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Preparation method of conductive tissue engineering material carrying traditional Chinese medicine monomer and neural stem cells for treating spinal cord injury

A technology of neural stem cells and spinal cord injury, applied in the direction of prosthesis, medical science, etc., can solve the problem that the physiological and mechanical properties of the spinal cord cannot be well matched, the pathological response of secondary spinal cord injury cannot be suppressed, and the promotion of nerve repair is limited, etc. It can improve the spinal cord microenvironment, the preparation method is simple and feasible, and the effect is reliable.

Pending Publication Date: 2022-08-05
QINGDAO MUNICIPAL HOSPITAL
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The improvement of the spinal cord microenvironment is not significant;
[0005] (2) The electroactive hydrogel alone has limited effect on nerve repair after spinal cord injury;
[0006] (3) It does not have neuroprotective effect and cannot inhibit the pathological response of secondary spinal cord injury;
[0007] (4) It cannot match the physiological and mechanical properties of the spinal cord well

Method used

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  • Preparation method of conductive tissue engineering material carrying traditional Chinese medicine monomer and neural stem cells for treating spinal cord injury

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Embodiment 1

[0053] like figure 1 As shown, a preparation method of a conductive tissue engineering material carrying traditional Chinese medicine monomers and neural stem cells for treating spinal cord injury is characterized in that, comprising the following specific steps:

[0054] S1, preparation of electroactive hydrogel;

[0055] S2. Preparation of high-dose ligustrazine-electroactive hydrogel;

[0056] S3. Preparation of electroactive hydrogel with ligustrazine and neural stem cells.

[0057] The specific content of step S1 includes:

[0058] (1) Blending

[0059] ①Add 1ml deionized water to the 4mlEP tube, and wrap the bottle body and lid with tin foil;

[0060] ② Take out the photoinitiator from the refrigerator at 4 degrees Celsius, untie the sealing film, weigh in the dark room, 0.01g of the photoinitiator, and then pour it into a centrifuge tube (protect from light);

[0061] ③ Shake it well and place it in a 60°C water bath to heat it, and dissolve it into a clear liquid ...

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Abstract

The invention provides a preparation method of a conductive tissue engineering material carrying traditional Chinese medicine monomers and neural stem cells for treating spinal cord injury, which is characterized by comprising the following specific steps: S1, preparing electroactive hydrogel; s2, preparing a high dose of ligustrazine-electroactive hydrogel; s3, preparing electroactive hydrogel carrying ligustrazine and neural stem cells; the electroactive hydrogel is co-cultured with embryonic neural stem cells by using a cell transplantation technology, so that the embryonic neural stem cells are loaded on the electroactive hydrogel, and a novel composite material with excellent physicochemical properties such as conductivity, histocompatibility, degradability and spinal cord matching property is constructed; the purity, the cell activity and the cell proliferation speed of the embryonic neural stem cells can be remarkably improved; the differentiation of the embryonic neural stem cells can be inhibited; and the pluripotent differentiation potential of the embryonic neural stem cells can be maintained.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a preparation method of a conductive tissue engineering material carrying traditional Chinese medicine monomers and neural stem cells for treating spinal cord injury. Background technique [0002] The difficulty of nerve repair after spinal cord injury has always been a difficult problem to solve. Conductive tissue engineering materials show good application prospects in the field of nerve repair and regeneration. However, the role of electrical tissue engineering materials is limited to improving the local electrical microenvironment of the spinal cord, which is beneficial to the repair of electrical conduction pathways. [0003] Currently, the most widely used conductive tissue engineering materials are electroactive hydrogels, which can fill the spinal cord cavity, provide an electrical microenvironment, and serve as a good drug-carrying platform. However, it al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/50A61L27/54A61L27/58
CPCA61L27/3834A61L27/3878A61L27/50A61L27/54A61L27/58A61L2430/32A61L2300/204A61L2300/602
Inventor 劳克诚范筱胡光亮
Owner QINGDAO MUNICIPAL HOSPITAL
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