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Kit and method for determining multiple estrogens in dried blood spot

A technology of estrogen and dried blood, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection sensitivity and low ionization efficiency, and achieve improved analysis efficiency, easy storage and transportation, and increased throughput. volume effect

Active Publication Date: 2022-04-29
HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the prior art, estrogen exists at trace levels in biological samples and human bodies, and the content in dried blood film samples is extremely low, and estrogen compounds are less polar, and the ionization efficiency in ESI and APCI sources in mass spectrometry is not high , the technical problem of low direct detection sensitivity, this application provides a kit for the determination of various estrogens in dried blood slices, the extraction and derivatization pretreatment combined with ultra-high performance liquid chromatography tandem mass spectrometry can quickly, easily and accurately detect Trace amounts of estrogen in blood film

Method used

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  • Kit and method for determining multiple estrogens in dried blood spot
  • Kit and method for determining multiple estrogens in dried blood spot
  • Kit and method for determining multiple estrogens in dried blood spot

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Effect test

Embodiment 1

[0034] A kind of test kit for measuring multiple estrogens in dried blood slices of the present embodiment, said test kit includes the following components:

[0035] Table 1 Components of kits for the determination of various estrogens in dried blood slices

[0036]

[0037] In this embodiment, the calibrator of component 1 includes estrone, estradiol, and estriol; the internal standard includes estrone-d4, estradiol-d3, and estriol-d3.

[0038] The concentration of dansyl chloride in component 4 was 2 mg / mL in 100 μL.

[0039] Na of component 5 2 CO 3 The buffer solution has a concentration of 100 mmol and a pH of 10.5.

[0040] The first extractant of component 6 is 30 mL.

[0041] The second extractant for component 7 was 150 mL.

[0042] The kit of this embodiment requires less blood collection, and the samples are easy to collect, and are easier to store and transport than liquid samples such as serum. It can be used to detect the concentration of estrone, estrad...

Embodiment 2

[0044] A kit for determining multiple estrogens in dried blood slices in this embodiment is basically the same as that in Embodiment 1, except that the derivatization reagent is N-trimethylsilyl imidazole.

[0045] Table 2 Components of kits for the determination of various estrogens in dried blood slices

[0046]

Embodiment 3

[0048] A kit for determining various estrogens in dried blood slices in this embodiment is basically the same as that in Embodiment 1, except that the derivatization reagent is pentafluorobenzoyl chloride.

[0049] Table 3 Components of kits for determination of various estrogens in dried blood slices

[0050]

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Abstract

The invention belongs to the technical field of hormone detection, and relates to a kit and a method for determining various estrogens in dried blood spots. Aiming at the technical problems that in the prior art, estrogen exists at a trace level in a biological sample and a human body, the content of estrogen in a dried blood spot sample is extremely low, the polarity of estrogen compounds is relatively small, the ionization efficiency in ESI and APCI sources in a mass spectrum is not high, and the direct detection sensitivity is not high, the invention provides a kit for determining various estrogens in a dried blood spot. Comprising a derivatization reagent, a first extraction agent and a second extraction agent, and the trace estrogen in the dried blood spot can be rapidly, simply and accurately detected by combining extraction and derivatization pretreatment with ultra-high performance liquid chromatography-tandem mass spectrometry. The invention further provides a method for determining the multiple estrogens in the dried blood spot, the estrogens are extracted from the dried blood spot by using the first extraction agent and then derivatized, and the derived estrogens are extracted again by using the second extraction agent, so that the flux is increased, and the analysis efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of hormone detection, and in particular relates to a kit and a method for measuring multiple estrogens in dried blood slices. Background technique [0002] Estrogen is an important sex hormone in women and has important and extensive physiological functions. Studies have shown that estrogen not only has the physiological function of maintaining female reproductive function and secondary sexual characteristics, but also regulates the metabolism of the human body, the development of the nervous system, and the development of bones. Estrogen mainly includes three kinds of estrone (E1), estradiol (E2) and estriol (E3). The level of estrone in the human body is an important indicator for evaluating precocious puberty and bone risk assessment for the elderly. Estradiol is the most biologically active estrogen in women. Estriol is the metabolite of estradiol and estrone, the source of the precursor In the fetal ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 彭军刘超
Owner HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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