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Prognostic risk scoring model for uveal melanoma and application of prognostic risk scoring model

A melanoma and risk scoring technology, applied in the field of uveal melanoma prognostic risk scoring model, which can solve problems such as unclear specific causes

Pending Publication Date: 2022-06-03
THE EYE HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The onset of uveal melanoma may be related to chromosomal mutation and gene change, and may also be transformed from uveal nevus, but the specific reason is still unclear

Method used

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  • Prognostic risk scoring model for uveal melanoma and application of prognostic risk scoring model
  • Prognostic risk scoring model for uveal melanoma and application of prognostic risk scoring model
  • Prognostic risk scoring model for uveal melanoma and application of prognostic risk scoring model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1 The correlation between the expression levels of SLC12A3 and SLC12A9 and the prognosis of UVM patients

[0094] To evaluate the relationship between SLC12s and the prognosis of UVM patients, the present invention performed univariate Cox regression analysis in the TCGA-UVM cohort. The results showed that high expression of SLC12A3 and SLC12A9 was significantly associated with worse overall survival (OS) (SLC12A3: HR=1.16, 95%CI=1.03-1.29, P=0.01; SLC12A9: HR=2.36, 95%CI= 1.14-4.88, P=0.02), and was significantly correlated with disease-specific survival (DSS) (SLC12A3: HR=1.16, 95%CI=1.03-1.31, P=0.01; SL C12A9: HR=2.55 , 95%CI=1.20-5.45, P=0.02), while other SLC12 family members did not show significant correlation (eg figure 1 shown in A).

[0095] Survival analysis also showed that according to the expression levels of SLC12A3 and SLC12A9, 77 UVM patients could be divided into high-risk and low-risk groups with significant differences in OS and DSS (Log-R...

Embodiment 2

[0097] Example 2 The effect of overexpression of SLC12A3 and / or SLC12A9 on the proliferation of uveal melanoma cells

[0098] The present invention adopts immunohistochemical method to analyze the expression of SLC12A3 and SLC12A9 in uveal melanoma tissue. Immunohistochemical staining showed that SLC12A3 and SLC12A9 were lowly expressed in normal retinal paracancerous tissues and highly expressed in uveal melanoma tissues (eg figure 2 shown in A-C). In order to study the functional roles of SLC12A3 and SLC12A9 in uveal melanoma, the present invention utilized lentivirus infection to overexpress SLC12A3 and SLC12A9 in uveal melanoma cell lines C918 and MUM2C. The results of Western blotting showed that the protein levels of SLC12A3 and SLC12A9 were increased in the overexpressed cell lines, respectively (e.g. figure 2 D and figure 2 E shown). The cell growth curves showed that both overexpression of SL C12A3 and SLC12A9 increased cell proliferation activity compared with...

Embodiment 3

[0099] Example 3 Effects of knockdown of SLC12A3 and SLC12A9 on the proliferation of uveal melanoma cells

[0100] The present invention knocks down SLC12A3 or SLC12A9 in the uveal melanoma cell line, or knocks down SLC12A3 and SLC12A9 at the same time, and it is found that si-SLC12A3 (si-A3 for short) and si-SLC12A9 (si-A9 for short) can respectively effectively Knock down SLC12A3 and SLC12A9 (eg image 3 shown in A). Cell growth curves showed that knockdown of SLC12A3 and SLC12A9 inhibited the proliferation of C918 cells (eg image 3 shown in B). Simultaneous knockdown of SLC12A3 and SLC12A9 in C918 cells has a stronger inhibitory effect on cell proliferation (eg image 3 shown in B). To further confirm this result in other uveal melanoma cell lines, the present invention also knocked down SLC12A3 and SLC12A9 in MUM2C cells, and the cell growth curve also showed that the proliferation of SLC12A3 and SLC12A9 knockdown cells was inhibited ( image 3 C). Consistent with t...

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Abstract

The invention discloses a uveal melanoma prognosis risk scoring model and application thereof, and particularly discovers that SLC12A3, SLC12A9, MIR140 and HCP5 are significantly related to the prognosis of a patient with uveal melanoma, the uveal melanoma prognosis risk scoring model is constructed for the first time according to the four genes, and then a risk score is obtained. The clinical result of uveal melanoma can be predicted through risk scoring, and the method has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a uveal melanoma prognosis risk scoring model and its application. Background technique [0002] Biomarkers are defined as biochemical indicators that objectively measure and evaluate normal biological processes, pathological processes, or pharmacological responses to therapeutic interventions. Biomarkers, also known as molecular markers, can be used to see how the body responds to treatment conditions for diseases. Searching for potential biomarkers of cancer is of great significance to cancer research. In recent years, studies have shown that the application of biomarkers in cancer research mainly includes the following three aspects, namely diagnosis, prognosis and prediction. In terms of diagnosis, it is mainly manifested in the early diagnosis of cancer. In terms of prognosis, it refers to predicting the natural history of cancer and whether the patient's prognosis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16H50/30G16H50/70G16B40/00G16B25/20G16B25/10G01N33/68G01N33/574C12Q1/6886
CPCG16H50/30G16H50/70G16B40/00G16B25/20G16B25/10C12Q1/6886G01N33/5743G01N33/57484G01N33/6848C12Q2600/158C12Q2600/118G01N2800/52
Inventor 孙杰闫聪聪周猛麻晓银郑钦象
Owner THE EYE HOSPITAL OF WENZHOU MEDICAL UNIV
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