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Surface enhanced Raman scattering substrate and application method thereof

A technology of surface-enhanced Raman and application methods, applied in Raman scattering, measurement devices, instruments, etc., can solve the problems of Raman signal enhancement and blocking of substances that cannot be measured, and achieve the effect of avoiding residues and reducing use costs.

Pending Publication Date: 2022-04-12
陕西未来健康科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently using SERS substrates to test cells, the preparation of cell samples in the liquid phase is a relatively big problem. The difficulty lies in the fact that cell culture fluid or body fluids remain unavoidably while transferring cells to the surface of the SERS substrate; After the crystallization of salts and proteins, the cells and the Raman hotspots on the SERS substrate will be blocked; the vertical range of the Raman hotspots on the SERS substrate is often only tens of nanometers, and it cannot be effectively tested after being blocked. Substance undergoes Raman signal enhancement, which is unacceptable
For the above problems, there is still a lack of effective solutions and SERS substrates, and there is an urgent need for a new SERS substrate that can effectively avoid the influence of salts and protein crystallization

Method used

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  • Surface enhanced Raman scattering substrate and application method thereof
  • Surface enhanced Raman scattering substrate and application method thereof
  • Surface enhanced Raman scattering substrate and application method thereof

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Effect test

Embodiment 1

[0050] In the embodiment of the present invention, the cells in the clinical cerebrospinal fluid are collected by using a surface-enhanced Raman scattering substrate combined with a centrifugal sedimentation method. Wherein, the nanostructure of the surface-enhanced Raman scattering chip is an Au nano-pyramid, which is fixed on a slide glass by means of epoxy resin bonding.

[0051] The centrifugation parameter used was 700r / min, and the centrifugation time was 5min. After centrifugation, the cells in the cerebrospinal fluid are enriched and collected on the surface-enhanced Raman scattering chip, and there is almost no influence of crystallization of salts and proteins, such as Figure 4 shown. The cells on the surface-enhanced Raman scattering chip are easy to perform Raman testing, and the surface-enhanced Raman scattering chip shows excellent enhancement ability.

Embodiment 2

[0053] In the embodiment of the present invention, a surface-enhanced Raman scattering substrate combined with a centrifugal sedimentation method is used to collect cells in clinical pleural fluid. Among them, the nanostructure of the surface-enhanced Raman scattering chip is an Ag nano-bow tie, which is fixed on the glass slide by a mechanical fitting method.

[0054] The centrifugation parameter used is 900r / min, and the centrifugation time is 1min. After centrifugation, the cells in the ascites were enriched and collected on the surface-enhanced Raman scattering chip, and there was almost no influence of crystallization of salts and proteins.

Embodiment 3

[0056] In the embodiment of the present invention, the cells in the urine of clinical patients are collected by using a surface-enhanced Raman scattering substrate combined with a centrifugal sedimentation method. Wherein, the nanostructure of the surface-enhanced Raman scattering chip is Cu nanoholes, which are fixed on the glass slide by bonding.

[0057] The centrifugation parameter used was 800r / min, and the centrifugation time was 3min. After centrifugation, the cells in urine were enriched and collected on the surface-enhanced Raman scattering chip, and there was little effect of crystallization of salts and proteins.

[0058] To sum up, the embodiment of the present invention discloses a surface-enhanced Raman scattering substrate for collecting cells in the liquid phase with a centrifugal smear, and the surface-enhanced Raman scattering substrate is compatible with a cell slide centrifugal sedimentation The cells in phase were centrifuged onto a surface-enhanced Raman...

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Abstract

The invention discloses a surface enhanced Raman scattering substrate and an application method thereof, the surface enhanced Raman scattering substrate comprises a carrier and a surface enhanced Raman scattering chip; the surface enhanced Raman scattering chip is fixedly arranged at a preset position of the carrier; the cell collector is used for collecting cells in a liquid phase based on a centrifugal smear method; wherein in the centrifugal smear method, the used centrifugal parameter is 700 to 900 r / min, and the centrifugal time is 1 to 5 minutes. The surface enhanced Raman scattering substrate provided by the invention can effectively collect cells in a liquid phase environment, and can greatly avoid the residue of cell culture fluid or body fluid.

Description

technical field [0001] The invention belongs to the technical field of assay devices, in particular to a surface-enhanced Raman scattering substrate and an application method thereof. Background technique [0002] Surface-enhanced Raman scattering (SERS) is a spectroscopic technique that combines modern laser spectroscopy with the excitation optical properties of metallic nanostructures. The Raman spectrum of the molecule. SERS has broad application prospects in the fields of biophysics and biomedicine spectroscopy. It can provide ultra-sensitive detection and characterization of biophysics and biomedicine-related molecules and processes, and has extremely high spatial vibrational spectral resolution. At present, SERS has been widely used in the medical field, and there are a lot of researches on biological particles of different scales, including nucleic acids, proteins, viruses, bacteria and cells; at the same time, based on the rapid and sensitive detection method of SER...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/01
Inventor 赵钢牛刚任巍武和平姜陆月谢瑱
Owner 陕西未来健康科技有限公司
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