Plant methyl jasmonate in-situ detection method based on plate electrode biosensor

Abstract

The invention provides a plant methyl jasmonate in-situ detection method based on a plate electrode biosensor, which realizes in-situ online detection of methyl jasmonate in a plant through the plate electrode biosensor. Compared with the traditional mass spectrum chromatography, the method disclosed by the invention does not need in vitro, has no essential harm to plants, has the characteristics of simplicity in operation, high sensitivity, high accuracy, convenience in carrying and the like, can realize dynamic acquisition of jasmonic acid methyl ester at different parts of plant leaves, and provides technical support for research of jasmonic acid methyl ester in plants.

Description

technical field [0001] The invention relates to a biological detection method, in particular to an in-situ detection method of plant methyl jasmonate based on a flat electrode biosensor. Background technique [0002] Methyl jasmonate (MeJA) is a plant endogenous hormone, which acts as a growth regulator, regulates the entire growth and development process of plants from seed germination to senescence, and also acts as a defense signaling molecule that activates plant stress responses. In agricultural production, exogenous application of MeJA can promote the synthesis of anthocyanins and phenolic compounds in fruits, which helps to improve the color and antioxidant capacity of fruits. In addition, MeJA treatment can also alleviate the adverse effects of agricultural products caused by mechanical damage during transportation or storage after harvest. However, due to the low content of MeJA in plants, usually between 0.01-1500 ng / g, there is an urgent need for a simple, sensit...

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Problems solved by technology

[0003] At present, the quantitative detection methods of MeJA include enzyme-linked immunosorbent assay (ELISA), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), electrochemical methods, etc.; all of the above methods need to collect plant samples for separation and purification , then testing will not only cause damage to the plant, but also the results cannot accurately reflect the real-time changes in the content of MeJA in the plant

Electrochemical immunosensor is an electrochemical sensing technology based on antigen-antibody specific interaction, which combines the selectivity of antibodies and the sensitivity of electrochemical techniques, and has the advantages of portability, low cost, and easy miniaturization. In the prior art, there is no mature method or process for applying biosensors to the quantitative detection of MeJA in plants, and there are no relevant literature reports

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