Method for monitoring mRNA (messenger ribonucleic acid) in breast cancer cells based on fluorescence imaging of DNA (deoxyribonucleic acid) five-

A technology of breast cancer cells and nanomaterials, applied in the field of monitoring mRNA in breast cancer cells based on fluorescence imaging of DNA pentagram nanomaterials, can solve the problems of inability to quickly observe the expression of intracellular markers and the detection is not intuitive enough The effects of commercial promotion, wide application range, and simple reaction conditions

Active Publication Date: 2022-06-03
QINGDAO UNIV OF SCI & TECH
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Problems solved by technology

Fluorescence detection is more stable than electrochemiluminescence detection and electrochemical detection, but the detection is not intuitive enough to quickly observe the expression of intracellular markers

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  • Method for monitoring mRNA (messenger ribonucleic acid) in breast cancer cells based on fluorescence imaging of DNA (deoxyribonucleic acid) five-
  • Method for monitoring mRNA (messenger ribonucleic acid) in breast cancer cells based on fluorescence imaging of DNA (deoxyribonucleic acid) five-
  • Method for monitoring mRNA (messenger ribonucleic acid) in breast cancer cells based on fluorescence imaging of DNA (deoxyribonucleic acid) five-

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[0040] The present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the related invention, but not to limit the invention. In addition, it should be noted that, for the convenience of description, only the parts related to the invention are shown in the drawings.

[0041] It should be noted that the embodiments in the present application and the features of the embodiments may be combined with each other in the case of no conflict. The present application will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments. The specific implementation is as follows:

[0042] (1) Synthesis of DNA Pentagram Nanomaterials

[0043] The 5 nucleic acid strands (H1-H5) were treated with 150 mM NaCl, 40 mM Tris, 40 mM C, respectively. 4 H 6 O 4 , Mg 2+ and 1 mM EDTA buffer...

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Abstract

The invention discloses a method for monitoring mRNA (messenger ribonucleic acid) in breast cancer cells based on DNA (deoxyribonucleic acid) five-pointed star nano-material fluorescence imaging, which is characterized by comprising the following steps: S1, synthesizing a DNA five-pointed star nano-material, S2, carrying out fluorescence detection on the content of MMP mRNA, and S3, carrying out fluorescence detection on the content of FN mRNA. And S4, cell imaging. According to the scheme, the mRNA in the breast cancer cells is monitored through fluorescence imaging, the advantages of being high in detection sensitivity, high in detection selectivity and high in intuition are achieved, the tumor marker mRNA in the breast cancer cells can be more accurately detected, and help is provided for early diagnosis of the focus of a tumor patient.

Description

technical field [0001] The invention relates to the field of detection methods for tumor markers for clinical diagnosis of cancer, in particular to a method for monitoring mRNA in breast cancer cells based on DNA pentagram nanomaterial fluorescence imaging. Background technique [0002] Matrix metalloproteinase messenger RNA (MMP mRNA) and fibronectin messenger RNA (FN mRNA), as tumor markers for clinical diagnosis of cancer, are closely related to breast cancer. Determining its content in cells is of great significance to the early intuitive diagnosis of malignant tumors and the evaluation of curative effects. [0003] At present, polymerase chain reaction (PCR) and multiplex real-time quantitative polymerase chain reaction (MRT-PCR) are commonly used in clinic to detect intracellular mRNA content. Among them, the operation process of PCR clinical detection of mRNA is complicated, the method is cumbersome, and there are disadvantages of high detection limit, which is not c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6841
CPCC12Q1/6886C12Q1/6841C12Q2525/205C12Q2525/30C12Q2563/107Y02A50/30
Inventor 纪小婷赵彬宇张馨桐朱佳霖
Owner QINGDAO UNIV OF SCI & TECH
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