The invention belongs to the technical field of biosensors and specifically relates to a fluorescence enhanced type aptamer sensor as well as a preparation method and application thereof. A metal nanostructure is guided into a fluorescent molecular detection system, aptamer for modifying a certain amount of basic groups A is combined with metal nanoparticles through sulfydryl, and plasma resonancegenerated by the metal nanoparticles is utilized to improve fluorophore excitation efficiency; thus, fluorescent radiation amplitude is remarkably improved, and the purpose of amplifying fluorescencesignals is achieved. Meanwhile, the fluorescence enhanced type aptamer sensor which is used for detecting low-concentration target objects is provided by being combined with specificity selection ofaptamer, so that flexibility, accuracy and specificity of analysis detection are ensured, and detection of polyphyly of the target object is also achieved. Meanwhile, the fluorescence enhanced type aptamer sensor has the characteristics of low cost, no pollution and wider detection range.