Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating compound by off-line three-dimensional high-speed counter-current chromatography

A high-speed countercurrent chromatography and compound technology, which is applied in separation methods, separation/purification of carbonyl compounds, organic chemical methods, etc., can solve the problems of large sample loss, large solvent consumption, and the separation of effective chemical components of betel leaf by HSCCC. , to increase the productivity

Pending Publication Date: 2022-06-07
上海致臻志臣科技有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art records that neolignans have synergistic anti-drug-resistant Staphylococcus aureus activity, but due to the structural similarity of these compounds, conventional separation methods are difficult to separate and the sample loss is large (J.Org.Chem.2021,86,16 , 11072-11085 Publication Date: January 13, 2021, https: / / doi.org / 10.1021 / acs.joc.0c02682)
[0004] The study found that there is no report on the use of HSCCC to separate the effective chemical components of betel leaves
In addition, macroporous resin, silica gel, sephadex LH-20 and preparative HPLC are commonly used to separate betel leaves, but the separation method is cumbersome and consumes a lot of solvent, which means high risk of irreversible sample adsorption and low yield of sample recovery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating compound by off-line three-dimensional high-speed counter-current chromatography
  • Method for separating compound by off-line three-dimensional high-speed counter-current chromatography
  • Method for separating compound by off-line three-dimensional high-speed counter-current chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] 1. Experimental medicinal materials

[0084] Piper betle.L belongs to a climbing plant belonging to the genus Piper of the family Piperaceae. The betel leaf samples were collected from Yunnan Province.

[0085] 2. Experimental reagents

[0086] Petroleum ether, ethyl acetate, methanol, and chloroform used for HSCCC separation were all analytically pure reagents, purchased from Shanghai Sinopharm Chemical Reagent Company, China; HPLC methanol was obtained from CINC High Purity Solvent Company (Shanghai).

[0087] 3. Experimental equipment

[0088] High-speed countercurrent chromatograph (OptiChrome-300plus semi-preparative type), constant flow pump (CF-80), six-port valve, PTFE connecting tube (1.6*2.4mm), injection loop (120ml), multi-layer coil ( Internal diameter: 2.1mm; radius: 80mm) was provided by Jiangyin Countercurrent Chromatography Technology Co., Ltd.; UV detector (UV1001), purchased from Shanghai Sanwei Scientific Instrument Co., Ltd.; Nuclear Magnetic Reson...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for off-line three-dimensional high-speed counter-current chromatography separation of compounds. The invention also provides a method for separating the neolignan compound from the betel. According to the off-line three-dimensional high-speed counter-current chromatography compound separation method provided by the invention, the separation effect of a previous solvent system is kept by an in-situ concentration technology, and rapid separation of compounds with similar structures is realized by combining online storage and multi-stage circulating elution.

Description

technical field [0001] The invention relates to the technical field of natural medicines, in particular to a method for separating compounds by off-line three-dimensional high-speed countercurrent chromatography. More specifically, the present invention relates to a method for the separation and purification of compounds of natural origin using off-line three-dimensional high-speed countercurrent chromatography. Background technique [0002] High-speed countercurrent chromatography (HSCCC) was first proposed by Ito et al. in the early 1960s, and then gradually developed. It is a unique solid support-free, liquid-liquid partition chromatography with the ability to eliminate irreversible samples on solid supports. The characteristic of adsorption is that the stationary phase is the mobile phase, which is a commonly used recyclable organic reagent. Compared with traditional separation techniques, HSCCC has the advantages of fast and efficient separation, simple operation, low ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04C07D307/83C07C45/79C07C49/753B01D15/18B01D15/42
CPCC07D407/04C07D307/83C07C45/79B01D15/1807B01D15/424C07B2200/07C07C49/753
Inventor 穆青肖川云田勇田云才
Owner 上海致臻志臣科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products