An easy-to-use porous brain slice incubation device

An incubation device and brain slice technology, which is applied in the field of medical devices to achieve the effects of improving the survival rate, improving the survival rate and activity, and stabilizing the water level.

Active Publication Date: 2022-05-13
ACADEMY OF MILITARY MEDICAL SCI
View PDF54 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above problems, the present invention provides a convenient-to-use porous brain slice incubation device, which effectively ensures a suitable incubation environment, and at the same time solves the problem that multiple incubations can be incubated together, and single incubation can effectively save the incubation solution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An easy-to-use porous brain slice incubation device
  • An easy-to-use porous brain slice incubation device
  • An easy-to-use porous brain slice incubation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Please refer to Figure 1-Figure 3 , a kind of convenient-to-use porous brain slice incubation device in the present embodiment, it comprises incubation device body 1; Incubation chamber 11 and oxygen channel 12 are arranged in the incubation device body 1, and oxygen channel 12 communicates with incubation room 11, and oxygen channel 12 is connected to an oxygen supply pipeline 13; an incubation plate 14 is arranged inside the incubation chamber 11, and the incubation plate 14 is movably arranged inside the incubation chamber 11; the incubation plate 14 is provided with at least one incubation hole 15, and the bottom of the incubation hole 15 is provided for carrying brain slices / Incubation net 16 of the tissue sheet; a spacer is set in the incubation chamber 11, and the spacer divides the interior of the incubation chamber 11 into different spaces; a temperature control device 2 is set outside the incubation device body 1, and the temperature control device 2 is still...

Embodiment 2

[0048] Please refer to Figure 1-Figure 6 , on the basis of Example 1, the present embodiment carries out the following specific settings to the incubation device body 1: a cover plate 17 is set on the upper end of the incubation device body 1, and the cover plate 17 covers the incubation device body 1 to ensure that the incubation chamber 11 and the oxygen passage 12 of tightness. The shape of the incubation device body 1 is not limited. In this embodiment, the shape of the incubation device body 1 is set as a cube, but the shape of the incubation device body 1 can also be set as a cylinder or a shape that can be implemented smoothly. The shape of the incubation hole 15 is also not limited. The incubation hole 15 in this embodiment is square, and may also be circular or other convenient shapes. The shapes of the incubation chamber 11 and the incubation plate 14 match. In the present embodiment, the shapes of the incubation chamber 11 and the incubation plate 14 are square, a...

Embodiment 3

[0053] Please refer to Figure 7-Figure 9 , On the basis of Embodiment 2, this embodiment specifically sets the spacer: the spacer in this embodiment divides the interior of the incubation chamber 11 into different spaces. The spacer comprises a partition plate one 19 located in the incubation chamber 11, a partition plate two 110 positioned at the oxygen passage 12, and a support plate 111 arranged on the inner wall of the oxygen passage 12; the partition plate one 19 and the partition plate two 110 are perpendicular to each other , partition one 19 partitions the incubation chamber 11, partition two 110 partitions the oxygen passage 12, and the incubation solution is placed in the chamber for placing brain slices / tissue slices; Through holes 112; during use, the second partition plate 110 is placed on the top of the annular plate, the first partition plate 19 is located in the incubation chamber 11, and then the through hole 112 is aligned with the first partition plate 19, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a convenient-to-use porous brain slice incubation device, which comprises an incubation device body; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage communicates with the incubation chamber, and the oxygen passage connects an oxygen supply pipeline; the interior of the incubation chamber The incubation board is set, and the activity of the incubation board is set inside the incubation chamber; the incubation board is provided with at least one incubation hole, and an incubation net for carrying brain slices / tissue slices is arranged under the incubation hole; the incubation room is set to divide the incubation room into different spaces. Components; a temperature control device is installed outside the incubation device body, and the temperature control device still uses the principle of the water bath method; because incubation under water bath conditions can maintain the temperature well, the temperature is controlled and heated evenly; in this way, the brain slices / tissues The slice is placed in the incubation hole, and the oxygen supply pipeline and oxygen channel are used to provide oxygen to the brain slice / tissue slice and incubate; setting up the spacer can effectively avoid wasting the incubation solution when incubating a single brain slice.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a convenient-to-use porous brain slice incubation device. Background technique [0002] Brain slice is a commonly used specimen in current neuroscience research. The method is to remove the brain tissue or spinal cord from the animal body and cut it into 200-500μm thin slices in artificial cerebrospinal fluid. These flakes retain the activity and function of part of the neural tissue, compared with discrete or cultured cells, they have the advantage of retaining a relatively complete neural circuit and extracellular environment; compared with in vivo, the influencing factors are relatively simple and more predictable When preparing brain slices, multiple brain slices are usually cut out for the brain region of interest, and then the brain slices are placed in artificial cerebrospinal fluid for incubation. The common method is to place all the brain slices in a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/44
CPCG01N1/44
Inventor 吴海涛邵靖媛赵哲刘凤姣吴燕常欣李燊
Owner ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap