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Cream for treating cow mastitis and preparation method thereof

A technology for mastitis and dairy cows, applied in the field of cream for the treatment of dairy cows mastitis and its preparation, can solve the problems of severe drug resistance of Staphylococcus aureus derived from dairy cows, and achieve good antibacterial effect

Pending Publication Date: 2022-03-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Staphylococcus aureus is the main pathogen causing mastitis in dairy cows. At present, the treatment of mastitis in dairy cows mainly relies on antibiotics, but the long-term use of antibiotics has made the drug resistance of Staphylococcus aureus from dairy cows more and more serious

Method used

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  • Cream for treating cow mastitis and preparation method thereof
  • Cream for treating cow mastitis and preparation method thereof
  • Cream for treating cow mastitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment is the bacteriostasis experiment of raspberry leaf extract.

[0025] Dried the raspberry leaves, crushed them into powder, weighed 30g of leaves and 2g of tartaric acid, added 750mL of ethanol solution with a volume fraction of 60%, and assisted extraction with 200w ultrasonic power for 30min at 60-65°C. After the extraction was completed, the insoluble matter was filtered off, and the filtrate was concentrated by rotary evaporation until it was concentrated to 20 mL, and extracted with an equal volume of ethyl acetate for decolorization, and the extracted aqueous phase was then extracted with an equal volume of n-butanol. Finally, the n-butanol phase was rotary evaporated to obtain solid matter, and raspberry leaf extract was obtained.

[0026] Measure the OD of Staphylococcus aureus cultured for 12 hours by double dilution method 600 Post-determined OD 600 1, and then diluted 1:100 in 10 EP tubes respectively, adding 20mg / mL raspberry leaf extract or...

Embodiment 2

[0033] Dry the raspberry leaves, break them into powder, weigh 30g of leaves and 2g of tartaric acid, add 750mL of ethanol solution with a volume fraction of 75%, and use 150w power ultrasonic to assist extraction for 60min at 60-65°C. After the extraction was completed, the insoluble matter was filtered off, and the filtrate was concentrated by rotary evaporation until it was concentrated to 20 mL, and extracted with an equal volume of ethyl acetate for decolorization, and the extracted aqueous phase was then extracted with an equal volume of n-butanol. Finally, the n-butanol phase was rotary evaporated to obtain solid matter, and raspberry leaf extract was obtained.

[0034]The growth curve of Staphylococcus aureus was determined using the extracted raspberry leaf extract, and the staphylococcus aureus derived from dairy cows was subcultured three times, and the OD 600 The initial value of 0.05 was transferred to 50mL TSB, and the OD was measured every 2 hours 600 The value...

Embodiment 3

[0039] This embodiment provides a cream for treating mastitis in dairy cows, the main components of which include raspberry leaf extract, tartaric acid, disodium edetate, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben Esters, Glyceryl Monostearate, White Petrolatum, Cetyl Alcohol. Wherein, the aqueous phase includes raspberry leaf extract, tartaric acid, edetate disodium, and vitamin C, wherein raspberry leaf extract accounts for 5%, tartaric acid accounts for 1%, and edetate disodium accounts for 0.2% , vitamin C accounted for 1.5%. The oil phase includes glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white petrolatum, and cetyl alcohol, wherein glycerin accounts for 25%, white petrolatum accounts for 30%, liquid Paraffin wax accounts for 30%, salicylic acid accounts for 6%, vitamin E accounts for 3%, ethylparaben accounts for 3%, glyceryl monostearate accounts for 2%, and cetyl alcohol accounts for 1%.

[0040] The specific preparat...

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Abstract

The invention provides emulsifiable paste for treating mastitis of dairy cows, and belongs to the technical field of treatment of mastitis of dairy cows. The cream for treating dairy cow mastitis contains a raspberry leaf extract, and a preparation method of the raspberry leaf extract comprises the following steps: drying raspberry leaves, crushing, mixing the crushed raspberry leaves with tartaric acid, adding an ethanol solution for extraction, filtering out insoluble substances after extraction, concentrating, extracting and decolorizing with ethyl acetate, and drying to obtain the raspberry leaf extract. And extracting the extracted water phase by using n-butyl alcohol. And finally, carrying out rotary evaporation on the n-butyl alcohol phase to obtain a solid substance, namely the raspberry leaf extract. The emulsifiable paste can quickly cure dairy cow mastitis which cannot be cured by antibiotics and inhibit multi-drug-resistant staphylococcus aureus for a long time, drug resistance is not found after long-time tracking, and the emulsifiable paste is remarkably better than existing antibiotic products on dairy cows with repeated mastitis.

Description

technical field [0001] The invention relates to the technical field of treating cow mastitis, in particular to a cream for treating cow mastitis and a preparation method thereof. Background technique [0002] Mastitis is the most common and costly disease of dairy cows. It affects the health of cows and causes economic losses through reduced milk production, reduced milk quality, premature culling, veterinary treatment and antibiotic treatment. According to statistics, about one-third of the world's dairy cows suffer from various types of mastitis, and the global loss caused by mastitis reaches tens of billions of dollars every year. Staphylococcus aureus is the main pathogen causing mastitis in dairy cows. At present, the treatment of mastitis in dairy cows mainly relies on antibiotics, but the long-term use of antibiotics has made the drug resistance of Staphylococcus aureus from dairy cows more and more serious. [0003] Raspberry is an ancient traditional Chinese medic...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K9/06A61K47/22A61P15/14A61P31/04A61P31/02A61K31/194
CPCA61K36/73A61K31/194A61K9/06A61K9/0041A61K47/22A61P15/14A61P31/04A61P31/02A61K2236/333A61K2236/51A61K2300/00
Inventor 王云生薛挺张妞妞汪晖钱薇周祺芳周欣月钱一萱汪岳
Owner ANHUI AGRICULTURAL UNIVERSITY
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