Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compositions, kits, methods and systems for nucleic acid sample amplification

A nucleic acid sample and composition technology, applied in the field of nucleic acid sample amplification composition, can solve the problems of difficulty in implementing multiple PCR capture technology, primer coverage, uniformity, etc.

Pending Publication Date: 2022-07-01
GUANGDONG FAPON BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the more target regions to be captured by multiplex PCR, the coverage and uniformity of primer amplification will be affected, and the more difficult it is to realize the multiplex PCR capture technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compositions, kits, methods and systems for nucleic acid sample amplification
  • Compositions, kits, methods and systems for nucleic acid sample amplification
  • Compositions, kits, methods and systems for nucleic acid sample amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Compositions for amplification of nucleic acid samples, including primer set 9. Among them, phenylketonuria (Phenylketonuria) is a disease caused by PAH gene defect. Primers are creatively designed for 212 pathogenic loci on PAH to avoid primer dimers and specific amplification, taking into account high homogeneity and High coverage, and optimized to obtain primer set 9 (nucleotide sequences shown in SEQ ID NO: 163-SEQ ID NO: 190).

Embodiment 2

[0066] A composition for nucleic acid sample amplification, including primer set 9 and primer set 2. Among them, 21-hydroxylase deficient congenital adrenal hyperplasia (Adrenal hyperplasia, congenital, due to 21-hydroxylasedeficiency) is a disease caused by CYP21A2 gene defect, and primers are creatively designed for 10 pathogenic loci on CYP21A2 , avoiding primer-dimer and specific amplification, taking into account high uniformity and high coverage, and optimized to obtain primer set 2 (nucleotide sequence shown in SEQ ID NO:47-SEQ ID NO:60); primers Group 9 is the same as Example 1.

Embodiment 3

[0068] A composition for nucleic acid sample amplification, comprising primer set 9 and primer set 5. Among them, Glycogen Storage Disease II (Glycogen Storage Disease II) is a disease caused by GAA gene defect. Primers are creatively designed for 75 pathogenic loci on GAA to avoid primer dimerization and specific amplification. Taking into account high homogeneity and high coverage, primer set 5 (nucleotide sequences shown in SEQ ID NO: 97-SEQ ID NO: 138) was obtained by optimization; primer set 9 was the same as Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a composition, a kit, a nucleic acid amplification method, a library building method and a system for nucleic acid sample amplification, which can be used for detection of single gene disease related variation sites, and high homogeneity and / or high coverage can be obtained while primer dimer and non-specific amplification are avoided.

Description

technical field [0001] The present invention relates to the field of molecular detection, in particular, to compositions, kits, methods and systems for nucleic acid sample amplification. Background technique [0002] Monogenic genetic disease (abbreviation: monogenic disease) is a disease caused by a single gene defect, in line with Mendelian inheritance, so it is also called Mendelian genetic disease. It is a type of disease that causes serious damage to human health (death, disability or teratogenicity) and lacks effective diagnosis and treatment methods, and is also one of the main causes of birth defects. So far, more than 10,000 kinds of monogenic diseases have been discovered. According to the statistics of the World Health Organization (WHO), the comprehensive incidence rate is as high as 1 / 100. Monogenic diseases often do not show structural deformities during fetal development, and are difficult to detect in routine obstetric examinations, and many recessive geneti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 李洪洲马晓冰章瑞程关媛妹张钰王晓丽
Owner GUANGDONG FAPON BIOTECH CO LTD
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com