Composite indicator, quick test paper and method for detecting freshness of pork
An indicator and freshness technology, applied in the field of pork freshness detection, composite indicator, and quick test paper, can solve the problems of unfavorable market meat supervision and management, long testing time, high testing cost, etc., to improve market supervision. Effectiveness of management work
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Embodiment 1
[0023] (1) Judge the freshness of pork by sensory test:
[0024] Cut the fresh pork into pieces of about 10g, store them in a sealed bag, take a bag of pork every 2 hours, and test the properties of the pork’s touch, smell, color, and viscosity to judge the freshness of the pork over time.
[0025] The results are shown in Table 1. It can be found that the fresh pork began to change slightly after being placed at 15°C for 10-12 hours, the elasticity of the meat quality decreased, and it began to smell a little. The smell of ammonia, the meat starts to be wet and sticky, indicating that the freshness of the pork has begun to decline sharply.
[0026] Table 1 Pork - time sensory test
[0027]
[0028] Fresh pork changes over time, and metabolites such as ammonia are produced after protein deterioration, which will continuously increase the pH of the meat. According to Jingming's "Trial Discussion on the Method of Meat Freshness Laboratory Testing and Research" document, acc...
Embodiment 2
[0035] Choose the right pH indicator:
[0036] (1) Methyl red indicator to measure pH value:
[0037] Weigh 0.1g of methyl red, dissolve it in 100ml of absolute ethanol, and prepare a methyl red indicator; add 100ml of deionized water to a 150ml beaker, stir on a magnetic stirrer, insert a measured pH meter, and use 1% After dilute hydrochloric acid solution adjusts pH ≈ 4, drop 5 drops of methyl red indicator, and then slowly drop into 1% NaOH solution, observe and record the color jump pH value of methyl red indicator.
[0038] Such as figure 2 As shown, it can be seen that the methyl red indicator is yellow at pH=4.00-5.52, purple-red at pH=5.52-6.85, and purple at pH=7.02-9.21. pH = 6.6 is far away and cannot indicate the freshness of pork.
[0039] (2) Bromothymol blue indicator to measure pH value:
[0040]Weigh 0.1g of bromothymol blue, dissolve it in 100ml of absolute ethanol, and prepare bromothymol blue indicator; add 100ml of deionized water to a 150ml beaker, ...
Embodiment 3
[0055] Determination of pH value of pork by bromothymol blue sodium salt-bromocresol purple sodium salt composite indicator:
[0056] (1) Select 10g of pork that has been placed for 2h, 24h, and 34h, grind it with a meat grinder for 30s, then add 100ml of deionized water, pour it into a 150ml beaker, and make a pork infusion.
[0057] (2) After filtering the prepared pork infusions for 2h, 24h, and 34h, add 1 drop of bromothymol blue sodium salt-bromocresol purple sodium salt composite indicator respectively, observe the color, and judge the freshness of pork.
[0058] The result is as Figure 8 As shown, after adding the composite indicator to the pork extract that has been placed for 2 hours, the color is yellowish brown, which means that the pork is in a fresh state; after adding the composite indicator to the pork extract that has been placed for 24 hours, the color turns brown, that is, when the pork is in a sub-fresh state ; After adding the composite indicator to the p...
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