Application of cowherb seed flavonoid glycoside in preparation of medicine for treating sepsis

A Wangbuliuxing flavonoid glycoside and sepsis technology, applied in the field of biomedicine, can solve the problems such as no reports on the prevention and treatment of sepsis by VA, and achieve the effects of improving pulmonary edema, improving survival rate, and improving pathology.

Active Publication Date: 2022-07-15
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have proved that VA has the functions of activating blood circulation, dredging menstrual flow, reducing breast swelling, diuresis and treating stranguria, etc., but there are no reports on the prevention and treatment of sepsis with VA.

Method used

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  • Application of cowherb seed flavonoid glycoside in preparation of medicine for treating sepsis
  • Application of cowherb seed flavonoid glycoside in preparation of medicine for treating sepsis
  • Application of cowherb seed flavonoid glycoside in preparation of medicine for treating sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Studying the effect of VA concentration on cell viability in vitro

[0056] The in vitro cell model was constructed with RAW264.7 cells, and the RAW264.7 cells were cultured with medium containing different concentrations of VA, and the MTT colorimetric method was used to detect the cell survival and growth status to judge the cell viability. Among them, the concentration of VA is 0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 200, 400 μM, and the test results are as follows figure 1 shown. from figure 1 It can be seen that when the concentration of VA is 0-400 μM, there is no obvious toxicity of VA to RAW264.7 cells.

Embodiment 2

[0057] Example 2: Study the effect of VA on LPS (lipopolysaccharide)-induced in vitro cellular inflammatory response

[0058] RAW264.7 cells were cultured in the medium of different experimental groups, and the medium was grouped as follows: normal control group (NC), VA high-dose group (VA 100μM), LPS model group (LPS 1μg / ml), LPS model+ VA low-dose group (LPS 1 μg / ml+VA 25 μM), LPS model+VA medium-dose group (LPS 1 μg / ml+VA 50 μM), LPS model+VA high-dose group (LPS 1 μg / ml+VA 100 μM).

[0059] After culturing for 24 h, the supernatant of RAW264.7 cells was collected, the RNA in the supernatant was extracted, and the IL-1β, IL-6, TNF-α, etc. in the supernatant were determined by Real-time PCR. The expression level of inflammatory factor mRNA, the measurement results are as follows figure 2 shown ( figure 2 A, 2B, and 2C correspond to IL-1β, IL-6, and TNF-α, respectively). from figure 2 It can be seen that VA significantly reduced the mRNA expression levels of IL-1β, IL...

Embodiment 3

[0061] Example 3: Study the effect of VA on NF-κB signaling of LPS-induced cellular inflammatory response signaling pathway in vitro

[0062] Western blot method was used to detect RAW264 cultured in normal control group (NC), VA high-dose group (VA 100 μM), LPS model group (LPS 1 μg / ml), and LPS model + VA high-dose group (LPS 1 μg / ml + VA 100 μM). .7 The NF-κB inflammatory signaling pathways such as p-P65, P65, NLRP3, C-Caspase-1, and β-actin in cells were measured, and p-P65 / P65, NLRP3, and C-Caspase-1 were used as the abscissas. The relative protein expression level is the ordinate, and the expression levels of NF-κB inflammatory signaling pathway proteins in different experimental groups are obtained. The measurement results are as follows: Figure 4 shown, indicating that VA significantly inhibited LPS-induced expression of cellular NF-κB inflammatory signaling pathway proteins.

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Abstract

The invention relates to biological medicine, and discloses application of cowherb seed flavonoid glycoside in preparation of a medicine for treating sepsis, the cowherb seed flavonoid glycoside has the beneficial effects that the cowherb seed flavonoid glycoside is used for preparing the medicine for treating sepsis, the expression and secretion level of cell inflammatory factors can be reduced, and the effect of treating sepsis can be improved. An FKBP5/NF-kappa B inflammation signal channel of the cell is inhibited; the compound also can reduce the expression levels of inflammatory factors IL-1beta, IL-6 and TNF-alpha and chemotactic factors MCP-1 in serum of animals infected with sepsis, improve the pathology of kidney tissues and lung tissues of the animals infected with sepsis, inhibit the expression of NF-kappa B signaling pathways such as p-P65, NLRP3 and C-caspase-1 in the kidney tissues, reduce the levels of creatinine and urea nitrogen in serum of the kidney tissues, improve pulmonary edema and improve the curative effect of the kidney tissues. The survival rate of animals infected with sepsis is improved, and the kit has a potential treatment effect on sepsis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of a flavonoid glycoside of Wangbuliu in preparing a medicine for treating sepsis. Background technique [0002] Sepsis, a systemic inflammatory response syndrome caused by infection, is one of the leading causes of death worldwide. Critically ill patients account for a large proportion of sepsis patients. In high-income countries, 3-10 per 1,000 people develop sepsis each year. According to statistics, about 11% of ICU patients will develop sepsis, but there is no unified standard for the treatment strategy of sepsis. The current commonly used treatment strategies include fluid resuscitation, anti-infective therapy, application of glucocorticoids, and hemodialysis, but there are still controversies. Despite the continuous improvement of medical conditions, the mortality rate of sepsis still reaches 25-30%, and the mortality rate even reaches 40% when the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/04A61P29/00
CPCA61K31/7048A61P31/04A61P29/00Y02A50/30
Inventor 温家根马文贤王月悦黄申卓凡赵贵涛孟晓明吴婷妮
Owner ANHUI MEDICAL UNIV
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