Method for efficiently producing cymbidium sinense tissue culture seedlings

A high-efficiency technology of Molan group, applied in the field of plant tissue culture, can solve the problem of low production efficiency of Molan tissue culture seedlings, and achieve the effect of commercialized high-efficiency production, obvious production advantages, and high production efficiency

Active Publication Date: 2022-05-24
肇庆雅兰芳农业科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the production efficiency of molantium tissue culture seedlings is not high at present, the present invention proposes an innovative way of double culture medium and double culture mode, which can ensure the production efficiency of molantium group The requirements of different hormones, nutrients and culture conditions for the propagation materials at each stage of the seedling regeneration process, and the use of the solid-liquid two-layer culture method not only avoids the shortcomings of the single culture method, but also makes full use of the advantages of the two culture methods to meet the requirements of reproduction. On the basis of the material's demand for oxygen, the effective and rapid absorption and utilization of rich nutrients and growth regulators in both solid and liquid media can achieve the cultivation goals of each stage. The production efficiency is higher than the literature reports, which is beneficial to the tissue culture of Molan The promotion and planting of seedlings has the characteristics of low cost and high benefit, and it is an effective way to promote the production and application of molan tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Material: Wu Zicui.

[0033] 2. Selection and sterilization of explants: Select a healthy flowering Molan with vigorous growth and no pests and diseases as the mother plant. After artificial pollination, each mother plant will pollinate two flowers. During the fruit development stage, pay attention to control the suitable mother plant. The light, temperature and humidity conditions for plant growth, strengthen its fertilizer and water management and control of pests and diseases, so that the fruit is fully developed, and the capsule can be cut 180 days after pollination for disinfection and inoculation. , and then disinfected with 0.1% mercuric chloride solution for 15 minutes, and finally rinsed with sterile water for 5 times. The capsules washed and washed with sterile water were placed on sterilized filter paper to absorb water, the capsules were cut with a scalpel, and the seeds were evenly sown into the rhizome induction medium M1.

[0034] 3. Induction of rhiz...

Embodiment 2

[0040] This example is basically the same as Example 1, but has the following differences: the experimental material is white ink tissue culture seedlings, and the immersion time of the root-extracting vitality agent (an aqueous solution containing mass fraction of 0.2% potassium permanganate and 0.6% sodium chloride) is 20 minutes ; M1 composition in this example: Huabao No. 1 2.0g / L+ Huabao No. 2 2.0g / L+ banana 80g / L+ apple 20g / L+1.0mg / L 6-BA+1.0mg / LNAA+20g / L sucrose +Activated carbon 2.0g / L+5.0g / L agar, pH 5.4; M2 ingredients: Huabao No. 1 1.5g / L+ Huabao No. 2 1.5g / L+ Banana 80g / L+ Apple 20g / L+0.5mg / L 6- BA+2.0mg / LNAA+20g / L sucrose+activated carbon 2.0g / L+5.0g / L agar, pH 5.4; M3 component: MS+0.5mg / L6-BA+2.0mg / LNAA+20g / L sucrose, pH 5.4; M4 ingredients: Huabao No. 1 1.5g / L + Huabao No. 2 1.5g / L + banana 80g / L + apple 20g / L + 1.0mg / LTDZ + 0.5mg / L NAA + 20g / L sucrose + 5.0g / L Agar, pH 5.6; M5 composition: MS+1.0mg / L TDZ+0.5mg / LNAA+20g / L sucrose, pH 5.6; M6 composition: Huaba...

Embodiment 3

[0042] This example is basically the same as Example 1, except for the following differences: the experimental material is Huizhou Molan tissue culture seedlings, and the root-lifting activity agent (containing an aqueous solution of 0.15% potassium permanganate and 0.4% sodium chloride in mass fraction) soaking time It is 30min; M1 component in this example: Huabao No. 1 1.5g / L+ Huabao No. 2 1.5g / L+ banana 50g / L+ apple 10g / L+0.5mg / L 6-BA+0.5mg / L NAA+15g / L sucrose+activated carbon 0.5g / L+5.0g / L agar, pH 5.2. M2 component in this example: Huabao No. 1 1.0g / L + Huabao No. 2 1.0g / L+ banana 50g / L+ apple 10g / L+0.25mg / L 6-BA+1.0mg / LNAA+15g / L sucrose+ Activated carbon 1.0g / L+5.0g / L agar, pH 5.2; M3 composition: 1 / 2MS+0.25mg / L 6-BA+1.0mg / LNAA+15g / L sucrose, pH 5.2. M4 component in this example: Huabao No. 1 1.0g / L + Huabao No. 2 1.0g / L + banana 50g / L+ apple 10g / L+0.5mg / LTDZ+0.2mg / LNAA+15g / L sucrose+5.0g / L agar, pH 5.4; M5 composition: 1 / 2MS+0.5mg / L TDZ+0.2mg / LNAA+15g / L sucrose, pH...

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Abstract

The invention discloses a method for efficiently producing cymbidium sinense tissue culture seedlings, and belongs to the field of plant tissue culture. According to the method disclosed by the invention, through a double-culture medium and double-culture mode, the requirements of propagation materials for different hormones, nutrients and culture conditions in each stage of a cymbidium sinense tissue culture seedling regeneration process can be ensured, and the advantages of the two culture modes are fully utilized by utilizing a solid-liquid two-layer culture mode; on the basis of meeting the requirement of a propagation material for oxygen, the culture target of each stage is achieved through effective and rapid absorption and utilization of rich nutritional ingredients of the solid culture medium and the liquid culture medium and the growth regulator, so that the production efficiency is higher than that reported in literatures, popularization and planting of cymbidium sinense tissue culture seedlings are facilitated, and the method has the advantages of being low in cost and high in benefit.

Description

Technical field: [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for efficiently producing Molan tissue culture seedlings. Background technique: [0002] Cymbidium sinense, also known as the orchid, is a plant of the Orchidaceae (Orchidaceae) genus Cymbidium, native to my country, Vietnam and Myanmar. Because of its flowering period in the Spring Festival, it is deeply loved by the Chinese and has high economic value. , ornamental value and medicinal value. The common methods of ramet propagation and tissue culture propagation of Moran are. The traditional way of reproduction is mainly ramet reproduction, but faced with problems such as low reproduction rate, long cycle, infectious diseases and insect pests, and susceptibility to viral diseases that lead to the degeneration of species, it is impossible to achieve mass production and meet the needs of the market. With the development of tissue culture technology, schol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02P60/40Y02A40/22
Inventor 吴坤林潘启明高永林邵紫洋陈肖霞潘伟
Owner 肇庆雅兰芳农业科技有限公司
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