Method for creating mutation of rice seedlings through heavy ion beam radiation
A heavy ion beam and ion beam technology are applied in the field of mutation creation of rice seedlings irradiated by heavy ion beams, which can solve problems such as single sample state, and achieve the effect of increasing the number of plants.
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Embodiment 1
[0041] Embodiment 1, a method for creating mutations of rice seedlings irradiated by heavy ion beams, comprising the following steps:
[0042] (1) Seed disinfection: Select the same batch of rice variety Kitaake (Oryzasativa ssp japonica) without pests and diseases, with the same plumpness, and place its dry seeds in a hydrogen peroxide solution with a mass concentration of 3%, and disinfect them with Rinse with deionized water 4 times;
[0043] (2) Seedling preparation: place the sterilized rice seeds in a water-moistened petri dish and cultivate them for 5 days; germinate in a constant temperature incubator (32°C) without light from day 1 to day 4, and then move to Light culture room (temperature is 28-30°C, photoperiod is 14 hours light, 10 hours dark, light intensity is 250 μmol / m 2 Grow in s) for 1 day to obtain rice seedlings to be irradiated;
[0044] (3) radiation treatment: the rice seedling material obtained in the step (2) is placed in a water-moistened petri dish...
Embodiment 2
[0056] Embodiment 2, a method for creating mutations of rice seedlings irradiated by heavy ion beams, comprising the following steps:
[0057] (1) Seed disinfection: Select the same batch of rice variety Kitaake (Oryzasativa ssp japonica) without pests and diseases, with the same plumpness, and place its dry seeds in a hydrogen peroxide solution with a mass concentration of 3%, and disinfect them with Rinse with deionized water 5 times.
[0058] (2) Seedling preparation: place the sterilized rice seeds in a water-moistened petri dish and cultivate them for 5 days; germinate in a constant temperature incubator (32°C) without light from day 1 to day 4, and then move to Light culture room (temperature is 28-30°C, photoperiod is 14 hours light, 10 hours dark, light intensity is 250 μmol / m 2 s) for 1 day to obtain rice seedlings to be irradiated.
[0059] (3) Radiation treatment: the rice seedling material obtained in the step (2) is placed in a water-moistened petri dish, and th...
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