Compositions, kits, methods and systems for nucleic acid sample amplification
A nucleic acid sample and composition technology, applied in the field of nucleic acid sample amplification composition, can solve the problems of difficulty in implementing multiple PCR capture technology, primer coverage, uniformity, etc.
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Embodiment 1
[0064] Compositions for amplification of nucleic acid samples, including primer set 9. Among them, phenylketonuria (Phenylketonuria) is a disease caused by PAH gene defect. Primers are creatively designed for 212 pathogenic loci on PAH to avoid primer dimers and specific amplification, taking into account high homogeneity and High coverage, and optimized to obtain primer set 9 (nucleotide sequences shown in SEQ ID NO: 163-SEQ ID NO: 190).
Embodiment 2
[0066] A composition for nucleic acid sample amplification, including primer set 9 and primer set 2. Among them, 21-hydroxylase deficient congenital adrenal hyperplasia (Adrenal hyperplasia, congenital, due to 21-hydroxylasedeficiency) is a disease caused by CYP21A2 gene defect, and primers are creatively designed for 10 pathogenic loci on CYP21A2 , avoiding primer-dimer and specific amplification, taking into account high uniformity and high coverage, and optimized to obtain primer set 2 (nucleotide sequence shown in SEQ ID NO:47-SEQ ID NO:60); primers Group 9 is the same as Example 1.
Embodiment 3
[0068] A composition for nucleic acid sample amplification, comprising primer set 9 and primer set 5. Among them, Glycogen Storage Disease II (Glycogen Storage Disease II) is a disease caused by GAA gene defect. Primers are creatively designed for 75 pathogenic loci on GAA to avoid primer dimerization and specific amplification. Taking into account high homogeneity and high coverage, primer set 5 (nucleotide sequences shown in SEQ ID NO: 97-SEQ ID NO: 138) was obtained by optimization; primer set 9 was the same as Example 1.
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