The invention discloses a
mass spectrum Lamannite combined substrate and a preparation method, relates to the technical field of analysis and detection, and provides a difunctional substrate which can support
electrospray ionization mass spectrometry detection and surface enhanced Raman detection at the same time, does not interfere with each other and is suitable for on-site rapid operation. The substrate comprises a substrate film and at least one directional liquid
transmission channel positioned on the surface of the substrate film, and a Raman detection area and a
mass spectrum detection area are arranged in the channel; the
mass spectrometry detection area is arranged at the
tail end of the channel, the
tail end is of a pointed structure, and a substrate film at the
tail end comprises a
metal conductive layer; the Raman detection area is arranged at any position, which is not overlapped with the
mass spectrum detection area, in the channel, and a
metal nanostructure is arranged on a substrate film of the Raman detection area and is used for realizing surface-enhanced Raman detection; after the liquid sample flows through the Raman detection area, the Raman detection device performs Raman detection on the liquid sample, and when the liquid sample flows to the
mass spectrum detection area, the
mass spectrum detection device performs mass spectrum detection on the liquid sample.