Novel aureobasidium pullulans strain and use thereof

A technology of Aureobasidium pullulans and strains, which is applied in the direction of fungi, topical antibacterial agents, and cosmetic preparations, etc., which can solve the problems of insufficient efficiency and economy, complex production processes, etc., and achieve excellent wound healing and skin inhibition. Harmful bacteria, excellent effect

Pending Publication Date: 2022-04-01
LG HOUSEHOLD & HEALTH CARE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, said strain produces melanin, so the culture broth of the strain passes through yellow and finally becomes black, thus, when producing a product containing the extract, ferment or pullulan of the strain, it is necessary to include a purification / decolorization process, thereby There are disadvantages that the product production process becomes complicated and the efficiency and economy are insufficient

Method used

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  • Novel aureobasidium pullulans strain and use thereof
  • Novel aureobasidium pullulans strain and use thereof
  • Novel aureobasidium pullulans strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: Discovery of the novel strain isolated from dendrobium (Dendrobium)

[0075] The present inventors have isolated a novel pullulan-producing pullulan in order to discover a microorganism that does not produce black pigment among Aureobasidium pullulans microorganisms known as pullulan-producing microorganisms. Aureobasidium pullulans (Aureobasidium pullulans) strain, which is a bacterial strain isolated from plants of the genus Dendrobium (Dendrobium), which are included in the National Aeronautics and Space Administration (NASA (National Aeronautics and Space Administration)) In designated air-purifying plants.

[0076] As a result of NCBI blast analysis of the following ITS sequence of the isolated strain, the identity with the existing strain is 98.54 to 99.26%, so it is confirmed that the isolated strain is different from the existing strain strains.

[0077] ITS sequence (sequence number 1) of OFY11-1 bacterial strain:

[0078] TACTGGCAACCTACTGATC...

Embodiment 2

[0081] Embodiment 2: the manufacture of novel strain fermentation product

[0082] In this example, in order to produce the fermented product of the novel strain, the novel strain OFY11-1 was subjected to pre-culture and main culture for 5 to 7 days. For pre-cultivation, OFY11-1 bacteria were inoculated in a container containing a commercial medium PDB (Potato Dextrose Broth), and shaking culture was performed at 28° C. at 160 rpm. Then, the preculture solution was inoculated into the container containing the main medium, and shaking culture was performed at 28° C. at 160 rpm.

[0083]

[0084]40g / L of sucrose (sucrose), 1g / L of yeast extract, 0.3g / L of (NH 4 ) 2 SO 4 , 1g / L NaCl, 5g / L K 2 HPO 4 , 0.2g / L MgSO 4

[0085] Then, as a result of centrifuging the main culture solution and collecting the supernatant, the culture solution of the OFY11-1 bacterium was obtained. Since the culture solution is produced as a result of decomposing organic compounds by OFY11-1, i...

Embodiment 3

[0086] Example 3: Confirmation that the fermented product of the new strain does not produce black pigment

[0087] In order to confirm that the new bacterial strain isolated from the plant of the genus Dendrobium does not produce black pigment, the fermented product of the announced bacterial strain is produced in the same manner as in Example 2, and the fermented product of the new bacterial strain produced in Example 2 and the announced bacterial strain are fermented. Compare the colors of objects.

[0088] - Announced strain: Aureobasidium pullulans KCTC 6459

[0089] - Novel strain: Aureobasidium pullulans OFY11-1

[0090] As a result, it was confirmed that the fermented product of the announced strain was black, and the fermented product from which the cells were removed was dark yellow, and when it was applied to products such as cosmetics or quasi-drugs, an additional purification / decolorization process was required. On the contrary, it was confirmed that the ferme...

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Abstract

The invention relates to an aureobasidium pullulans OFY11-1 strain, a preparation method of the aureobasidium pullulans OFY11-1 strain and a preparation method thereof. A cosmetic composition comprising the strain or a fermentation product thereof, and a method for producing the same; a pharmaceutical composition comprising the strain or a fermentation product thereof, and a method for producing the same; a cosmetic composition or a pharmaceutical composition comprising Pulullan isolated from the strain or a fermentation product thereof; and a method for producing pullulan which is isolated from the strain or a fermented product thereof. The strain of the present invention has an excellent efficacy of not producing a black pigment, and thus does not require an additional decolorization/purification process when producing the composition and pullulan of the present invention, and thus can economically and efficiently produce the composition or pullulan.

Description

technical field [0001] The present invention relates to a strain of Aureobasidium pullulans OFY11-1; a cosmetic material composition comprising the strain or its fermented product and a production method thereof; a quasi-drug composition comprising the strain or its fermented product; and Production method thereof; cosmetic composition or quasi-drug composition comprising pullulan isolated from said strain or its fermented product; and pullulan isolated from said bacterial strain or its fermented product manufacturing method. Background technique [0002] Pullulan is an extracellular secreted polysaccharide produced by fungi, generally a substance produced by a strain of Aureobasidium pullulans as a black yeast, the sugar produced by fermenting said strain is isolated It is then obtained through a purification process. It is well known that pullulan is easily soluble in water and has low viscosity but strong adhesion, and is also used as a cosmetic additive for improving t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/10A61K8/9728A61K8/73A61Q19/00A61Q19/02A61Q17/00A61K36/06A61K31/715A61K36/8984A61P17/00A61P17/02A61P31/02A61P29/00C12R1/645
CPCC12N1/14C12P19/10A61K8/99A61K8/73A61Q19/02C12R2001/645
Inventor 尹圣熏金夏永丁海愍黄承镇
Owner LG HOUSEHOLD & HEALTH CARE LTD
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