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Dual-protein delivery carrier programmed drug delivery system and preparation method thereof

A technology of delivery carrier and drug delivery system, which is applied in the field of dual-protein delivery carrier program drug delivery system and its preparation, and can solve problems such as inappropriateness

Pending Publication Date: 2022-06-24
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is a sustained-release administration, the administration of KGN in the prior art is basically no longer releasing KGN within 15-30 days, which is compared to the repair period of osteoarthritis cartilage defects of about 2 months , is obviously inappropriate

Method used

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  • Dual-protein delivery carrier programmed drug delivery system and preparation method thereof
  • Dual-protein delivery carrier programmed drug delivery system and preparation method thereof
  • Dual-protein delivery carrier programmed drug delivery system and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0041] As an embodiment of the present invention, the collagen used in the hydrogel is the recombinant collagen described in Chinese Patent Application Publication CN1371919A, which has good water solubility and a molecular weight of 97,000 Da. The silk protein is extracted from silkworm cocoons. , the activity unit of glutamine transaminase is 200U / g. The preparation method of the hydrogel of the present embodiment comprises the following steps:

[0042] 1) Preparation of soluble fibroin: the cocoons are cleaned after removing the chrysalis, put into a 5g / L sodium carbonate solution and boiled for 30min to degumming, the degummed fibroin is washed with deionized water, dried in an oven, and a certain amount of The fibroin was dissolved in a ternary solution of calcium chloride / water / ethanol (molar ratio 1:8:2) at 80°C for 1 h, and then the mixed solution was poured into a dialysis bag (molecular weight cut-off 8000-12000) at room temperature. Dialyzed with distilled water fo...

Embodiment 2

[0046] As an embodiment of the present invention, the collagen used in the hydrogel is the recombinant collagen described in Chinese Patent Application Publication CN1371919A, which has good water solubility, a molecular weight of 97,000 Da, and bovine serum albumin of reagent grade. (produced by American Amresco Company), the activity unit of glutamine transaminase is 200U / g. The preparation method of the hydrogel of the present embodiment comprises the following steps:

[0047] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: KGN was dissolved in ethanol solvent at a concentration of 0.4 mg / mL, then EDC / NHS (molar ratio: 1:1) was added, and stirred for 2 hours. A bovine serum albumin solution was prepared with a concentration of 2%, the pH was adjusted to 8 with 0.2M NaOH, and stirred at room temperature for 30 minutes. The KGN-ethanol solution was added dropwise to the bovine serum albumin solution and stirred continuously for 12h. The mixed solution was ...

Embodiment 3

[0050] As an embodiment of the present invention, the collagen used in the hydrogel is the recombinant collagen described in Chinese Patent Application Publication CN1371919A, which has good water solubility, a molecular weight of 97,000 Da, and human serum albumin of pharmaceutical grade. (produced by Boya Biopharmaceutical Group Co., Ltd.), the activity unit of glutamine transaminase is 200U / g. The preparation method of the hydrogel of the present embodiment comprises the following steps:

[0051] 1) Preparation of drug-loaded human serum albumin nanoparticles: KGN was dissolved in ethanol solvent at a concentration of 0.4 mg / mL, then EDC / NHS (molar ratio: 1:1) was added, and stirred for 2 hours. A human serum albumin solution was prepared with a concentration of 2%, the pH was adjusted to 8 with 0.2M NaOH, and the solution was stirred at room temperature for 30 minutes. The KGN-ethanol solution was added dropwise to the human serum albumin solution with constant stirring f...

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Abstract

The invention discloses a dual-protein delivery carrier programmed drug delivery system and a preparation method thereof. The dual-protein delivery carrier programmed drug delivery system comprises nanoparticles of a first protein, a first drug and hydrogel of a second protein, wherein the nanoparticles of the first protein are dispersed in the hydrogel of the second protein and are covalently linked with the second protein, and the first drug is loaded on the nanoparticles of the first protein. The dual-protein delivery carrier programmed drug delivery system provided by the invention is a multi-drug co-delivery system, and can realize co-system programmed release of hydrophilic drugs and hydrophobic drugs and ultra-long-acting release of the hydrophobic drugs; the invention is especially suitable for the situation that the first drug is a hydrophobic drug and is administered in a sustained-release manner, and the second drug is a hydrophilic drug and is administered in a quick-release manner.

Description

technical field [0001] The technology relates to the technical field of biomedical materials, in particular to a dual-protein delivery carrier program drug delivery system and a preparation method thereof. The dual-protein delivery carrier programmatic drug delivery system can realize programmatic drug delivery in different release forms of two drugs, and can realize ultra-long-acting release drug delivery of hydrophobic drugs. Background technique [0002] Kartogenin (KGN) is a heterocyclic compound screened by Novartis in 2012 that can promote the selective differentiation of pluripotent mesenchymal stem cells into chondrocytes. It has been developed into a mainstream drug for the repair of cartilage defects in osteoarthritis. one. [0003] KGN is a hydrophobic drug that is insoluble in water, and such drugs usually require the use of drug delivery vehicles for administration. In addition, the repair of osteoarthritis cartilage defects requires a long period of time (for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K31/196A61K47/64A61K45/06A61K31/573A61P19/02A61P19/08
CPCA61K31/196A61K47/6931A61K47/643A61K47/64A61K47/6903A61K47/6435A61K45/06A61P19/02A61P19/08A61K31/573A61K2300/00
Inventor 范代娣雷桓
Owner NORTHWEST UNIV(CN)
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