A kit and detection method for expanding the quantitative detection range

A technology of quantitative detection and detection method, applied in the field of in vitro diagnosis and immunoassay, can solve the problem of narrow quantitative detection range of macromolecular protein, and achieve the effect of delaying the HOOK effect, overcoming the influence and simple operation.
CN114324901BActive Publication Date: 2022-06-07天津中新科炬生物制药股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
天津中新科炬生物制药股份有限公司
Publication Date
2022-06-07

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Abstract

The present invention proposes a kit and a detection method for expanding the range of quantitative detection, including a carrier, the carrier is provided with a marker site, a T line, and a C line, and the marker site contains a specific reaction with the sample antigen. Labeled antibodies labeled with signal particles, T-lines are immobilized with capture antibodies that undergo a double-antibody sandwich reaction with sample antigens and labeled antibodies. The capture antibodies are mixed with two antibodies with different binding abilities to sample antigens, and C-lines are fixed An antigen-antibody conjugate that undergoes a double-antibody sandwich reaction with a labeled antibody. The capture antibody on the T line is mixed with two antibodies with different binding abilities, which delays the occurrence of the HOOK effect. The directional coating method is used on the C line. For a higher concentration of the sample to be tested, the labeled antibody competes with the sample antigen. Antigen-antibody conjugates can show a more significant competition gradient, broaden the range of C-line decline, and expand the quantitative detection range of macromolecular proteins.
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Description

Technical field

[0001] The present invention belongs to the field of in vitro diagnostic and immunoassay technology, in particular to a kit and detection method that extends the scope of quantitative detection. Background

[0002] Immunoassay is an analytical method that uses the specific binding reaction of antigens and antibodies to detect the presence or concentration of drugs, hormones, proteins, microorganisms and other substances in complex samples. Depending on the response mechanism, immunoassays can be divided into competition law and non-competition law. Among them, the non-competitive method is to fully react the antigen to be measured with sufficient labeled antibodies to form an antigen-labeled antibody conjugate, and the signal intensity generated is proportional to the amount of antigen. The competition method is to combine the antigen to be measured with the quantitative labeled antigen (direct competition) or the fixed antigen (indirect competition) to form a qua...

Claims

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