SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum character and application
A molecular marker and black technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of affecting carcass appearance, affecting economic effects, and less black peritoneum traits of chickens, so as to improve Newcastle disease Antibody response level, effect of reducing number
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Embodiment 1
[0030] 1 Test animals:
[0031] The experimental group used was F, Institute of Animal Science, Guangdong Academy of Agricultural Sciences. 2 The first-generation yellow-feather broiler hybrid resource population, the parents are yellow-feather broiler fast large male line A and slow Huiyang bearded chicken H line. Among them, A line mainly selects growth traits as the main goal and has undergone more than 10 generations of closed population selection. Yu, the H series is a Guangdong local variety, native to Huiyang, Guangdong Province. The yellow feather broiler resource group F 2 The generation is divided into 6 batches for hatching and hatching.
Embodiment 1
[0032] Embodiment has used altogether 395 F with phenotype 2 Generation individuals, including 212 roosters and 183 hens, came from 8 half-sib families. At the age of 25 and 50 days, the commercial Newcastle disease virus LaSota strain live vaccine was immunized twice respectively. Slaughter at the age of 91 days, such as figure 1 According to the previous research, according to the proportion of the melanin deposition area in the peritoneum and the color of the melanin deposition area, the characteristics of the melanin peritoneum were graded, the serum was separated, and the NDV antibody response was detected by the indirect ELISA method. The specific operation is as follows:
[0033] At the age of 25 days and 50 days respectively, they were immunized with commercial Newcastle disease virus LaSota strain (Interve International B.V., Boxmeer, Netherlands) live vaccine according to the standard dose given in the vaccine instructions. Serum samples were collected from the chi...
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