Molecular marker closely linked with stripe rust resistant genes QYrsv and swst-1BL in adult-plant stage and application of molecular marker

A qyrsv.swust-1bl, molecular marker technology, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of low throughput, hindering high-density genetic maps, and unfavorable development.

Active Publication Date: 2022-04-29
SOUTHWEAT UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these molecular markers have low throughput, are inconvenient to use, and have relatively low efficiency, which is very unfavorable...

Method used

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  • Molecular marker closely linked with stripe rust resistant genes QYrsv and swst-1BL in adult-plant stage and application of molecular marker
  • Molecular marker closely linked with stripe rust resistant genes QYrsv and swst-1BL in adult-plant stage and application of molecular marker
  • Molecular marker closely linked with stripe rust resistant genes QYrsv and swst-1BL in adult-plant stage and application of molecular marker

Examples

Experimental program
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Effect test

experiment example 1

[0016] Experimental example 1 The first round of KASP marker development of durum wheat stripe rust resistance gene QYrsv.swust-1BL

[0017] (1) Determine hybrid combinations to construct fine-mapping populations

[0018] The test materials were wild emmer wheat Zavitan, national approved variety Mianmai 37 (MM 37) and durum wheat Svevo (T.turgidum ssp.durum), the donor material of stripe rust resistance gene QYrsv.swust-1BL. Highly susceptible cultivars Mingxian 169 (MX 169) and Jinmai 47 (JM 47) were used as susceptible controls. Wild emmer wheat Zavitan (T.turgidumssp.dicoccoides) belongs to wild relatives, which are an important source of genetic diversity of hexaploid wheat (Mu Jingmei, 2019). When resistance genes are difficult to find in common wheat varieties, resistance genes in wild relatives can be explored. Many disease resistance genes found in common wheat relatives or wild relatives such as Yr24 / Yr26, Yr53, Yr56, Yr64, Yr65 from T. turgidum and Yr15, Yr35 and ...

experiment example 2

[0044] Experimental example 2 The second round of KASP marker development of durum wheat stripe rust resistance gene QYrsv.swust-1BL

[0045] SNP genotyping of parents Svevo, Zavitan and MM37 using wheat 660K chip. From 630,517 660K chip scan results, there are 66 polymorphic SNPs, compared to the Svevo reference genome, and found that these 66 SNPs are distributed in the interval 661,593,990~663,334,719bp on QYrsv.swust-1BL; Among them, there were 57 polymorphic SNPs in the interval 670,783,568-672,779,761 bp; 42 differential SNPs overlapped between the two reference genomes.

[0046] Based on the SNPs that overlapped in the target interval twice, their flanking sequences were compared to the wheat reference genome, and the physical location information was obtained. Four specific SNPs were successfully developed in the target interval 670,783,568-672,779,761bp and between Svevo and Zavitan The polymorphic KASP markers were A012891, A012893, A012894, A012896 (Table 4), and s...

experiment example 3

[0047] EXPERIMENTAL EXAMPLE 3 Screening of Recombinant Exchange Strains of Durum Wheat Stripe Rust Resistance Gene QYrsv.swust-1BL and Construction of High-Density Genetic Map

[0048] After two rounds of KASP marker development, a total of 13 KASP markers were polymorphic between parents and RIL populations, which could be successfully typed (9 in the first round and 4 in the second round). These markers were used to screen Svevo / Zavitan RILs, A total of 32 recombination exchange strains were obtained, 11 recombination events were closely linked to markers A007972, A012891, A012893, A012894, A012896, and 6 recombination events occurred between markers A007972 and A012896 (see Table 4). The closely linked markers at both ends of QYrsv.swust-1BL are A007972 and A012896, compared to the Chinese spring wheat genome version 1.0, the target interval is shortened to 1550.246kb (physical distance: 670,783,618~672,333,864bp); compared to the Svevo reference genome, the target interval ...

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Abstract

The invention discloses a molecular marker closely linked with an adult-plant-stage stripe rust resistant gene QYrsv.swst-1BL and application of the molecular marker. The stripe rust resistant gene is positioned in sections of long-arm physical sites 662, 307, 854-662, 550 and 261bp of chromosome 1B of a reference genome of durum wheat Svevo; the molecular marker is closely linked with a stripe rust resistant gene QYrsv.swst-1BL, and the molecular marker is A012893 and A012894; the nucleotide sequences of the primers of the molecular marker A012893 are as shown in SEQ ID NO. 36 to SEQ ID NO. 38; the nucleotide sequences of the primers of the molecular marker A012894 are as shown in SEQ ID NO. 39 to SEQ ID NO. 41. The molecular marker A012894 has the advantages that the primer sequence is simple; the anti-stripe rust gene QYrsv.swst-1BL positioned by the invention can be used for improving the resistance of a wheat variety to the wheat stripe rust; the molecular marker closely linked with the anti-disease gene can be used for evaluating the application of the gene in the long-lasting resistance breeding of the stripe rust disease by using marker-assisted selection.

Description

technical field [0001] The invention relates to molecular markers closely linked to genes, in particular to a closely linked molecular marker and application of an adult plant stage anti-stripe rust gene QYrsv.swust-1BL. Background technique [0002] Wheat stripe rust is one of the most serious fungal diseases threatening wheat production. Combined with DNA molecular marker technology to mine and locate stripe rust resistance genes, compared with conventional hybrid breeding, previous cytogenetic chromosome banding technology and isoenzyme technology, it makes gene mapping research more convenient and faster, and accelerates the development of disease-resistant varieties. Nurturing process. [0003] With the development of new molecular markers and the application of high-throughput next-generation sequencing technologies in recent years, single nuclotide polymorphism (SNP) markers have the advantages of wide distribution in the genome, high genetic stability, and low singl...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/6895C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周新力李鑫方桃红张敏郑晓晨王玉琪田冉苟兆霞李可心杨随庄康振生
Owner SOUTHWEAT UNIV OF SCI & TECH
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