, can solve the problems of ineffective uterine recovery, low pregnancy rate and live birth rate, difficult endometrial cavity repair, etc., so as to promote wound healing and promote uterine The effect of endometrial regeneration and uterine recovery

Inactive Publication Date: 2022-03-01
龚玉兰
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When these inelastic ECM scaffolds are grafted onto the defective uterine wall, their poor remodeling makes it difficult to penetrate deep into...

Method used

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  • Preparation method and application of reconfigurable uterus derived material
  • Preparation method and application of reconfigurable uterus derived material
  • Preparation method and application of reconfigurable uterus derived material

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Embodiment Construction

[0034] A method for preparing a reconfigurable uterus-derived material, comprising the steps of:

[0035] (1) Extraction of uterine decellularized extracellular matrix hydrogel:

[0036] (I) Decellularization: Cut the uterus (3 uteri selected from pigs, wash with deionized water, transfer to the laboratory, and store at -20°C) into slices (about 2cm×1cm×0.5cm), add Add it to 10 mL of an aqueous solution containing 0.02% trypsin and 0.05% EDTA, stir at 37°C for 4 hours; wash the uterine slices with deionized water three times, add to another aqueous solution containing 3% Triton x-100, and stir slowly under a stirrer at room temperature 4h, after washing with deionized water for 60min, slowly stir the uterine slices on a stirrer for 4h (4% sodium deoxycholate solution), wash again with deionized water for 60min, soak in deionized water for 3h, and obtain white porous uterine stent;

[0037] (II) Disinfection: Soak the uterine stent in 75% ethanol, wash it with deionized water...

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Abstract

The invention discloses a preparation method and application of reconfigurable uterus derived materials (RUMs), and the preparation method comprises the following construction steps: (1) extraction, sterilization and identification of pig uterus acellular matrix hydrogel, and (2) preparation of the RUMs. The preparation method comprises the following construction steps: (1) preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs, preparation of the RUMs and preparation of the RUMs. (2) combining the extracellular matrix (ECM) hydrogel derived from the uterus with chorionic mesenchymal stem cells (MSCs), and culturing to construct RUMs gel and RUMs patches; (3) using the RUMs to treat the serious uterus injury of the rat, and verifying the curative effect. A serum-free and heterologous culture medium is used in the whole research process, the GMP standard is met, immune response is not caused, the cost is low, the source is wide, large-scale batch production can be achieved, the system is mature and easy to copy, the effect is stable, and a new treatment scheme is provided for treating human uterus factor infertility and uterus injuries caused by various operations.

Description

technical field [0001] The invention belongs to the technical field of transplantation of stem cells combined with decellularized biological materials, and in particular relates to a preparation method and application of a reconfigurable uterus-derived material. Background technique [0002] Infertility has received great attention in today's society and has become an increasingly serious public health problem. Uterine factor infertility due to endometrial damage is becoming more common, with approximately 6% of women undergoing uterine repair. Some clinical strategies such as hysteroscopic transadhesectomy, physical barriers (contraceptive device, intrauterine balloon, etc.), and hormonal drugs have been developed to repair the damaged uterus. However, these strategies are difficult to effectively repair the uterus and restore fertility, and further cause irreversible infertility due to intrauterine adhesions. Therefore, new effective solutions are urgently needed to rege...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/52
CPCA61L27/3604A61L27/3679A61L27/3633A61L27/3687A61L27/3691A61L27/52A61L27/50A61L2400/06
Inventor 龚玉兰
Owner 龚玉兰
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