Application of cowherb seed flavonoid glycoside in preparation of medicine for treating sepsis
A Wangbuliuxing flavonoid glycoside and sepsis technology, applied in the field of biomedicine, can solve the problems such as no reports on the prevention and treatment of sepsis by VA, and achieve the effects of improving pulmonary edema, improving survival rate, and improving pathology.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0055] Example 1: Studying the effect of VA concentration on cell viability in vitro
[0056] The in vitro cell model was constructed with RAW264.7 cells, and the RAW264.7 cells were cultured with medium containing different concentrations of VA, and the MTT colorimetric method was used to detect the cell survival and growth status to judge the cell viability. Among them, the concentration of VA is 0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 200, 400 μM, and the test results are as follows figure 1 shown. from figure 1 It can be seen that when the concentration of VA is 0-400 μM, there is no obvious toxicity of VA to RAW264.7 cells.
Embodiment 2
[0057] Example 2: Study the effect of VA on LPS (lipopolysaccharide)-induced in vitro cellular inflammatory response
[0058] RAW264.7 cells were cultured in the medium of different experimental groups, and the medium was grouped as follows: normal control group (NC), VA high-dose group (VA 100μM), LPS model group (LPS 1μg / ml), LPS model+ VA low-dose group (LPS 1 μg / ml+VA 25 μM), LPS model+VA medium-dose group (LPS 1 μg / ml+VA 50 μM), LPS model+VA high-dose group (LPS 1 μg / ml+VA 100 μM).
[0059] After culturing for 24 h, the supernatant of RAW264.7 cells was collected, the RNA in the supernatant was extracted, and the IL-1β, IL-6, TNF-α, etc. in the supernatant were determined by Real-time PCR. The expression level of inflammatory factor mRNA, the measurement results are as follows figure 2 shown ( figure 2 A, 2B, and 2C correspond to IL-1β, IL-6, and TNF-α, respectively). from figure 2 It can be seen that VA significantly reduced the mRNA expression levels of IL-1β, IL...
Embodiment 3
[0061] Example 3: Study the effect of VA on NF-κB signaling of LPS-induced cellular inflammatory response signaling pathway in vitro
[0062] Western blot method was used to detect RAW264 cultured in normal control group (NC), VA high-dose group (VA 100 μM), LPS model group (LPS 1 μg / ml), and LPS model + VA high-dose group (LPS 1 μg / ml + VA 100 μM). .7 The NF-κB inflammatory signaling pathways such as p-P65, P65, NLRP3, C-Caspase-1, and β-actin in cells were measured, and p-P65 / P65, NLRP3, and C-Caspase-1 were used as the abscissas. The relative protein expression level is the ordinate, and the expression levels of NF-κB inflammatory signaling pathway proteins in different experimental groups are obtained. The measurement results are as follows: Figure 4 shown, indicating that VA significantly inhibited LPS-induced expression of cellular NF-κB inflammatory signaling pathway proteins.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com