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Sample pretreatment method, detection method and kit for folic acid and metabolite thereof

A detection method and metabolite technology, which are applied in the field of sample pretreatment methods, detection methods and kits of folic acid and its metabolites, can solve the problem of inability to intuitively understand the distribution and storage of folic acid, inability to understand the status of active folic acid, and inability to activate folic acid. Types and other problems, to achieve the effect of convenient and fast detection process, suitable for promotion, and enhanced accuracy and precision

Pending Publication Date: 2022-06-21
北京豪思生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the folic acid detection method used in clinical practice is mainly erythrocyte folic acid by chemiluminescence. Compared with other detection methods, the precision is poor. type; the detection of two MTHFR gene loci can intuitively judge the genotype at most, but infer the activity of the enzyme, and cannot intuitively understand the distribution and storage of folic acid caused by the enzyme activity, and thus cannot understand the clinical effects of folic acid supplementation. The situation of active folic acid that really works will bring troubles to the follow-up clinical guidance

Method used

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  • Sample pretreatment method, detection method and kit for folic acid and metabolite thereof
  • Sample pretreatment method, detection method and kit for folic acid and metabolite thereof
  • Sample pretreatment method, detection method and kit for folic acid and metabolite thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A method for detecting folic acid and its metabolites, comprising the following steps.

[0071] (1) Detection of hematocrit HCT in blood samples

[0072] In this experiment, the hematocrit (HCT) detection function of the hemoglobin meter is used to detect the whole blood sample. The detection of HCT includes but is not limited to this method. The advantage of this method is that it is fast, simple and easy to master, but compared with traditional methods The HCT measured by the centrifugation method has a certain deviation, which is within the acceptable range in this experimental application.

[0073] (2) Sample pretreatment:

[0074] Freeze-thaw lysis; subpackage the sample, which can effectively reduce the freeze-thaw test in the later retest process, which is beneficial to the storage stability of red blood cell folic acid. Put the aliquoted 100 μL whole blood sample into a -80°C freezer for rapid freezing for at least 30 minutes, and then return to room temperatu...

Embodiment 2

[0095] A detection method for folic acid and its metabolites is roughly the same as that in Example 1, except that the concentration of ascorbic acid in the diluent (same protective agent) is different, and the difference is as follows: the mass fraction of ascorbic acid is 0.1%.

Embodiment 3

[0097] A detection method for folic acid and its metabolites is roughly the same as that in Example 1, except that the concentration of ascorbic acid in the diluent (same protective agent) is different, and the difference is as follows: the mass fraction of ascorbic acid is 0.3%.

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Abstract

The invention discloses a sample pretreatment method for folic acid and metabolites thereof, a detection method and a kit, relates to the technical field of biological detection, and provides a detection method for detecting folic acid and metabolites thereof. The folic acid and the metabolites thereof in the sample have good stability, and the accuracy and the precision of liquid chromatography-tandem mass spectrometry detection of the folic acid and the metabolites thereof are effectively enhanced. According to the method, the content of folic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid and 5-formyltetrahydrofolic acid can be detected at the same time, then the total folic acid content and the active folic acid ratio can be obtained, the method has the advantages of being high in stability, good in precision and the like, the whole detection process is convenient and rapid, and the method is suitable for popularization.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular, to a sample pretreatment method, detection method and kit of folic acid and its metabolites. Background technique [0002] Folic acid is composed of pteridine, para-aminobenzoic acid and L-glutamic acid, also called pteroylglutamic acid. It is a water-soluble vitamin that is necessary for the growth and reproduction of human cells. It is a nutritional supplement for pregnant women. Folic acid supplementation can significantly reduce the incidence of neonatal neural tube defects and also help reduce the risk of cardiovascular and cerebrovascular diseases. [0003] Serum folic acid is considered to be an indicator of recent folic acid nutritional status, and serum folic acid levels alone cannot differentiate between transient dietary folic acid insufficiency and chronic folic acid deficiency; red blood cell folic acid levels can reflect chronic or long-term (within 4 mon...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02G01N30/72G01N33/82
CPCG01N30/06G01N30/02G01N30/72G01N33/82G01N2030/062G01N2030/067G01N2030/027
Inventor 李云龙栗琳邵立军刘胜敏孙敬茹周立丁亮钱俊伯
Owner 北京豪思生物科技股份有限公司
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