3-indole substituted phenylborazole compound as well as preparation method and application thereof
A technology of borazole and compound, which is applied in the field of 3-indole substituted phenylborazole and its preparation field, and achieves the effects of good bactericidal activity and broad-spectrum bactericidal activity
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Embodiment 1
[0036] The 3-indole-substituted phenylborazole compounds shown in formula I are synthesized with the o-formylbenzeneboronic acid shown in formula III and the substituted indole shown in formula IV as raw materials. The synthetic route is as follows:
[0037]
[0038] Specific steps are as follows:
[0039] Take a 100 mL round-bottomed flask, add o-formyl phenylboronic acid (2 mmol), substituted indole (3 mmol), and 40 mL of deionized water successively, react at room temperature, and monitor the progress of the reaction by TLC; after the reaction is completed, extract with dichloromethane, add silica gel to mix Sample, eluted with 17%-20% ethyl acetate / petroleum ether as eluent to carry out silica gel column chromatography purification to obtain the target compound, dried and weighed. To calculate the yield, use 1 H NMR, 13 Its structure was characterized by C NMR, IR and MS.
[0040] The obtained 3-indole-substituted phenylborazole compounds are shown in Table 1.
[00...
Embodiment 2
[0081] Detection of bactericidal activity of target compounds
[0082] With strawberry botrytis cinerea (Botrytis cinerea), rice sheath blight (Rhizoctonia solani), tomato early blight (Alternaria solani), wheat scab (Gibberella zeae), cucumber anthracnose (Colletotrichum lagenarium) and apple spot ( Alternaria leaf spot) six common agricultural plant pathogenic fungi were used as the experimental objects, and the antibacterial activity of all synthesized target compounds was tested by the method of mycelial growth rate, and the preliminary screening of antibacterial activity was carried out.
[0083] Experimental equipment: Petri dish (Hefei Xinenyuan Biotechnology Co., Ltd.), autoclave (TOMY SX-700), electrothermal constant temperature biochemical incubator (Shanghai Jinghong Experimental Equipment Co., Ltd.), eppendrof pipette gun, double-sided double-sided Purification workbench (Suzhou Purification Equipment Co., Ltd.), hole punch, etc.
[0084] Preparation of experiment...
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