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Medicine and method for antagonizing porcine respiratory and reproductive syndrome virus replication

A virus replication and pig breathing technology, applied in the field of medicine, can solve the problems of infection with porcine reproductive and respiratory syndrome virus, etc., and achieve the effect of alleviating interstitial pneumonia, clear medicinal components and controllable quality.

Pending Publication Date: 2022-05-13
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a medicine and method for antagonizing the replication of porcine respiratory and reproductive syndrome virus, which solves the problem of pig infection with porcine reproductive and reproductive syndrome virus, can effectively antagonize the replication of porcine respiratory and reproductive syndrome virus, and reduce infection Significantly reduced lung inflammation and pathological damage in pigs with clinical symptoms

Method used

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  • Medicine and method for antagonizing porcine respiratory and reproductive syndrome virus replication
  • Medicine and method for antagonizing porcine respiratory and reproductive syndrome virus replication
  • Medicine and method for antagonizing porcine respiratory and reproductive syndrome virus replication

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1DI

[0025] Inhibition of Experimental Example 1 DIC to PRRSV on cells

[0026] 1. Detection of the inhibitory effect of DIC on PRRSV in PAM cells

[0027] (1)CC 50 Determination of

[0028] The cytotoxicity of DIC to PAM cells was first determined by the CCK8 cytotoxicity assay (CC 50 value), as follows:

[0029] PAM cells were cultured with 10% FBS-DMEM containing different concentrations of DIC (1-250 μM), incubated at 37°C for 48 hours, cell viability was detected with an enhanced CCK8 kit, and the time required for 50% of the cells to produce cytotoxicity was calculated using GraphPad Prism 7.0 software. Concentration (CC 50 ), and DMSO was used as a negative control.

[0030] The test results are as follows:

[0031] Such as figure 1 As shown in A, DIC will produce 50% cytotoxicity (CC 50 value).

[0032] (2)IC 50 Determination of

[0033] The inhibitory efficiency of DIC to PRRSV on PAM cells was detected by indirect immunofluorescence, as follows:

[0034] Add d...

experiment example 2DI

[0059] Experimental example 2 Therapeutic effect of DIC on PRRSV infected piglets

[0060] 1. Experimental Design of Piglet Treatment

[0061] Twenty 5-week-old commercial piglets were detected by ELISA and PCR respectively, and the antibodies and antigens of PRRSV, PCV2, PRV and CSFV were all negative. Randomly divided into 4 groups (5 in each group), specifically:

[0062] The first group is a positive control group (Vehicle group) of PRRSV infection and normal saline+0.5% DMSO (ie 0.5% DMSO normal saline solution);

[0063] The second group is PRRSV infection and DIC 5mg / kg treatment group;

[0064] The third group is PRRSV infection and DIC 15mg / kg treatment group;

[0065] The fourth group is the uninfected and untreated negative control group.

[0066] The above-mentioned PRRSV infection was piglet intranasal injection and intramuscular injection of 1 mL PRRSV BB0907 strain (3*10 5 TCID 50 ), infection 24h, intramuscular injection of 5, 15mg / kg DIC or 0.5% DMSO res...

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Abstract

The invention discloses a drug and a method for antagonizing porcine respiratory and reproductive syndrome virus replication, the drug is biscoumarin, the invention discloses that DIC has anti-PRRSV activity, the DIC is used for inhibiting PRRSV replication in PRRSV natural target cells PAM cells, the IC50 value is very low, and the DIC shows the potential for treating PRRSV infection in the PAM cells for the first time. In a piglet treatment experiment, the DIC can relieve the clinical symptoms such as anorexia, fever, sleepiness, depression and dyspnea caused by the PRRSV; viremia, lung virus load and intranasal detoxification amount of infected piglets are greatly reduced; the interstitial pneumonia caused by the PRRSV is relieved, and the enormous potential of serving as a PRRSV treatment medicine is shown.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a medicine and a method for antagonizing the replication of porcine respiratory and reproductive syndrome virus. Background technique [0002] The pathogen of porcine reproductive and respiratory syndrome is porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV), which belongs to Arteriviridae and is a single-stranded positive-sense RNA virus. The virus infection can cause reproductive disorders in pregnant sows, respiratory system disorders in pigs of all ages, immunosuppression in pigs, and diseases that promote secondary infections, causing huge economic losses to the pig breeding industry at home and abroad. [0003] However, commercial vaccines are not yet ideal, and the cross-protection ability of vaccines against virus strains is not complete, and the process of live virus replication in pigs has the possibility ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P31/14
CPCA61K31/352A61P31/14
Inventor 刘雪威李焕荣周双海王君君李倬伟陈祯涵
Owner BEIJING UNIV OF AGRI
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