Viscous biological sample liquefaction release combination product, kit, liquefaction release method and nucleic acid extraction, amplification and detection method
A combination product and biological sample technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of complex operation, insufficient, high cost, etc., and achieve good release effect and good compatibility
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Embodiment 1
[0262] Example 1. Liquefaction effect test
[0263] In this example, the liquefaction reagent of the experimental group was prepared by using guaifenesin, sodium hydroxide and zirconia beads. The final concentration was 100 mM guaifenesin, 10 mM sodium hydroxide, and 1 g / mL zirconia beads (with a diameter of 1 mm). The solvent used to prepare the reagent was purified water, which was recorded as the experimental group.
[0264] Four viscous sputum samples (I, II, III, IV) were collected, each of these sputum samples was thoroughly mixed separately, and six 2 mL samples were taken from each case, numbered 1-6. spare.
[0265] Add 6 mL of experimental group reagents to sample No. 1;
[0266] Add 6 mL of dithiothreitol solution ((2% (w / v) DTT) to sample No. 2;
[0267] Add 6 mL of sodium hydroxide solution (0.1 M) to sample No. 3;
[0268] To sample No. 4, add 6 mL of reagents prepared with a final concentration of 100 mM acetylcysteine, 10 mM sodium hydroxide, and 1 g / mL zir...
Embodiment 2
[0278] Example 2. Effect test of liquefaction components of different concentrations with zirconia beads
[0279] In this embodiment, the liquefaction reagent in the experimental group is composed of one or more of the following components: guaifenesin, sodium hydroxide, and zirconia beads.
[0280] Experimental group 1: The final concentration was 100 mM guaifenesin, 10 mM sodium hydroxide, 1 g / mL zirconia beads (with a diameter of 1 mm), and the solvent used to prepare the reagent was purified water.
[0281] Experimental group 2: The final concentration was 1 mM guaifenesin, 1000 mM sodium hydroxide, 1 g / mL zirconia beads (1 mm in diameter), and the solvent used for preparing the reagents was purified water.
[0282] Experimental group 3: The final concentration was 1 mM guaifenesin, 1 g / mL zirconia beads (with a diameter of 1 mm), and 0.1 mM sodium hydroxide. The solvent used to prepare the reagent was purified water.
[0283] Four viscous sputum samples (I, II, III, IV) ...
Embodiment 3
[0292] Example 3. Comparison of rapid detection of sputum samples after liquefaction with other methods
[0293] In this example, the liquefaction reagent of the experimental group was prepared by using guaifenesin, sodium hydroxide and zirconia beads. The final concentration was 100 mM guaifenesin, 10 mM sodium hydroxide, and 1 g / mL zirconia beads (with a diameter of 1 mm). The solvent used in the preparation of the reagent was purified water, which was used as the experimental group.
[0294] Four viscous sputum samples (I, II, III, IV) were collected, each of these sputum samples was thoroughly mixed separately, and five 2 mL samples were taken from each case, numbered 1-5. spare.
[0295] To sample No. 1, add 6 mL of the reagent of the experimental group; to sample No. 2, add 2.5 g of guanidine hydrochloride + 0.5 g of acetylcysteine + 2 g of polypropylene particles (the method disclosed in Patent Document No. CN108949748A); to sample No. 3 Add 6 mL of dithiothreitol s...
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