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Preparation method and application of fentanyl medicine hair standard substance

A standard substance, flufentanil technology, applied in the field of analytical chemistry, can solve the problem of not finding fentanyl drug standard substances, lack of detection standard substances, and lack of reliability, accuracy and traceability of test results. Safeguard and other issues, to achieve the effect of simple and fast sample pretreatment process, good accuracy and easy operation

Pending Publication Date: 2022-07-05
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, neither the international standard substance database (COMAR) nor the national standard substance resource sharing platform has found the registration of fentanyl drug standard substances, and the lack of standard substances for testing has led to the reliability, accuracy and traceability of test results. Technical issues not guaranteed

Method used

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  • Preparation method and application of fentanyl medicine hair standard substance
  • Preparation method and application of fentanyl medicine hair standard substance
  • Preparation method and application of fentanyl medicine hair standard substance

Examples

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preparation example Construction

[0077] In the present invention, a preparation method of a fentanyl-type drug standard substance in a hair sample, the steps are as follows:

[0078] (1) Collect adult hair samples with no history of illegal drug use, wash the collected hair samples with pure water and chromatographic grade methanol three times each, dry at room temperature and cut them into 2cm pieces.

[0079] (2) Preparation of soaking solution: weighing acetylfentanyl, isobutyrylfentanyl, p-fluorofentanyl, orfentanyl, thiofentanyl, 4-fluorobutyrylfentanyl, tetrahydrofuranfentanyl, fentanyl Nine kinds of fentanyl-type drugs, fentanyl and desmethylfentanyl, were placed in a container, distilled water and dimethyl sulfoxide solution were added, and the mixture was uniformly mixed by ultrasonic, which was a drug-dimethyl sulfoxide solution.

[0080] GC-MS / MS for acetylfentanyl, isobutyryl fentanyl, p-flufentanyl, orfentanyl, thiofentanyl, 4-fluorobutyryl fentanyl, tetrahydrofuran fentanyl, fentanyl and Demeth...

Embodiment 1

[0104] Qualitative analysis and determination of purity of pure fentanyl-like drugs.

[0105] Acetylfentanyl, isobutyrylfentanyl, p-fluorofentanyl, orfentanyl, thiofentanyl, 4-fluorobutyrylfentanyl, tetrahydrofuranfentanyl, fentanyl and desmethylfentanyl which The pure raw materials of 9 kinds of fentanyl drugs were provided by the Physical Evidence Identification Center of the Ministry of Public Security, and were sealed and stored in a 4°C refrigerator after vacuum packaging. According to the gravimetric method, a single standard solution with high concentration was prepared using methanol as a solvent, and stored in a refrigerator. During the experiment, according to the analysis requirements, use methanol to dilute to the desired concentration.

[0106] GC-MS / MS was used to qualitatively analyze 9 kinds of pure fentanyl drugs. Instrument: Agilent 7890A-7000 Triple Quadrupole GC-MS; Chromatographic column: DB-5MS column (30m×0.25mm×0.25μm); Injection port temperature: 280...

Embodiment 2

[0111] Preparation of hair standards.

[0112]Weigh approximately 30mg of Acetylfentanyl, Isobutyrylfentanyl, p-Fluorfentanyl, Orfentanil, Thiofentanyl, 4-Fluorobutyrylfentanyl, THF, Fentanyl and Demethylfentanyl Fentanyl 9 fentanyl-like drugs are placed in 1000mL bottles. Add 500 mL of distilled water and 500 mL of dimethyl sulfoxide solution (containing 0.02 mol / L hydrochloric acid), ultrasonicate for 5 minutes to make them evenly mixed, and after cooling, soak 40 g of hair in it, and place it in the dark at room temperature. During the preparation of the hair standard material, a part of the samples were taken on the 7th day, the 15th day, the 20th day, the 22nd day, the 24th day, and the 25th day after soaking, respectively, and analyzed by LC-MS / MS. The results showed that within 7-22 days, the content of the drug increased with the soaking time, and when the soaking time reached 24 days, the highest concentration of the drug entered the hair. After soaking for 24 days,...

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Abstract

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method and application of nine fentanyl drug standard substances in a hair sample, and the preparation method comprises the following steps: collecting an adult hair sample, washing, drying, soaking, cleaning, drying, crushing into dark brown powder and the like. The standard substance meets the requirements of forensic and clinical laboratories for fentanyl drug standard substances, the preparation method is simple, the valuing method is high in sensitivity, good in accuracy and high in specificity, and the standard substance can be used as a valuing method for the fentanyl drug matrix standard substance in hair and used for research and development of the standard substance. The method is applied to forensic abuse drug detection and laboratory quality control.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a preparation method and application of 9 kinds of fentanyl drug standard substances in a hair sample. Background technique [0002] As an anesthesia adjuvant, fentanyl has been widely used in surgical operations since the 1960s because of its fast onset, short duration and strong analgesic effect. As an opioid, fentanyl can activate the opioid receptors in the human body, which has excitatory and stimulating effects, and has strong addiction and tolerance. It is precisely because of its heroin-like effect that fentanyl as a drug is used for drug abuse in some countries, and the phenomenon of fentanyl abuse also appears. The abuse of fentanyl will not only cause harm to the human body, but even lead to death, the lethal dose for adults is only a few milligrams. [0003] In biological detection, hair, as an unconventional biological detection material, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N2030/045
Inventor 苏福海韦棋李红梅
Owner NAT INST OF METROLOGY CHINA
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