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Soybean regulatory elements and uses thereof

A technology of nucleotide sequences and expression cassettes, applied in the field of regulatory elements, can solve problems such as limited selection

Pending Publication Date: 2022-07-12
SYNGENTA PARTICIATIONS AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The choice of such promoters and terminators is still limited

Method used

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  • Soybean regulatory elements and uses thereof
  • Soybean regulatory elements and uses thereof
  • Soybean regulatory elements and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0109] Example 1: Identification and Characterization of Novel Promoter Sequences

[0110] method

[0111] Using reference genomes from wild Soybean species (Glycine max, Glycine max, Penghu Soybean and Glycine max) and based on the analysis of certain genes from soybean: ubiquitin (Ubi), s-adenosylmethionine Identification of orthologs of (SAM), actin depolymerization factor (ADF3), and translation elongation factor EF-1α (EF1a) resulted in candidate promoter sequences. Additional candidate promoter sequences were identified using RNAseq data to identify genes in soybean expressed between 12-18 log2 normalized data, and putative promoter sequences from these genes were added as candidates. Candidate promoter sequences are 2 kb regions upstream of the predicted or known translation start site. Candidate promoter sequences are expected to contain basal promoter elements, as well as the 5'UTR and possibly introns (depending on the predicted or known structure of the coding s...

example 2

[0137] Taken together, these data show that the four selected candidate promoters are highly expressed in a variety of tissues and are expected to be useful as constitutive promoters for use in expression constructs in soybean. Example 2: Identification and Characterization of Novel Terminator Sequences

[0138] method

[0139] Candidate terminator sequences were derived from the genes tested in Example 1 and tested for terminator activity. Candidate terminator sequences are 1 kb regions downstream of predicted or known translation termination sites. Candidate terminator sequences are expected to contain the 3' UTR and possibly additional 3' non-transcribed sequences (depending on the predicted or known structure of the coding sequence).

[0140] Vectors were designed for each candidate terminator, each containing Bx9 as a reporter gene and the promoter sequence prGmSAMS (SEQ ID NO: 7). Vectors were first screened by transient transformation using the method described in ...

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Abstract

Provided herein are regulatory elements, such as promoters and terminators, derived from or obtained from a variety of Soybean species. Such regulatory elements are useful in expression cassettes in plants such as soybean. Such expression cassettes generally contain promoter and terminator sequences to control the expression of each coding sequence. The use of promoters and terminators with medium to high constitutive expression may be required for certain traits, such as insect resistance and herbicide tolerance.

Description

[0001] Related applications [0002] This application claims priority to Provisional Application 62 / 939,762, filed November 25, 2019, which is incorporated herein by reference in its entirety. technical field [0003] The present invention relates to regulatory elements, such as promoters and terminators, which can be used in expression cassettes in plants such as soybean. [0004] sequence listing [0005] This application is accompanied by a sequence listing named 82000_ST25.txt created on November 16, 2020, which is approximately 54 kb in size. This Sequence Listing is incorporated herein by reference in its entirety. This Sequence Listing is filed with this application via EFS-Web and complies with 37 C.F.R. §1.824(a)(2)-(6) and (b). Background technique [0006] Transgenic plants are an important source of desirable traits such as insect resistance and herbicide tolerance. To generate such traits, one or more nucleic acids are typically introduced into plants contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82
CPCC12N15/8222C12N15/8271
Inventor 周爱灵J·科恩M·S·亚纳尔孙跃进陈钟颖阙求登Y·L·秦
Owner SYNGENTA PARTICIATIONS AG