Method for separating compound by off-line three-dimensional high-speed counter-current chromatography
A high-speed countercurrent chromatography and compound technology, which is applied in separation methods, separation/purification of carbonyl compounds, organic chemical methods, etc., can solve the problems of large sample loss, large solvent consumption, and the separation of effective chemical components of betel leaf by HSCCC. , to increase the productivity
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[0083] 1. Experimental medicinal materials
[0084] Piper betle.L belongs to a climbing plant belonging to the genus Piper of the family Piperaceae. The betel leaf samples were collected from Yunnan Province.
[0085] 2. Experimental reagents
[0086] Petroleum ether, ethyl acetate, methanol, and chloroform used for HSCCC separation were all analytically pure reagents, purchased from Shanghai Sinopharm Chemical Reagent Company, China; HPLC methanol was obtained from CINC High Purity Solvent Company (Shanghai).
[0087] 3. Experimental equipment
[0088] High-speed countercurrent chromatograph (OptiChrome-300plus semi-preparative type), constant flow pump (CF-80), six-port valve, PTFE connecting tube (1.6*2.4mm), injection loop (120ml), multi-layer coil ( Internal diameter: 2.1mm; radius: 80mm) was provided by Jiangyin Countercurrent Chromatography Technology Co., Ltd.; UV detector (UV1001), purchased from Shanghai Sanwei Scientific Instrument Co., Ltd.; Nuclear Magnetic Reson...
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