Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kit and detection method for expanding the quantitative detection range

A technology of quantitative detection and detection method, applied in the field of in vitro diagnosis and immunoassay, can solve the problem of narrow quantitative detection range of macromolecular protein, and achieve the effect of delaying the HOOK effect, overcoming the influence and simple operation.

Active Publication Date: 2022-06-07
天津中新科炬生物制药股份有限公司
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art and provide a kit and a detection method that expands the quantitative detection range, so as to overcome the problem that the existing macromolecular protein quantitative detection range is narrow

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kit and detection method for expanding the quantitative detection range
  • A kit and detection method for expanding the quantitative detection range
  • A kit and detection method for expanding the quantitative detection range

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: The test kit of the present invention is used to detect the HCG antigen concentration in the sample

[0044] 1. Materials and instruments

[0045] The sample concentration ranged from 10-200000 mIU / mL, and the sample number was N1-N13 in 13 groups of HCG antigen standard samples (diluted from HCG antigen, the specific concentration is shown in Table 1).

[0046] F10Pro type fluorescence immunoassay analyzer, timer (such as stopwatch) and pipette produced by Tianjin Zhongxin Keju Biopharmaceutical Co., Ltd.

[0047] According to the improved technical scheme of the present invention prepared for detecting the concentration of HCG antigen microfluidic chip (said chip comprises a mutually fastening cover sheet and a negative, the cover sheet is provided with a dosing hole, a microchannel and a waste liquid tank along the direction of the sample flow, the region of the negative is directly opposite the microchannel along the sample flow direction in turn set with a l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention proposes a kit and a detection method for expanding the range of quantitative detection, including a carrier, the carrier is provided with a marker site, a T line, and a C line, and the marker site contains a specific reaction with the sample antigen. Labeled antibodies labeled with signal particles, T-lines are immobilized with capture antibodies that undergo a double-antibody sandwich reaction with sample antigens and labeled antibodies. The capture antibodies are mixed with two antibodies with different binding abilities to sample antigens, and C-lines are fixed An antigen-antibody conjugate that undergoes a double-antibody sandwich reaction with a labeled antibody. The capture antibody on the T line is mixed with two antibodies with different binding abilities, which delays the occurrence of the HOOK effect. The directional coating method is used on the C line. For a higher concentration of the sample to be tested, the labeled antibody competes with the sample antigen. Antigen-antibody conjugates can show a more significant competition gradient, broaden the range of C-line decline, and expand the quantitative detection range of macromolecular proteins.

Description

Technical field [0001] The present invention belongs to the field of in vitro diagnostic and immunoassay technology, in particular to a kit and detection method that extends the scope of quantitative detection. Background [0002] Immunoassay is an analytical method that uses the specific binding reaction of antigens and antibodies to detect the presence or concentration of drugs, hormones, proteins, microorganisms and other substances in complex samples. Depending on the response mechanism, immunoassays can be divided into competition law and non-competition law. Among them, the non-competitive method is to fully react the antigen to be measured with sufficient labeled antibodies to form an antigen-labeled antibody conjugate, and the signal intensity generated is proportional to the amount of antigen. The competition method is to combine the antigen to be measured with the quantitative labeled antigen (direct competition) or the fixed antigen (indirect competition) to form a qua...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/76G01N33/577G01N33/558B01L3/00
Inventor 李昀地王俊水李慧许人茂周洪锐魏华英刘钟泉赵娜张粲肖福磊秦月李轩贺敬文刘陈针玉钱龙李娜孟佳李洲
Owner 天津中新科炬生物制药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com