Pleurotus eryngii strain rejuvenation screening method

A screening method, the technology of Pleurotus eryngii, applied in the field of agricultural biology, can solve the problems of poor growth of mycelia, insufficient nutrition in the medium, and failure to inhibit and kill viruses, so as to achieve rich nutrition, short cycle, The effect of strain rejuvenation

Pending Publication Date: 2022-04-22
ANHUI WUYUE FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a screening method for the rejuvenation of Pleurotus eryngii strains, aiming to solve the problem that the current screening method cannot inhibit and kill the virus; The problem of poor growth

Method used

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  • Pleurotus eryngii strain rejuvenation screening method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The raw materials of the first medium: 15 parts of glucose, 3 parts of yeast powder, 100 parts of potatoes, 3 parts of peptone and 0.3 parts of cytokinin.

[0034] Second medium raw materials: 18 parts of glucose, 7 parts of yeast powder, 180 parts of potatoes, 5 parts of sucrose and 10 parts of shell powder.

[0035] The third medium raw material: 30 parts of corn cob, 30 parts of rice bran, 10 parts of bran and 1 part of light calcium carbonate.

[0036] Raw materials for the fourth medium: 10 parts of astragalus, 1 part of Sophora flavescens, 1 part of Jiulixiang, 5 parts of Viola chinensis, 150 parts of potatoes and 30 parts of Coptidis Oral Liquid.

[0037] The preparation method of the first culture medium: specifically comprise the following steps:

[0038] (1) Potato slices, add 3-4 times the amount of water, boil for 20-30 minutes, filter, add 3-4 times the amount of water to the filter residue, continue heating to 50-60°C to obtain product A;

[0039] (2) Gl...

Embodiment 2

[0060] The raw materials of the first medium: 20 parts of glucose, 4 parts of yeast powder, 110 parts of potatoes, 4 parts of peptone and 0.4 parts of cytokinin.

[0061] Second medium raw materials: 20 parts of glucose, 8.5 parts of yeast powder, 190 parts of potatoes, 7.5 parts of sucrose and 12.5 parts of shell powder.

[0062] The raw material of the third medium: 40 parts of corn cob, 40 parts of rice bran, 15 parts of bran and 2.5 parts of light calcium carbonate.

[0063] Raw materials for the fourth medium: 15 parts of astragalus, 5 parts of sophora flavescens, 5 parts of jiulixiang, 10 parts of viola chinensis, 150 parts of potatoes and 30 parts of coptis oral liquid.

[0064] The preparation method of the first culture medium: specifically comprise the following steps:

[0065] (1) Potato slices, add 3-4 times the amount of water, boil for 20-30 minutes, filter, add 3-4 times the amount of water to the filter residue, continue heating to 50-60°C to obtain product A;...

Embodiment 3

[0087] The raw materials of the first medium: 25 parts of glucose, 5 parts of yeast powder, 120 parts of potatoes, 5 parts of peptone and 0.5 parts of cytokinin.

[0088] Second medium raw materials: 22 parts of glucose, 10 parts of yeast powder, 200 parts of potatoes, 10 parts of sucrose and 15 parts of shell powder.

[0089] The raw material of the third medium: 50 parts of corn cob, 40 parts of rice bran, 20 parts of bran and 5 parts of light calcium carbonate.

[0090] Raw materials for the fourth medium: 20 parts of astragalus, 10 parts of sophora flavescens, 10 parts of jiulixiang, 15 parts of viola chinensis, 150 parts of potatoes and 30 parts of coptis oral liquid.

[0091] The preparation method of the first culture medium: specifically comprise the following steps:

[0092] (1) Potato slices, add 3-4 times the amount of water, boil for 20-30 minutes, filter, add 3-4 times the amount of water to the filter residue, continue heating to 50-60°C to obtain product A;

...

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Abstract

The invention is applicable to the technical field of agricultural biology, and provides a pleurotus eryngii strain rejuvenation screening method which comprises the following steps: inoculating pleurotus eryngii strains needing to be purified and rejuvenated into a first culture medium; when the diameter of the bacterial colony inoculated into the first culture medium reaches 1.5 cm, cutting the tip by 1.2 mm, and transplanting into a second culture medium; when the diameter of the bacterial colony inoculated into the second culture medium reaches 1.5 cm, cutting the tip by 1.2 mm, and transplanting into a third culture medium; when the diameter of the bacterial colony inoculated into the third culture medium reaches 1.5 cm, cutting the tip by 1.2 mm, and transplanting into a fourth culture medium; culturing the bacterial colony inoculated into the fourth culture medium at 30 DEG C, and performing tissue isolation on the cultured sporocarp to realize purification and rejuvenation of the pleurotus eryngii strain; according to the method, colony tip cutting is performed for three times, detoxification and purification are performed simultaneously, the period is short, and the efficiency is high.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a screening method for the rejuvenation of Pleurotus eryngii strains. Background technique [0002] Pleurotus eryngii is a typical subtropical grassland-a wild edible fungus in arid desert areas. It is saprophytic in late spring to early summer and facultatively parasitic on the roots and surrounding soil of large umbelliferous plants such as eryngie, ferulae, and lather middle. Pleurotus eryngii is a kind of high-quality large-scale fleshy toadstool in the mountains, grasslands, and deserts of southern Europe, northern Africa, and Central Asia. Agaricaceae, Pleurotaceae, Pleurotus genus. [0003] The current screening method can not inhibit and kill the virus; on the other hand, the current culture medium is not nutritious enough, which makes the growth of the mycelium poor during the rejuvenation process. Contents of the invention [0004] The inventio...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/20A01G18/00
Inventor许腾龙许忠李娟金媛媛王继红汤静许安邵丽张琴奚夏丽
OwnerANHUI WUYUE FOOD