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Application of metformin in preparation of medicine for treating testicular function decline

A metformin and testicular technology, applied in the field of metformin use, can solve problems such as homeostasis imbalance, decline, and decreased body clearance ability.

Pending Publication Date: 2022-06-24
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the occurrence of aging, the body's clearance ability decreases, the homeostasis of the internal environment is out of balance, the activity of autophagy decreases with age, and the mitophagy mediated by the PINK1 / Parkin pathway may also decrease.
Because of the existence of the PINK1 / Parkin signaling pathway, metformin may prevent or improve aging by activating autophagy, but there is no relevant report yet

Method used

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  • Application of metformin in preparation of medicine for treating testicular function decline
  • Application of metformin in preparation of medicine for treating testicular function decline
  • Application of metformin in preparation of medicine for treating testicular function decline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Grouping, modeling and administration of rats

[0036] In this experiment, D-galactose was used to prepare the subacute aging rat model. The subacute aging rat model constructed by this method showed symptoms similar to those of natural aging. The principle is that continuous injection of D-galactose results in the accumulation of D-galactose in animals, the increase of intracellular osmotic pressure, resulting in cell swelling, causing cell metabolism disorders and dysfunction, and finally leading to the occurrence of aging.

[0037] Thirty 8-week-old SPF healthy male SD rats with a weight of 200±20g were selected. The rats were divided into blank control group, aging model group and Met drug treatment group by random number table method, 10 rats in each group.

[0038] The rats in the aging model group and the Met-treated group were given subcutaneous injection of D-galactose in the neck every day at a dose of 125 mg / kg, and the rats in the blank control g...

Embodiment 2

[0043] Example 2: The effect of Met on testicular weight and testicular index in subacute aging rats

[0044] The rats were killed after weighing the body weight of the rats, and the bilateral testicular tissues were quickly taken, the testis weight was weighed, and the testis index was calculated.

[0045] Testicular index = mean bilateral testicular weight / body weight.

[0046] The effects of Met on testicular weight and testicular index in subacute aging rats are as follows: figure 1 As shown, compared with the control group, the testicular weight and testis index of the rats in the model group were significantly decreased (P<0.01); After the Met intervention, the condition of the rat testis was improved.

Embodiment 3

[0047] Example 3: The effect of Met on serum hormones in subacute aging rats

[0048] The levels of testosterone (Testosterone, T), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in serum samples of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA). The ELISA kit was purchased from Shanghai Jianglai Biotechnology Co., Ltd., and the operation was carried out in strict accordance with the requirements of the kit instructions.

[0049] The result is as figure 2 As shown, compared with the control group, the serum T, FSH and E2 levels in the aging model group were significantly decreased (P<0.05); the LH level was significantly increased (P<0.01). After Met intervention, serum T, FSH and E2 levels all increased to varying degrees; LH level showed a downward trend (P<0.01), indicating that Met intervention improved the aging-induced testicular function decline and spermatogenesis in rats. The situation has improved.

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Abstract

The invention discloses a novel application of metformin, namely an application of metformin in preparation of a medicine for treating testicular function decline. It is disclosed that metformin can prevent or improve testicular hypofunction by activating autophagy and / or up-regulating expression of testosterone synthesis related enzymes. Tests prove that the metformin can be used for preparing a testis autophagy-related protein and testosterone synthesis-related protein by adjusting autophagy and / or up-regulating expression of testosterone synthesis-related enzyme from the aspects of testis weight of a rat, testis index change level, rat serum hormone level, rat testis form change and expression conditions of testis autophagy-related protein and testosterone synthesis-related protein. And testis hypofunction is prevented or improved.

Description

technical field [0001] The present application relates to the technical field of metformin use, in particular to the application of metformin in the preparation of a drug for the treatment of testicular function decline. Background technique [0002] Metformin (Met), a classic hypoglycemic prescription drug, is the first-line drug for the treatment of type 2 diabetes, especially for overweight patients; Metformin also plays an anti-tumor effect in a low-sugar environment. Autophagy is a process involving the breakdown of the cell's own structure through the lysosomal mechanism. After cells receive an autophagy-inducing signal, a flat "liposome"-like membrane structure called Phagophore is formed somewhere in the cytoplasm. With the continuous extension of Phagophore, the organelles and other components in the cytoplasm are all wrapped into a closed structure called "autophagosome". After the autophagosome is formed, it can be fused with lysosome, the contents in the autoph...

Claims

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Application Information

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IPC IPC(8): A61K31/155A61P15/08
CPCA61K31/155A61P15/08
Inventor 马会明陈冬梅王静何瑞孙苗田瑞莹马文欣刘畅
Owner NINGXIA MEDICAL UNIV
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