Method for culturing natural multi-colour lawn
A cultivation method and turfgrass technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as single color
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Embodiment 1
[0023] Cultivation of pure natural colored tall fescue by chemical mutagenesis of dry seeds:
[0024] In September, 1998, 0.5 kilograms of tall fescue dry seeds with a moisture content of 13.5% were taken as starting materials, and after soaking the seeds in distilled water for 16 hours, they were treated with chemical mutagen sodium azide, and the dose of treatment was 2.0mMh ( The concentration of sodium azide was 1.0 mM, the treatment time was 2 hours, and the treatment temperature was 25° C.). Live Mutagenesis Contemporary M 0 Seeds, at the end of May of the following year, all mutagenic generation M 1 The seeds of the plant. In mid-September 1999, the second-generation M 2 When the seedlings grow to the two-leaf stage, screening can clearly identify leaf color variants that are significantly different from normal green. In May 2000, the mature seeds of the leaf color variant were harvested by the single plant method. In June 2000, the temperature and light sensitivit...
Embodiment 2
[0026] Cultivation of pure natural colored bentgrass by radiation mutagenesis of young stems:
[0027] In July, 1998, the top young stems of bentgrass (about 2.0-3.5 cm in length) were cut from the field, sterilized on the surface, cut into small sections (about 0.5 cm in length), inoculated in 1 / 2MS solid medium, and waited for After inducing a cure, give 60 Co-γ-ray radiation, the treatment dose is 5Gy. After one week of radiation treatment, subculture once, and then transfer to hormone-free 1 / 2 MS medium for differentiation. After the differentiated seedlings grow fully, they are transferred to the greenhouse to continue to grow, and then transplanted to the field by division, planted in isolation, and harvested separately. In vitro mutagenesis generation (M 1 R 1 ) to screen out leaf color variants. In September 1999, the second generation (M 2 R 2 ) group consisting of single plants, screen the leaf color variants, and harvest seeds according to the single plant me...
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