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Cardiovascular implant intervention material/instrument biological film coating and preparation method thereof

A cardiovascular and coating technology, applied in the field of cardiovascular implant materials/device bio-coating coatings and its preparation, can solve the problems of inability to apply large-scale medical devices, strong immune rejection, poor blood compatibility, etc., to achieve Superior clinical application prospects, favorable for mass production, and mild preparation conditions

Active Publication Date: 2022-03-01
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the application of cell membranes is currently limited to the coating of nanomaterials and cannot be applied to large-scale medical devices.
At present, cardiovascular interventional devices such as stents, artificial heart valves, and occluders still face problems such as poor blood compatibility, poor biological activity, and strong immune rejection, which lead to various clinical complications.

Method used

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  • Cardiovascular implant intervention material/instrument biological film coating and preparation method thereof
  • Cardiovascular implant intervention material/instrument biological film coating and preparation method thereof
  • Cardiovascular implant intervention material/instrument biological film coating and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A kind of cardiovascular implant intervention material / apparatus bio-film coating, its preparation method comprises the following steps:

[0034] (1) Centrifuge whole blood to obtain platelets, then resuspend them in PBS solution containing 1mM EDTA, then add protease inhibitors and freeze-thaw 5 times repeatedly, then wash, resuspend and sonicate in sequence to obtain platelet membrane vesicle suspension ; The protein concentration of the cell membrane vesicle suspension is 2mg / mL;

[0035] (2) Place the polylactic acid material in a sodium acetate solution containing 2mg / mL dopamine hydrochloride and 2mg / mL sodium periodate, react at a temperature of 30°C for 25min, wash with deionized water, and repeat this 3 times to obtain superhydrophilic coating;

[0036] (3) Add the cell membrane vesicle suspension obtained in step (1) dropwise on the surface of the super-hydrophilic coating obtained in step (2), incubate at 37°C for 2 hours, wash with deionized water for 3 tim...

Embodiment 2

[0038] A kind of cardiovascular implant intervention material / apparatus bio-film coating, its preparation method comprises the following steps:

[0039] (1) Centrifuge whole blood to obtain platelets, then resuspend them in PBS solution containing 1mM EDTA, then add protease inhibitors and freeze-thaw 5 times repeatedly, then wash, resuspend and sonicate in sequence to obtain platelet membrane vesicle suspension ; The protein concentration of the cell membrane vesicle suspension is 2mg / mL;

[0040] (2) Place the 316L stainless steel material in a sodium acetate solution containing 2mg / mL dopamine hydrochloride and 2mg / mL sodium periodate, react at a temperature of 30°C for 25min, wash with deionized water, and repeat this 3 times to obtain superhydrophilic coating;

[0041] (3) Add the cell membrane vesicle suspension obtained in step (1) dropwise on the surface of the super-hydrophilic coating obtained in step (2), incubate at 37°C for 2 hours, wash with deionized water for ...

Embodiment 3

[0043] A kind of cardiovascular implant intervention material / apparatus bio-film coating, its preparation method comprises the following steps:

[0044] (1) Centrifuge whole blood to obtain platelets, then resuspend them in PBS solution containing 1mM EDTA, then add protease inhibitors and freeze-thaw 5 times repeatedly, then wash, resuspend and sonicate in sequence to obtain platelet membrane vesicle suspension ; The protein concentration of the cell membrane vesicle suspension is 2mg / mL;

[0045] (2) Place the decellularized porcine pericardial material in a sodium acetate solution containing 2mg / mL dopamine hydrochloride and 2mg / mL sodium periodate, react at 30°C for 25min, wash with deionized water, repeat the process 3 times , to obtain a superhydrophilic coating;

[0046] (3) Add the cell membrane vesicle suspension obtained in step (1) dropwise on the surface of the super-hydrophilic coating obtained in step (2), incubate at 37°C for 2 hours, wash with deionized water ...

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Abstract

The invention discloses a cardiovascular implant intervention material / instrument biological film coating and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a cell membrane vesicle suspension; (2) constructing a super-hydrophilic coating on the surface of the substrate material; and (3) co-incubating the cell membrane vesicle suspension obtained in the step (1) and the super-hydrophilic coating obtained in the step (2), and cleaning with deionized water to obtain the cardiovascular implant intervention material / instrument biological membrane coating. The invention also comprises the cardiovascular implant intervention material / instrument biological film coating prepared by the method. According to the coating, the surface of a large-scale medical device is coated with a cell membrane for the first time, the anticoagulation performance and the anti-inflammatory performance of a blood contact material can be effectively enhanced, and the coating has the capacity of promoting endothelial repair and can be suitable for modification of medical interventional devices such as heart occluders, heart valves, intravascular stents and artificial blood vessels; the inflammatory reaction degree and the thrombosis risk after the instrument is implanted can be obviously reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a cardiovascular implant intervention material / device bio-film coating and a preparation method thereof. Background technique [0002] At present, biomimetic cell membrane is one of the research hotspots in the surface modification of biomaterials, and the coating of cell membrane on the surface of nanomaterials to optimize the properties of materials has been widely studied. As a natural component in the body, the cell membrane has good biocompatibility, and various membrane surface proteins endow it with multiple biological functions such as immune escape and promoting cell adhesion. Therefore, a lot of research has been done to coat the cell membrane on the surface of nanomaterials to improve the biological safety and biodistribution of materials, and it is widely used in the fields of anti-tumor therapy, atherosclerosis therapy, and thrombolytic thera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/28A61L27/54A61L31/08A61L31/14A61L31/16
CPCA61L27/507A61L27/28A61L27/50A61L27/54A61L31/08A61L31/14A61L31/16A61L2430/20A61L2430/22A61L2400/12A61L2300/42A61L2300/41A61L2300/606
Inventor 王云兵周仲毅罗日方杨立
Owner SICHUAN UNIV
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