Sterile mouse preparation and breeding integrated isolation device

Abstract

The invention discloses a sterile mouse preparation and breeding integrated isolation device, which comprises a mobile operation table, and a hard transparent glass isolator, a soft transparent isolator and a stereoscopic microscope are placed on the top end surface of the mobile operation table, an opening of a first hard glass cylinder on the hard transparent glass isolator is connected with a connecting sleeve on the soft transparent isolator in a sealing mode, and the hard transparent glass isolator is arranged between an objective table and an objective lens of the stereoscopic microscope. A first glove sleeve and a second glove sleeve are arranged on the side walls, close to an operator, of the hard transparent glass isolator and the soft transparent isolator respectively, and a first operation glove and a second operation glove are arranged in the first glove sleeve and the second glove sleeve respectively. According to the sterile mouse preparation and breeding integrated isolation device, the sterile mouse preparation process and the sterile mouse breeding process are combined in a unified mode, the use cost is low, the structure is simple, the size is small, operation is convenient, and the large-scale production and preparation efficiency of the sterile mouse can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of preparation and breeding of sterile mice, and more specifically relates to an isolating device integrated with preparation and breeding of sterile mice. Background technique [0002] In recent years, due to the rapid development of microbiome, humans have increasingly realized that we all exist in the form of "super life organisms", that is, each individual is a symbiont of an organism and a large number of microorganisms. Microorganisms are mainly distributed in the tissue parts or passages where the body directly communicates or contacts with the external environment, such as the respiratory tract, digestive tract, genitourinary tract, and body surface, etc. Among them, intestinal microorganisms are various and huge in number, accounting for 10% of the total amount of microorganisms in the body. 78%, playing a very important role in pathological or physiological metabolic processes. [0003] In view of...

AI Technical Summary

Problems solved by technology

However, this preparation method has a large pollution risk, and the operation is complicated and the efficiency is not high; the second method is to use an isolator made of hard material (the top is light-transmitting glass) combined with the external use of a stereo microscope, so that Satisfies the technical requirements for the preparation of sterile mice by embryo transfer. However, this hard isolator has the disadvantages of bulkiness, corrosion resistance, high pollution risk, and high production cost, and is not suitable for large-scale production. In order to meet the working distance requirements of the stereo microscope, it is necessary to set a raised stage in the isolator, and place the stereo microscope on the top surface of the isolator, so that the distance between the stage and the stereo microscope can be satisfied. The working distance between the objective lenses is required. Although this method does not need to sterilize the stereo microscope, the isolator is equipped with a raised stage inside, so that the operator's arm needs to be operated all the time. Maintain the same height as the raised stage for operation. After a long time, the operator's arms will be sore and the user experience will be poor. The third method is to sterilize the stereomicroscope and pass it into the isolator for use. It is difficult for the microscope to meet the requirements of complete sterilization without damaging its parts

Therefore, it is not difficult to find that the current preparation method of sterile mice has the dilemma of low preparation efficiency and lack of special isolators, so it cannot fully meet the goal of large-scale production of sterile mice, which limits the wide application and development of sterile mice. further development

Images