Method for creating autoimmune hepatitis animal model

An autoimmune, animal model technology, applied in the field of creation of autoimmune hepatitis animal models, can solve the atypical characteristics of liver fibrosis, the inability to understand the pathogenesis and molecular mechanism of autoimmune hepatitis more broadly and accurately, and the inability to Issues in understanding and research of autoimmune hepatitis

Pending Publication Date: 2022-03-22
CHANGZHI MEDICAL COLLEGE
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AI Technical Summary

Problems solved by technology

[0004] 1) The animal model represented by concanavalin-induced immune liver injury only simulates the characteristics of inflammation and cannot fully understand and study autoimmune hepatitis;
[0005] 2) Animal models represented by adenoviral vectors that mimic antigens cannot produce autoimmune hepatitis in different mice, and the characteristics of liver fibrosis are not typical, so it is impossible to understand the pathogenesis and pathogenesis of autoimmune hepatitis more extensively and accurately. molecular mechanism

Method used

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  • Method for creating autoimmune hepatitis animal model

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Effect test

Embodiment 1

[0093] like figure 1 As shown, a method for creating an autoimmune hepatitis animal model comprises the following steps:

[0094] (1), d0 days, 48 ​​mice were divided into control group and experimental group, wherein:

[0095] The 24 mice in the control group were divided into four groups on average, namely the control group A, the control group B, the control group C and the control group D;

[0096] The 24 mice in the experimental group were divided into four groups on average, namely the test group A, the test group B, the test C group and the test D group;

[0097] The 24 mice in the test group were intraperitoneally injected with CCl 4 , CCl 4 The injection dose is 0.5ml / kg, and

[0098] On day -d2 two days before, 24 mice in the test group were all injected with plasmid CYP2D6 (plasmid CYP2D6 referred to as pCYP2D6) by tail vein at a concentration of 300 μg / time / mouse;

[0099] (2), five days later, on day d5, 24 mice in the test group were injected with plasmid CY...

Embodiment 2

[0135] like figure 1 As shown, a method for creating an autoimmune hepatitis animal model comprises the following steps:

[0136] On days S1 and d0, 48 mice were divided into control group and experimental group, in which:

[0137] The 24 mice in the control group were divided into four groups on average, namely the control group A, the control group B, the control group C and the control group D;

[0138] The 24 mice in the experimental group were divided into four groups on average, namely the test group A, the test group B, the test C group and the test D group;

[0139] The 24 mice in the test group were injected with adenovirus (Ad) through the tail vein, and the injection dose was 2×10 9 pfu, and

[0140] On day -d2 two days before, 24 mice in the test group were all injected with plasmid CYP2D6 (plasmid CYP2D6 referred to as pCYP2D6) by tail vein at a concentration of 300 μg / time / mouse;

[0141] S2. On day d5 after five days, all 24 mice in the test group were injec...

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Abstract

The invention discloses a method for creating an autoimmune hepatitis animal model, which comprises the following steps: (1) injecting CCl4 into the abdominal cavity of a mouse on the d0 day once every 7 days, and stopping injecting CCl4 on the fourteenth day; (2) intravenously injecting plasmids CYP2D6 into the tail of the mouse for the first time 2 days before CCl4 injection, then injecting the plasmids CYP2D6 once every 7 days, and stopping injecting the plasmids CYP2D6 until the d54 day; and (3) respectively detecting liver inflammation, liver injury, autoantibody production and cytokine expression of the mouse on d30 days, d40 days, d50 days and d60 days, comparing the results of the liver inflammation, the liver injury, the autoantibody production and the cytokine expression with those of a normal mouse, taking the results as contrasts at different time points, comparing, and as time goes on, determining that the results of the liver inflammation, the liver injury, the autoantibody production and the cytokine expression are different. And if the detected indexes become more and more obvious, the modeling is successful.

Description

technical field [0001] The invention relates to a method for creating a model, in particular to a method for creating an animal model of autoimmune hepatitis. Background technique [0002] Autoimmune hepatitis (AIH) is a chronic immune-mediated autoimmune liver disease characterized by hypergammaglobulinemia, circulating autoantibodies, and interface hepatitis. If long-term liver inflammation and damage persist, it can lead to liver fibrosis, cirrhosis, and liver cancer. Although the pathogenesis of AIH may be related to genetic susceptibility, molecular mimicry, and impaired immune regulation, to find specific molecular targets and pathogenesis requires an animal model that can well simulate clinical features. Explore the molecular mechanisms and pathogenesis of the disease. [0003] At present, animal models of autoimmune hepatitis either only simulate the characteristics of inflammation, but cannot simulate the characteristics of autoantibodies and liver fibrosis, or on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02G01N33/573G01N33/543G01N33/68
CPCA01K67/02G01N33/573G01N33/543G01N33/6863G01N33/6869G01N33/6866
Inventor 池刚冯作化裴晋红和源
Owner CHANGZHI MEDICAL COLLEGE
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