SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum character and application

A molecular marker and black technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of affecting carcass appearance, affecting economic effects, and less black peritoneum traits of chickens, so as to improve Newcastle disease Antibody response level, effect of reducing number

Active Publication Date: 2022-03-25
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

It seriously affects the appearance of the carcass and affects the economic effect
And the trait of black belly is relatively common in yellow-feathered broiler chickens. At the age of 3 weeks, 5%-95% of Huiyang bearded chickens have black peritoneum, but there are still relatively few studies on the trait of black peritoneum in chickens.

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum character and application
  • SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum character and application
  • SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum character and application

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Experimental program
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Effect test

Embodiment 1

[0030] 1 Test animals:

[0031] The experimental group used was F, Institute of Animal Science, Guangdong Academy of Agricultural Sciences. 2 The first-generation yellow-feather broiler hybrid resource population, the parents are yellow-feather broiler fast large male line A and slow Huiyang bearded chicken H line. Among them, A line mainly selects growth traits as the main goal and has undergone more than 10 generations of closed population selection. Yu, the H series is a Guangdong local variety, native to Huiyang, Guangdong Province. The yellow feather broiler resource group F 2 The generation is divided into 6 batches for hatching and hatching.

Embodiment 1

[0032] Embodiment has used altogether 395 F with phenotype 2 Generation individuals, including 212 roosters and 183 hens, came from 8 half-sib families. At the age of 25 and 50 days, the commercial Newcastle disease virus LaSota strain live vaccine was immunized twice respectively. Slaughter at the age of 91 days, such as figure 1 According to the previous research, according to the proportion of the melanin deposition area in the peritoneum and the color of the melanin deposition area, the characteristics of the melanin peritoneum were graded, the serum was separated, and the NDV antibody response was detected by the indirect ELISA method. The specific operation is as follows:

[0033] At the age of 25 days and 50 days respectively, they were immunized with commercial Newcastle disease virus LaSota strain (Interve International B.V., Boxmeer, Netherlands) live vaccine according to the standard dose given in the vaccine instructions. Serum samples were collected from the chi...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to chicken black peritoneum traits and application. Relates to the technical field of chicken marker-assisted selection. According to the invention, black peritoneal characters are graded, NDV antibody response is detected, and genetic typing is carried out. And carrying out GWAS analysis by taking the incubation batch, the gender and the first three main components as fixed effects. According to the invention, the allele A is used as an SNP (Single Nucleotide Polymorphism) site (rs317439499) to obtain a remarkable SNP site (rs317439499), and then correlation analysis is carried out to obtain that the allele A has a very remarkable negative effect on black peritoneal characters and has a remarkable positive effect on Newcastle disease antibody response level; the allele G has an extremely remarkable positive effect on the black peritoneal character and has a remarkable negative effect on the Newcastle disease antibody response level. The molecular marker SNP site can be used for molecular marker-assisted breeding of chicken black peritoneum traits. The genetic marker is used for breeding, so that the number of black peritoneal individuals of a group can be reduced, and the Newcastle disease antibody response level can be improved.

Description

technical field [0001] The invention relates to the technical field of chicken marker-assisted selection, in particular to a SNP molecular marker related to chicken black peritoneum traits and its application. Background technique [0002] Chicken is the second most consumed meat in my country, and the per capita consumption of chicken in my country has continued to grow in recent years. In order to prevent the spread of diseases and ensure the quality and safety of live poultry products, the high-quality broiler industry, which mainly trades live poultry, will gradually transform to chilled and slaughtered. Therefore, the selection and breeding of broiler carcass appearance traits that directly affect the economic benefits of the breed, such as black peritoneum traits, have been paid attention to. [0003] The chicken black peritoneum trait is characterized by a dark blue appearance of the abdominal skin, and when the skin is removed, black connective tissue appears. It s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G16B20/20
CPCC12Q1/6888G16B20/20C12Q2600/124C12Q2600/156
Inventor 周广源罗成龙刘天飞瞿浩舒鼎铭王艳
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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