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Application of substance for knocking down BACE2 gene expression in tumor resistance and BACE2 inhibitor

A gene expression and anti-tumor technology, applied in the field of biomedicine, can solve the problems of low cure rate and poor pertinence, achieve good therapeutic effect, inhibit proliferation, and have good application prospects

Pending Publication Date: 2022-05-31
湖北亿粲健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, surgical treatment has the disadvantages of poor pertinence and low cure rate.

Method used

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  • Application of substance for knocking down BACE2 gene expression in tumor resistance and BACE2 inhibitor
  • Application of substance for knocking down BACE2 gene expression in tumor resistance and BACE2 inhibitor
  • Application of substance for knocking down BACE2 gene expression in tumor resistance and BACE2 inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

The transfection solution was configured according to Table 1. After the cells were transfected for 8 h, the normal medium was replaced, and the culture was continued for 48 h.

2. the six-hole plate is placed on ice, and the isolated protein sample is extracted, and with GAPDH as an internal reference, the detection of the

BACE2, Bcl-2, GAPDH protein expression levels.

Table 1

[0089]

Group DMEM Medium Lip6000si‑RNA Solution

Embodiment 2

pre-incubated for 24 hours.

2. configure transfection solution according to table 2, the cell after preculture is transfected, after 6 hours of transfection, replace cell

Serum-containing medium was continued for 24 h.

Table 2

[0095]

Group DMEM Medium Lip6000si‑RNA Solution

Normal group 0.2ml‑‑

Embodiment 3

pre-incubated for 24 hours.

3. The precultured cells are transfected with transfection solution (same as Example 1), and after 6 hours of transfection, the cells are more

The serum-containing medium was changed, and the culture was continued for 24 h.

4. use the pipette tip to compare with a ruler after the cell is overgrown, try to be perpendicular to the horizontal line scratch on the back, the pipette tip should be vertical, do not

tilt.

[0103] 5. Wash cells 3 times with PBS, remove streaked cells, and add serum-free DMEM medium. Put into 37℃, 5%

CO

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PUM

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Abstract

The invention provides an application of a substance for knocking down BACE2 gene expression in tumor resistance and a BACE2 inhibitor. The tumor is a colon tumor. According to the invention, the expression of the BACE2 gene is obviously reduced by selecting specific siRNA, and the migration, proliferation and invasion capabilities of tumor cells are effectively inhibited. Therefore, the proliferation of the colon tumor can be effectively inhibited by knocking down the expression of the BACE2 gene, and a new thought is provided for the treatment of the colon tumor.

Description

Application of substances knocking down BACE2 gene expression in anti-tumor and BACE2 inhibitors technical field The present invention relates to the field of biomedicine, be specifically related to the application of the substance that knocks down BACE2 gene expression in antitumor. with BACE2 inhibitors. Background technique Colon tumor is a common malignant tumor in the gastrointestinal tract, and its incidence is second only to gastric and esophageal cancer, and it is the most common cancer Common section. Colorectal cancer is not impossible to prevent. In fact, colorectal cancer is the easiest disease to self-screen. If it can be detected early, it is also The most curable disease. The treatment of colon tumors generally requires surgical resection of the tumor, and at the same time, a tumor adjacent to the tumor should be removed. segment of the bowel and surrounding lymph nodes. However, surgical treatment has the disadvantages of poor targeting and low cur...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/7088A61K31/713A61P35/00A61P1/00
CPCA61K45/00A61K31/7088A61K31/713A61P35/00A61P1/00
Inventor 刘洁阮班来陈紫寅丁千山
Owner 湖北亿粲健康产业有限公司
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