Formaldehyde-free fixing liquid capable of being used for fixing various fresh tissues and fixing method

Abstract

The invention discloses a formaldehyde-free fixing liquid for fixing various fresh tissues, which comprises a liquid A and a liquid B, the liquid A and the liquid B are used for sequentially carrying out first fixation and second fixation on the fresh tissues, various substances in the various tissues can be effectively fixed, and the fixed tissues are not obvious in expansion and contraction, so that the tissues keep the morphological structure in a living state as far as possible; and subsequent slicing and pathological examination are facilitated.

Description

technical field [0001] The invention belongs to the technical field of medical histopathology, and in particular relates to a formaldehyde-free fixative solution and a fixation method that can be used for fixing various fresh tissues. Background technique [0002] In histopathology, it is necessary to properly and effectively fix the collected fresh tissues in time to prevent tissue autolysis and corruption, to precipitate or coagulate intracellular proteins, fats, sugars, enzymes and other components, and to make tissues and cells Keep the morphological structure in the living state as much as possible, so that the subsequent work such as slicing can be carried out smoothly. [0003] Fixative is a commonly used tissue fixative. The traditional tissue fixative is formalin (formaldehyde aqueous solution), which has the problems of volatile formaldehyde and high toxicity. It is easy to pollute the environment and cause damage to the operator's body during use. . At the same ...

AI Technical Summary

Problems solved by technology

However, if one kind of fixative is used for each type of human tissue, it is necessary to prepare a large number of fixatives and mark each fixative separately, which is a huge workload.

[0004] As a kind of tissue fixative, ethanol has both fixation and dehydration effects on tissue cells. When using ethanol as a fixative, if high-concentration ethanol is directly used, the surface of the biological material will be dehydrated rapidly to form a layer similar to a protective film. structure, which affects the fixation and dehydration inside the tissue, so that the concentration of ethanol is required to have a gradient (required concentration from high to low), and the concentration gradient generally used in the prior art is 50%-70%-80%-95% - Absolute ethanol, the span of each gradient of ethanol concentration varies greatly, and the fixed dehydration effect is not good. If you want to use ethanol solutions with small spans between gradients, you need to prepare many groups of ethanol solutions with different concentrations, and frequent fixes are required. Liquid replacement, preparation, fixation, and dehydration are all cumbersome to operate, and the fixation method in the prior art is an open processing method. When the fixative needs to be replaced many times, it is easy to cause sample contamination.

Images